Effects of Essential Oils Extracted from Cnidii Rhizoma on Differentiation and Adipogenesis in 3T3-L1 Adiopocytes (천궁(川芎)의 정유 추출물이 3T3-L1 세포의 분화 및 지방 생성에 미치는 영향)

Soo-Min Choi (최수민); So Young Kim (김소영); Na-Ri Park (박나리); Jung-Min Kim (김정민); Doo-Hwa Yang (양두화); Chang-Hoon Woo (우창훈); Mi-Ryeo, Kim (김미려); Hee Duk An (안희덕)

doi:10.18325/jkmr.2018.28.3.13

Effects of Essential Oils Extracted from Cnidii Rhizoma on Differentiation and Adipogenesis in 3T3-L1 Adiopocytes

Journal of Korean Medicine Rehabilitation
Abbr : JKMR
2018, 28(3), pp.13-25
DOI : 10.18325/jkmr.2018.28.3.13
Publisher : The Korean Academy Of Oriental Rehabilitation Medicine
Research Area : Medicine and Pharmacy > Korean Medicine
Received : June 13, 2018
Accepted : July 5, 2018
Published : July 31, 2018

Soo-Min Choi ¹, So Young Kim ², Na-Ri Park ¹, Jung-Min Kim ¹, Doo-Hwa Yang ³, Chang-Hoon Woo ¹, Mi-Ryeo, Kim ², Hee Duk An ¹

¹대구한의대학교 한의과대학 한방재활의학과교실
²대구한의대학교 한의과대학 본초약리학교실
³대구한의대학교 한의과대학 한방재활의학과 교실

Accredited

ABSTRACT

Objectives We investigated anti-obesity effects of essential oils extracted from Cnidii Rhizoma (CR) in immature adipocytes to magnify it’s clinical therapeutic usage. Methods Essential oil of CR was extracted with ethyl acetate or petroleum ether and through steam distillation, respectively. Oil red-O staining for monitoring its inhibition effect on adipogenesis and differentiation in murine 3T3-L1 adipocytes and 3-(4,5-methylthiazol–2-yl)-2,5-diphenyletetra zolium bromide (MTT) assay for cell safety were done. Also phospho-adenosine monophosphate (AMP)-activted protein kinase (P-AMPK), AMP-activated protein kinase, phospho-acetyl-CoA carboxylase (P-ACC), acetyl-CoA carboxylase, peroxisome proliferator-activated receptor-α (PPAR-α), peroxisome proliferator-activated receptor-γ (PPAR-γ) and CCAAT/enhancer binding protein α (C/EBP-α) expressions as obesity-related factors were measured by western blot analysis. Results Protein expressions of P-AMPK, P-ACC and PPAR-α were increased in essential oils-treated adipocytes compared to those of control group, respectively. Furthermore, protein expressions of PPAR-γ and C/EBP-α were decreased in essential oils-treated adipocytes compared to those of control group, respectively. Conclusions These results demonstrate that essential oils of CR inhibit adipogenesis and differentiation. Also they promote the oxidation of fatty acids in adipocytes. Thus, results suggest that essential oils of CR could be used as a valuable material for anti-obesity therapeutics via control of lipid metabolism. (J Korean Med Rehabil 2018;28(3):13-25)

KEYWORDS

Cnidii Rhizoma, Essential oils, Adipogenesis, 3T3-L1 cells, Obesity

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This paper was written with support from the National Research Foundation of Korea.

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Journal of Korean Medicine Rehabilitation 2023 KCI Impact Factor : 0.6

Effects of Essential Oils Extracted from Cnidii Rhizoma on Differentiation and Adipogenesis in 3T3-L1 Adiopocytes

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* References for papers published after 2023 are currently being built.

Journal of Korean Medicine Rehabilitation 2023 KCI Impact Factor : 0.6

Effects of Essential Oils Extracted from Cnidii Rhizoma on Differentiation and Adipogenesis in 3T3-L1 Adiopocytes

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