Purpose: The purposes of this study were to assess the knowledge, attitude, and practice (KAP) of standard precautions for infection control among healthcare workers (HCWs) in military division medical facilities, to investigate associations among the KAP, and to provide preliminary data for developing infection control education programs. Methods: This study is a cross-sectional, descriptive study using a self-reporting questionnaire. The subjects consisted of 141 HCWs including nursing officers, army surgeons, medical non-commissioned officers, and medics working at or below the military division medical facilities. The data were collected from August 14, to September 5, 2018 by visiting the facilities. Statistical methods including t-test, Mann-Whitney test, one-way ANOVA with Tukey post-hoc, or Kruskal-Wallis test were applied for the data analysis. Results: The knowledge scores according to general characteristics were significantly associated with sex, military rank, type of position. The practice scores according to general characteristics were significantly associated with total working experience. The results of the correlation analysis between the variables, knowledge and practice of standard precautions of infection control in nursing officer were found to be significant in this study (r=.46, p=.032). Conclusion: It is necessary to conduct further observational study that can monitor the actual practice of standard precautions of the subjects as well as the development of practical education programs for infection control in military division medical facilities.