@article{ART002643061},
author={Woo-Suk Shin and Kira Parichuk and Cha Yun Yeop},
title={Effect of Pahyeolsandong-tang (Poxiesanteng-tang) in Tibia Fracture-induced Mice},
journal={Journal of Korean Medicine Rehabilitation},
issn={1229-1854},
year={2020},
volume={30},
number={4},
pages={1-16},
doi={10.18325/jkmr.2020.30.4.1}
TY - JOUR
AU - Woo-Suk Shin
AU - Kira Parichuk
AU - Cha Yun Yeop
TI - Effect of Pahyeolsandong-tang (Poxiesanteng-tang) in Tibia Fracture-induced Mice
JO - Journal of Korean Medicine Rehabilitation
PY - 2020
VL - 30
IS - 4
PB - The Korean Academy Of Oriental Rehabilitation Medicine
SP - 1
EP - 16
SN - 1229-1854
AB - Objectives The main purpose of this study was to evaluate the bone healing effect of Pahyeolsandong-tang (PHT)(Poxiesanteng-tang) extract in tibia fracture-induced mice.
Methods PHT was extracted using a solution of 35% ethanol in 60°C for 8 hours. Mice were randomly divided into 4 groups (normal, control, PHT 50 and PHT 100). Mice of experimental groups were medicated with PHT 50 or 100 mg/kg for 7 to 21 days. To clarify the effect of bone fracture healing, relative messenger RNA (mRNA) expressions of osteocalcin (OCN), runt-related transcription factor 2 (Runx2), osterix (OSX), Sox9, collagen type Ⅱ alpha 1 chain (Col2a1), receptor activator of nuclear factor kappa-B ligand (RANKL), osteoprotegerin (OPG) were examined.
Results In in vitro experiment, relative mRNA expression of OCN, Runx2, Col2a1 was significantly increased in PHT treated group to compare with control differentiation group. In in vivo experiment, relative mRNA expression of OCN, Runx2, OSX, Sox9, Col2a1, RANKL, OPG was significantly increased in PHT treated group.
Conclusions This study showed that PHT accelerates bone fracture healing through the activation of osteoclasts and osteoblasts. It was showed that PHT significantly promotes osteoblasts differentiation by osteoblast differentiation markers such as OCN, Runx2, Col1a2. Also it was investigated that PHT had stimulatory effect on osteoblasts function through enhancing OCN, Runx2, OSX, Sox9, Col2a1 and, osteoclasts function through enhancing RANKL and OPG markers. PHT effectively promotes bone fracture healing process through activation of osteoblasts and osteoclasts. (J Korean Med Rehabil 2020;30(4):1-16)
KW - Pahyeolsandong-tang (Poxiesanteng-tang);Osteoblasts;Osteoclasts;Chondrogenesis;Fracture healing
DO - 10.18325/jkmr.2020.30.4.1
ER -
Woo-Suk Shin, Kira Parichuk and Cha Yun Yeop. (2020). Effect of Pahyeolsandong-tang (Poxiesanteng-tang) in Tibia Fracture-induced Mice. Journal of Korean Medicine Rehabilitation, 30(4), 1-16.
Woo-Suk Shin, Kira Parichuk and Cha Yun Yeop. 2020, "Effect of Pahyeolsandong-tang (Poxiesanteng-tang) in Tibia Fracture-induced Mice", Journal of Korean Medicine Rehabilitation, vol.30, no.4 pp.1-16. Available from: doi:10.18325/jkmr.2020.30.4.1
Woo-Suk Shin, Kira Parichuk, Cha Yun Yeop "Effect of Pahyeolsandong-tang (Poxiesanteng-tang) in Tibia Fracture-induced Mice" Journal of Korean Medicine Rehabilitation 30.4 pp.1-16 (2020) : 1.
Woo-Suk Shin, Kira Parichuk, Cha Yun Yeop. Effect of Pahyeolsandong-tang (Poxiesanteng-tang) in Tibia Fracture-induced Mice. 2020; 30(4), 1-16. Available from: doi:10.18325/jkmr.2020.30.4.1
Woo-Suk Shin, Kira Parichuk and Cha Yun Yeop. "Effect of Pahyeolsandong-tang (Poxiesanteng-tang) in Tibia Fracture-induced Mice" Journal of Korean Medicine Rehabilitation 30, no.4 (2020) : 1-16.doi: 10.18325/jkmr.2020.30.4.1
Woo-Suk Shin; Kira Parichuk; Cha Yun Yeop. Effect of Pahyeolsandong-tang (Poxiesanteng-tang) in Tibia Fracture-induced Mice. Journal of Korean Medicine Rehabilitation, 30(4), 1-16. doi: 10.18325/jkmr.2020.30.4.1
Woo-Suk Shin; Kira Parichuk; Cha Yun Yeop. Effect of Pahyeolsandong-tang (Poxiesanteng-tang) in Tibia Fracture-induced Mice. Journal of Korean Medicine Rehabilitation. 2020; 30(4) 1-16. doi: 10.18325/jkmr.2020.30.4.1
Woo-Suk Shin, Kira Parichuk, Cha Yun Yeop. Effect of Pahyeolsandong-tang (Poxiesanteng-tang) in Tibia Fracture-induced Mice. 2020; 30(4), 1-16. Available from: doi:10.18325/jkmr.2020.30.4.1
Woo-Suk Shin, Kira Parichuk and Cha Yun Yeop. "Effect of Pahyeolsandong-tang (Poxiesanteng-tang) in Tibia Fracture-induced Mice" Journal of Korean Medicine Rehabilitation 30, no.4 (2020) : 1-16.doi: 10.18325/jkmr.2020.30.4.1