@article{ART002339821},
author={Soo-Hwan Lee and Kim， Soon-joong},
title={Anti-inflammatory Effects of Gagamtongsoon-San Extract on Lipopolysaccharide(LPS)-Induced Inflammation in RAW264.7 Cells},
journal={Journal of Korean Medicine Rehabilitation},
issn={1229-1854},
year={2018},
volume={28},
number={2},
pages={37-45},
doi={10.18325/jkmr.2018.28.2.37}
TY - JOUR
AU - Soo-Hwan Lee
AU - Kim， Soon-joong
TI - Anti-inflammatory Effects of Gagamtongsoon-San Extract on Lipopolysaccharide(LPS)-Induced Inflammation in RAW264.7 Cells
JO - Journal of Korean Medicine Rehabilitation
PY - 2018
VL - 28
IS - 2
PB - The Korean Academy Of Oriental Rehabilitation Medicine
SP - 37
EP - 45
SN - 1229-1854
AB - Objectives This study was designed to investigate whether the Gagamtongsoon-San (GT) has an inhibitory effect and its mechanisms are associated with the iNOS and COX-2.
Methods Cytotoxic activity of GT extract on RAW264.7 cells was evaluated by using 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) solution. Inflammatory condition was induced by LPS. NO production was measured using Griess reagent system. The expressions of iNOS and COX-2 mRNA and protein were determined by realtime PCR. The concentrations of PGE2 were measured by an enzyme immunoassay (EIA).
Results The GT does not impair the cell viability in tested concentration 500 µg/ml or below. GT significantly reduced the NO production in a dose-dependent manner. GT 500 µg/ml also suppressed LPS-induced mRNA expressions of iNOS and COX-2. GT 500 µg/ml reduced the PGE2 secretion in LPS induced RAW264.7 cells.
Conclusions These outcomes show that GT extract has an anti-inflammatory activities. And also this conclusion can be the data that supports the GT's anti-inflammatory effect objectively. (J Korean Med Rehabil 2018;28(2):37-45)
KW - Gagamtongsoon-San(GT);Anti-inflammatory;RAW264.7;LPS
DO - 10.18325/jkmr.2018.28.2.37
ER -
Soo-Hwan Lee and Kim， Soon-joong. (2018). Anti-inflammatory Effects of Gagamtongsoon-San Extract on Lipopolysaccharide(LPS)-Induced Inflammation in RAW264.7 Cells. Journal of Korean Medicine Rehabilitation, 28(2), 37-45.
Soo-Hwan Lee and Kim， Soon-joong. 2018, "Anti-inflammatory Effects of Gagamtongsoon-San Extract on Lipopolysaccharide(LPS)-Induced Inflammation in RAW264.7 Cells", Journal of Korean Medicine Rehabilitation, vol.28, no.2 pp.37-45. Available from: doi:10.18325/jkmr.2018.28.2.37
Soo-Hwan Lee, Kim， Soon-joong "Anti-inflammatory Effects of Gagamtongsoon-San Extract on Lipopolysaccharide(LPS)-Induced Inflammation in RAW264.7 Cells" Journal of Korean Medicine Rehabilitation 28.2 pp.37-45 (2018) : 37.
Soo-Hwan Lee, Kim， Soon-joong. Anti-inflammatory Effects of Gagamtongsoon-San Extract on Lipopolysaccharide(LPS)-Induced Inflammation in RAW264.7 Cells. 2018; 28(2), 37-45. Available from: doi:10.18325/jkmr.2018.28.2.37
Soo-Hwan Lee and Kim， Soon-joong. "Anti-inflammatory Effects of Gagamtongsoon-San Extract on Lipopolysaccharide(LPS)-Induced Inflammation in RAW264.7 Cells" Journal of Korean Medicine Rehabilitation 28, no.2 (2018) : 37-45.doi: 10.18325/jkmr.2018.28.2.37
Soo-Hwan Lee; Kim， Soon-joong. Anti-inflammatory Effects of Gagamtongsoon-San Extract on Lipopolysaccharide(LPS)-Induced Inflammation in RAW264.7 Cells. Journal of Korean Medicine Rehabilitation, 28(2), 37-45. doi: 10.18325/jkmr.2018.28.2.37
Soo-Hwan Lee; Kim， Soon-joong. Anti-inflammatory Effects of Gagamtongsoon-San Extract on Lipopolysaccharide(LPS)-Induced Inflammation in RAW264.7 Cells. Journal of Korean Medicine Rehabilitation. 2018; 28(2) 37-45. doi: 10.18325/jkmr.2018.28.2.37
Soo-Hwan Lee, Kim， Soon-joong. Anti-inflammatory Effects of Gagamtongsoon-San Extract on Lipopolysaccharide(LPS)-Induced Inflammation in RAW264.7 Cells. 2018; 28(2), 37-45. Available from: doi:10.18325/jkmr.2018.28.2.37
Soo-Hwan Lee and Kim， Soon-joong. "Anti-inflammatory Effects of Gagamtongsoon-San Extract on Lipopolysaccharide(LPS)-Induced Inflammation in RAW264.7 Cells" Journal of Korean Medicine Rehabilitation 28, no.2 (2018) : 37-45.doi: 10.18325/jkmr.2018.28.2.37
