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Anti-inflammatory Effects of Gagamtongsoon-San Extract on Lipopolysaccharide(LPS)-Induced Inflammation in RAW264.7 Cells

  • Journal of Korean Medicine Rehabilitation
  • Abbr : JKMR
  • 2018, 28(2), pp.37-45
  • DOI : 10.18325/jkmr.2018.28.2.37
  • Publisher : The Korean Academy Of Oriental Rehabilitation Medicine
  • Research Area : Medicine and Pharmacy > Korean Medicine
  • Received : March 19, 2018
  • Accepted : April 2, 2018
  • Published : April 30, 2018

Soo-Hwan Lee 1 Kim, Soon-joong 2

1세명대학교 부속한방병원 한방재활의학과
2세명대학교

Accredited

ABSTRACT

Objectives This study was designed to investigate whether the Gagamtongsoon-San (GT) has an inhibitory effect and its mechanisms are associated with the iNOS and COX-2. Methods Cytotoxic activity of GT extract on RAW264.7 cells was evaluated by using 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) solution. Inflammatory condition was induced by LPS. NO production was measured using Griess reagent system. The expressions of iNOS and COX-2 mRNA and protein were determined by realtime PCR. The concentrations of PGE2 were measured by an enzyme immunoassay (EIA). Results The GT does not impair the cell viability in tested concentration 500 µg/ml or below. GT significantly reduced the NO production in a dose-dependent manner. GT 500 µg/ml also suppressed LPS-induced mRNA expressions of iNOS and COX-2. GT 500 µg/ml reduced the PGE2 secretion in LPS induced RAW264.7 cells. Conclusions These outcomes show that GT extract has an anti-inflammatory activities. And also this conclusion can be the data that supports the GT's anti-inflammatory effect objectively. (J Korean Med Rehabil 2018;28(2):37-45)

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