Purpose: The purpose of this study was to assess the levels of emotional intelligence, interpersonal relationship, and self-leadership among junior nursing officers, and to examine their effects on job satisfaction. Methods: This study employed a cross-sectional survey design involving 79 junior nursing officers. The structured questionnaire included 77 items measuring the levels of emotional intelligence, interpersonal relationship, self-leadership, and job satisfaction. Results: The average scores of emotional intelligence, interpersonal relationship, self-leadership, and job satisfaction were 3.90±0.51, 3.94±0.40, 3.71±0.40, and 3.58±0.64 out of 5 points, respectively. Job satisfaction was positively correlated with emotional intelligence (r=.59, p<.001), interpersonal relationship (r=.50, p<.001), and self-leadership (r=.63, p<.001). The explanatory power of the regression model was 46.6%, and the model was statistically significant (F=18.02, p<.001). Also, the significant predictors of job satisfaction were self-leadership (β=.40, p<.001) and interpersonal relationship (β=.21, p=.044). Conclusion: In order to increase job satisfaction of junior nursing officers, it is necessary to develop a customized educational program that can improve self-leadership and interpersonal relationship, and incorporate them into The Officer Basic Course or continuing nursing education programs.