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Healing Effect of Yukmijihwang-tang on Fracture Factor and Morphological Changes in Femur Fractured Mice

  • Journal of Korean Medicine Rehabilitation
  • Abbr : JKMR
  • 2020, 30(4), pp.17-30
  • DOI : 10.18325/jkmr.2020.30.4.17
  • Publisher : The Korean Academy Of Oriental Rehabilitation Medicine
  • Research Area : Medicine and Pharmacy > Korean Medicine
  • Received : October 7, 2020
  • Accepted : October 21, 2020
  • Published : October 31, 2020

Hyun-Seok Kim 1 Dong Hwi Jeon 1 Min Seok Oh 2

1대전대학교 한의과대학 한방재활의학과교실
2대전대학교 한의과대학 한방재활의학과 교실

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ABSTRACT

Objectives The purpose of this study is to evaluate the healing effect of Yukmijihwang- tang (YM) on femur fractured mice. Methods Mice were randomly divided into 6 groups: normal, control, positive control, YM with low, medium, high dosage each. All groups were prepared with femur fracture and treated diffrently. In order to measure bone regeneration effects, we analysed the levels of cyclooxygenase-2 (COX2), bone morphogenetic protein-2 (BMP2), collagen type Ⅱ alpha 1 chain (Col2a1), Sox9, runt-related transcription factor 2 (Runx2), and osterix genes expressed in bone. For morphological analysis, muscles were removed and femur was observed with naked eye. Results COX2 gene expression in bone marrow significantly decreased. BMP2 gene expression significantly increased. Col2a1 gene expression significantly increased. Sox9 gene expression increased as well. Runx2 gene expression in bone marrow increased, but there was no statistical significance. Osterix gene expression significantly increased. Union of the fracture site progressed more in YM group compared to the control group. The fracture union score was significantly decreased in YM group compared to the control group. Conclusions YM showed anti-inflammatory effect, promoted bone regeneration by stimulating the bone regeneration factor. In conclusion, YM can help fracture healing and it well be applied clinically to patients with fracture. (J Korean Med Rehabil 2020;30(4):17-30)

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