2016, Vol.16, No.2

Journal of Korean Medicine for Obesity Research 2023 KCI Impact Factor : 0.43

pISSN : 1976-9334 / eISSN : 2288-1522
https://journal.kci.go.kr/jkomor

Objectives: The purpose of this study was to observe the effects of Citri Reticulatae ViridePericarpium (CP) on 4-Hydroxynonenal (4-HNE)-induced inflammation in PC12 cells. Methods: 4-HNE was treated in PC12 cell to cause inflammatory response, and then treated withCP water extract at 25, 50, and 100 g/ml. The phosphorylation of Jun N-terminal kinase (JNK)and the expression of NF-B in PC12 cells were determined by Western blot, respectively. Results: The phosphorylation of JNK was significantly decreased in 4-HNE-stimulated PC12 cellby the treatment of CP extract at 25 g/ml. The 4-HNE-induced expression of nuclear factorkappa-light-chain-enhancer of activated B cells (NF-B) p65 in nuclear of the cells wassignificantly decreased in PC12 cell by treatment with CP extract at 25, 50, and 100 g/ml. Conclusions: These results suggest that CP water extract has an anti-inflammatory activitythrough suppressing the JNK and NF-B activation.

Objectives: This study investigated the effects of Scutellariae Radix extract (SRE) on lipids metabolism, oxidation and the production of pro-inflammatory cytokines in rats fed highly oxidized fat. Methods: To induce obesity, male Sprague‐Dawley rats were fed a highly oxidized fat diet for 10 weeks. SRE at 100 mg/kg were administered orally to obesity‐induced rats for 6 weeks, and their lipid metabolism, oxidation and production of pro-inflammatory cytokines were examined. Results: The concentrations of free fatty acid, triglyceride, total cholesterol, and low density lipoprotein-cholesterol in plasma decreased in SRE-treated groups, although the difference was not significant between control and SRE-treated groups, while that of high density lipoprotein- cholesterol significantly increased in SRE group. The concentrations of total cholesterol and triglyceride in the liver were tended to decrease in SRE-treated group. The concentrations of thiobarbituric acid in plasma and liver were lower in SRE group than in control group. The levels of glutamic oxaloacetic transaminase and glutamic pyruvic transaminase in plasma were decreased in SRE group. Activities of glutathione peroxidase, superoxide dismutase, and catalase in liver were tended to increase in the SRE group. The plasma concentrations of interleukin (IL)-1b, tumor necrosis factor (TNF)-a and IL-6 were lower in SRE group than in control group, while that of IL-10 was higher. The liver concentrations of IL-1b, TNF-a, and IL-6 were tended to decrease while that of IL-10 tended to increase in SRE group. Conclusions: Finally SRE could be used in the production of nutraceuticals for lowering lipids and exerting anti-oxidation and anti-inflammatory effects in obesity rats fed highly oxidized rat

Objectives: This study was designed to evaluate the anti-diabetic effects of Spargaium stoloniferum Buchanan-Hamilton (Sparganii Rhizoma, SR) extract on diabetic rats. Methods: Diabetes was induced with Sprague-Dawley rats by high fat/high sucrose (HF/HS) diet for 4 weeks and injection of a single low dose of streptozotocin (STZ; 35 mg/kg). SR water extract at 500 mg/kg was orally administrated once a day for 4 weeks. Body weights, food and water intakes and urine volumes were measured. The levels of glucose, insulin, total cholesterol, glutamic oxaloacetic transaminase and glutamic-pyruvic transaminase (GPT) were measured in the sera of rats. Histological changes were observed in pancreas, liver, and kidney tissues by H&E staining. Results: The administration of Sparganii Rhizoma extract at 500 mg/kg in diabetic rats did not shown a significant difference in body weight changes and GPT levels, but showed meaningful changes in an increase of urination volume, and decrease of serum glucose and insulin levels. Total cholesterol and GPT levels were also significantly decreased after SR extract administration in diabetic rats. Furthermore, the abnormal changes of pancreas, liver and kidney were also improved by Sparganii Rhizoma extract administration. Conclusions: These results indicate that SR extract can improve HF/HS-diet and STZ-induced diabetic damages in rats through inhibition of the blood glucose and insulin increase.