The Protective Effects of Pueraria Radix against Chronic Alcohol-induced Muscle Atrophy in Rats (알콜로 유도된 흰쥐의 근위축에서 葛根의 보호 작용과 그 기전에 대한 고찰)

김범회

The Protective Effects of Pueraria Radix against Chronic Alcohol-induced Muscle Atrophy in Rats

Journal of Korean Medicine Rehabilitation
Abbr : JKMR
2017, 27(2), pp.1-8
Publisher : The Korean Academy Of Oriental Rehabilitation Medicine
Research Area : Medicine and Pharmacy > Korean Medicine

김범회 ¹

¹동의대학교

Accredited

ABSTRACT

ObjectivesEthanol is a potent inhibitor of muscle protein synthesis. Muscle mass is regulated by the balance between rates of protein synthesis and protein breakdown. Both acute and chronic alcohol consumption inhibits synthesis to a greater extent than degradation. Protein synthesis is more intensely decreased in type II fibers than in type I fibers. Apoptosis has been shown to occur frequently in a variety of tissues in response to chronic alcohol feeding. Increased muscle fiber apoptosis has been shown in alcoholics with myopathy. Pueraria radix has been used for many disorders such as fevers, gastro-intestinal disorders, muscle aches, allergies, respiratory problems, skin problems, high blood pressure, migraine headaches, lowering cholesterol and treating chronic alcoholism. We therefore tested the hypothesis that oral treatment with Pueraria radix could reduce the ethanol-induced muscle atrophy. MethodsYoung male Sprague-Dawley rats were orally given 25% ethanol (5 ml/kg, body weight) daily with Ethanol for 4 weeks. Normal group was similarly administrated with saline. The Rats of Pueraria radix treated group (EtOH+PR) were orally administrated Pueraria radix water extract, and rats of EtOH group were given with the vehicle only. After 4 week, the morphology of gastrocnemius and plantaris muscles were assessed by hema-toxylin and eosin staining. The immunoreactivities of pre-apoptotic BAX and anti-apoptotic Bcl-2 proteins were also measured.ResultsThe muscles from rats of EtOH group represented a significant reduction in aver-age cross section area compared to Normal group. EtOH+PR group had increased fiber compared to the EtOH group. Moreover, to investigate the ethanol-induced muscular apoptosis, the immunohistochemical analysis of Bax and Bcl-2 was carried out. The treat-ment with Pueraria radix (EtOH+PR) significantly decreased BAX expression and increased Bcl-2 expression 4 weeks after ethanol administration when compared with Normal group. ConclusionsThese results suggest that Pueraria radix water extract has protective ef-fects on chronic alcohol induced myopathy. (J Korean Med Rehabil 2017;27(2):1-8)

KEYWORDS

Pueraria radix, Alcoholic myopathy, Gastrocnemius, Plantaris, BAX, Bcl-2

Citation status

* References for papers published after 2023 are currently being built.

[journal] Romero JC / 1994 / Chronic alcoholic myopathy and nutritional status / Alcohol 11(6) : 549~555

[journal] Preedy VR / 2001 / Alcoholic skeletal muscle myopathy : definitions, features, contribution of neuropathy, impact and diagnosis / Eur J Neurol 8(6) : 677~687

[journal] Preedy VR / 2001 / Alcoholic myopathy : biochemical mechanisms / Drug Alcohol Depend 63(3) : 199~205

[journal] Martin F / 1985 / Alcoholic skeletal myopathy, a clinical and pathological study / Q J Med 55(218) : 233~251

[journal] Hoek JB / 2002 / Ethanol, oxidative stress, and cytokine-induced liver cell injury / Alcohol 27(1) : 63~68

[journal] Urbano-Marquez A / 2004 / Effects of alcohol on skeletal and cardiac muscle / Muscle Nerve 30(6) : 689~707

[journal] Hajnoczky G / 2006 / Mitochondrial calcium signalling and cell death: approaches for assessing the role of mitochondrial Ca2+ uptake in apoptosis / Cell Calcium 40(5-6) : 553~560

[journal] Kroemer G / 1997 / The proto-oncogene Bcl-2 and its role in regulating apoptosis / Nat Med 3(6) : 614~620

[book] Lee SI / 1997 / Herbology / Eui Hak Sa : 65~66

[book] Kim HC / 2001 / Herbal pharmacology / Jip Moon Dang : 92~94

[journal] Wang S / 2016 / Puerarin protects against CCl4-induced liver fibrosis in mice : possible role of PARP-1inhibition / Int Immunopharmacol 38 : 238~245

[journal] Li R / 2013 / Puerarin, isolated from Kudzu root (Willd.), attenuates hepatocellular cytotoxicity and regulates the GSK-3β/NF-κB pathway for exerting the hepatoprotection against chronic alcohol-induced liver injury in rats / Int Immunopharmacol 17(1) : 71~78

[journal] Chen X / 2013 / Puerarin improves metabolic function leading to hepatoprotective effects in chronic alcohol-induced liver injury in rats / Phytomedicine 20(10) : 849~852

[journal] 김홍배 / 2009 / The Effects of Puerariae Radix on Allergic Inflammation / 대한한방소아과학회지 / 대한한방소아과학회 23(3) : 217~231

[journal] 장승욱 / 2009 / Effect of Puerariae Radix on Hind Limb Muscle Atrophy of Sciatic Nerve Transectioned Rats / 동의생리병리학회지 / 한의병리학회 23(2) : 405~411

[journal] 홍재의 / 2010 / Effects of Puerariae Radix extract on Cisplatin-Induced Apoptosis of Rat Mesangial Cells / 동의생리병리학회지 / 한의병리학회 24(2) : 220~227

[journal] Gao Y / 2016 / An isoflavonoid-enriched extract from Pueraria lobata(kudzu)root protects human umbilical vein endothelial cells against oxidative stress induced apoptosis / J Ethnopharmacol 193 : 524~530

[journal] Jung HW / 2017 / The Root Extract of Pueraria lobata and Its Main Compound, Puerarin, Prevent Obesity by Increasing the Energy Metabolism in Skeletal Muscle / Nutrients 9(1) : 1~13

[journal] Fujii H / 1985 / Liver microsomal drug metabolism in ethanol-treated hamsters / Biochem Pharmacol 34(21) : 3881~3884

[journal] Otis JS / 2007 / Oxidant-induced atrogin-1and transforming growth factor-beta1 precede alcohol-related myopathy in rats / Muscle Nerve 36(6) : 842~848

[book] Lee SI / 1990 / Herbal Formula Science / Young Lim Sa : 97~

[journal] Lieber CS / 1989 / Experimental methods of ethanol administration / Hepatology 10(4) : 501~510

[journal] Mezey E / 1980 / Alcoholic liver disease : roles of alcohol and malnutrition / Am J Clin Nutr 33(12) : 2709~2718

[journal] Woods SE / 1993 / Alcoholic hepatitis / Am Fam Physician 47(5) : 1171~1178

[journal] Herlong HF / 1994 / Approach to the patient with abnormal liver enzymes / Hosp Pract(Off Ed) 29(11) : 32~38

[journal] Otis JS / 2009 / Procysteine stimulates expression of key anabolic factors and reduces plantaris atrophy in alcohol-fed rats / Alcohol Clin Exp Res 33(8) : 1450~1459

[journal] Korzick DH / 2013 / Aging accentuates alcohol-induced decrease in protein synthesis in gastrocnemius / Am J Physiol Regul Integr Comp Physiol 304(10) : 887~898

[journal] Narula J / 1996 / Apoptosis in myocytes in end-stage heart failure / N Engl J Med 335(16) : 1182~1189

[book] Shin MK / 2008 / Oriental Medical Physiology / Jip Moon dang : 217~

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Journal of Korean Medicine Rehabilitation 2023 KCI Impact Factor : 0.6