Anti-oxidation and Fat Accumulation Inhibitory Effects of Silbi-san (실비산의 항산화 및 지방축적 억제 효과)

Geon Woo Kim (김건우); Chang-Hoon Woo (우창훈); Kim Youngjun (김영준); Hee-Duk An (안희덕)

doi:10.18325/jkmr.2020.30.4.41

Anti-oxidation and Fat Accumulation Inhibitory Effects of Silbi-san

Journal of Korean Medicine Rehabilitation
Abbr : JKMR
2020, 30(4), pp.41-53
DOI : 10.18325/jkmr.2020.30.4.41
Publisher : The Korean Academy Of Oriental Rehabilitation Medicine
Research Area : Medicine and Pharmacy > Korean Medicine
Received : September 23, 2020
Accepted : October 22, 2020
Published : October 31, 2020

Geon Woo Kim ¹, Chang-Hoon Woo ², Kim Youngjun ², Hee-Duk An ²

¹대구한의대학교 대학원 한의학과 한방재활의학과학
²대구한의대학교 한의과대학 한방재활의학과교실

Accredited

ABSTRACT

Objectives The purpose of this study was to investigate the effects of Silbi-san on the antioxidant and fat accumulation inhibition and to analyze the anti-obesity effect by analyzing the changes in serum lipid composition in obese mice. Methods We compared contents of phytochemicals like total polyphenols and total flavonoid and antioxidant activities such as 2,2-dipheny-1-picrylhydrazyl and 2.2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging activity. After Silbi-san in 3T3-L1 cells in vitro and mouse adipose tissue ex vivo, we quantified intracellular triglyceride accumulation and lipolysis. Moreover, the anti-obesity activity though inhibiting pancreatic lipase were analyzed. In 3T3-L1 cells, morphological changes showed that control cells had many lipid while cells treated with Silbi-san had less lipid accumulation. 30% EtOH Silbi-san treatment also suppressed the fat absorption by inhibiting the activity of pancreatic lipase and led to high lipolysis through promoting glycerol release. The experimental group was divided into four groups: Normal group fed normal feed, Control group fed 60% high fat diet (HFD) and distilled water, drug group fed 60% high fat diet and 200 mg/kg of Silbi-san water extract, drug group fed 60% HFD and 200 mg/kg of Silbi-san 30% ethanol extract. Results Serum total cholesterol content and serum low density lipoprotein-cholesterol content were significantly decreased in the Silbi-san extract group compared to the control group, serum high density lipoprotein-cholesterol content was significantly increased in Silbi-san extract group. Conclusions In this study, the antioxidant and fat accumulation inhibitory effects of Silbi-san were confirmed. (J Korean Med Rehabil 2020;30(4):41-53)

KEYWORDS

Silbi-san, Antioxidants, Anti-obesity agents, Lipolysis, Pancrelipase

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Anti-oxidation and Fat Accumulation Inhibitory Effects of Silbi-san

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Anti-oxidation and Fat Accumulation Inhibitory Effects of Silbi-san

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