@article{ART001452470},
author={김학선 and 이광일 and 김향 and Un-Hye Kwon and Mi-Ran Nam and 장주웅 and In-Je Cho and Boram Kim and 이환모 and 문성환},
title={Tissue Engineering of the Intervertebral Disc with Cultured Nucleus Pulposus Cells Using Atelocollagen Scaffold and Gene Therapy},
journal={Journal of Korean Society of Spine Surgery},
issn={2093-4378},
year={2010},
volume={17},
number={2},
pages={49-56}
TY - JOUR
AU - 김학선
AU - 이광일
AU - 김향
AU - Un-Hye Kwon
AU - Mi-Ran Nam
AU - 장주웅
AU - In-Je Cho
AU - Boram Kim
AU - 이환모
AU - 문성환
TI - Tissue Engineering of the Intervertebral Disc with Cultured Nucleus Pulposus Cells Using Atelocollagen Scaffold and Gene Therapy
JO - Journal of Korean Society of Spine Surgery
PY - 2010
VL - 17
IS - 2
PB - Korean Society Of Spine Surgery
SP - 49
EP - 56
SN - 2093-4378
AB - Study Design: This is an in-vitro experiment using rabbit intervertebral disc (IVD) cells and growth factors.
Objectives: We wanted to determine the effect of types I,and II atelocollagen and growth factor gene therapy for matrix regeneration of rabbit IVD cells.
Summary of the Literature Review: Adenovirus-medicated growth factor gene therapy is efficient for matrix regeneration of the IVD. Atellocollagen has provided a favorable environment for matrix synthesis. However, a combined approach using gene and cell therapy in an atelocollagen scaffold has not yet been attempted.
Materials and Methods: Rabbit IVD cells were transduced with Ad/TGF-β1 and Ad/BMP-2. The cells were then implanted to the atelocollagen scaffold. The [methyl-3H]thymidine incorporation for DNA synthesis and the [35S]sulfur incorporation for proteoglycan synthesis were measured. RT-PCR was performed for assessing the aggrecan, collagen type I, collagen type II and osteocalcin mRNA expressions.
Results: The rabbit IVD cells with Ad/TGF-β1 and that were cultured in type I atelocollagen showed a 130% increase in new proteoglycan synthesis, while the rabbit IVD cells with Ad/TGF-β1 and that were cultured in type II atelocollagen showed a 180% increase in new proteoglycan synthesis (p<0.05). The rabbit IVD cells with Ad/BMP-2 and that were cultured in type I atelocollagen showed a 70% increase in new proteoglycan synthesis, while the rabbit IVD cells with Ad/BMP-2 and that were cultured in type II atelocollagen showed a 95% increase (p<0.05). Rabbit IVD cells with Ad/TGF-β1 and Ad/BMP-2 and that were cultured in type I and II atelocollagen demonstrated increased collagen type I and II mRNA expressions without an osteocalcin mRNA expression (p<0.05).
Conclusion: Cell and gene therapy in an atelocollagen scaffold provided a efficient mechanism for chondrogenic matrix regeneration of rabbit IVD cells.
KW - Intervertebral disc;Collagen;Scaffold;TGF-β1;BMP-2
DO -
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ER -
김학선, 이광일, 김향, Un-Hye Kwon, Mi-Ran Nam, 장주웅, In-Je Cho, Boram Kim, 이환모 and 문성환. (2010). Tissue Engineering of the Intervertebral Disc with Cultured Nucleus Pulposus Cells Using Atelocollagen Scaffold and Gene Therapy. Journal of Korean Society of Spine Surgery, 17(2), 49-56.
김학선, 이광일, 김향, Un-Hye Kwon, Mi-Ran Nam, 장주웅, In-Je Cho, Boram Kim, 이환모 and 문성환. 2010, "Tissue Engineering of the Intervertebral Disc with Cultured Nucleus Pulposus Cells Using Atelocollagen Scaffold and Gene Therapy", Journal of Korean Society of Spine Surgery, vol.17, no.2 pp.49-56.
김학선, 이광일, 김향, Un-Hye Kwon, Mi-Ran Nam, 장주웅, In-Je Cho, Boram Kim, 이환모, 문성환 "Tissue Engineering of the Intervertebral Disc with Cultured Nucleus Pulposus Cells Using Atelocollagen Scaffold and Gene Therapy" Journal of Korean Society of Spine Surgery 17.2 pp.49-56 (2010) : 49.
김학선, 이광일, 김향, Un-Hye Kwon, Mi-Ran Nam, 장주웅, In-Je Cho, Boram Kim, 이환모, 문성환. Tissue Engineering of the Intervertebral Disc with Cultured Nucleus Pulposus Cells Using Atelocollagen Scaffold and Gene Therapy. 2010; 17(2), 49-56.
김학선, 이광일, 김향, Un-Hye Kwon, Mi-Ran Nam, 장주웅, In-Je Cho, Boram Kim, 이환모 and 문성환. "Tissue Engineering of the Intervertebral Disc with Cultured Nucleus Pulposus Cells Using Atelocollagen Scaffold and Gene Therapy" Journal of Korean Society of Spine Surgery 17, no.2 (2010) : 49-56.
김학선; 이광일; 김향; Un-Hye Kwon; Mi-Ran Nam; 장주웅; In-Je Cho; Boram Kim; 이환모; 문성환. Tissue Engineering of the Intervertebral Disc with Cultured Nucleus Pulposus Cells Using Atelocollagen Scaffold and Gene Therapy. Journal of Korean Society of Spine Surgery, 17(2), 49-56.
김학선; 이광일; 김향; Un-Hye Kwon; Mi-Ran Nam; 장주웅; In-Je Cho; Boram Kim; 이환모; 문성환. Tissue Engineering of the Intervertebral Disc with Cultured Nucleus Pulposus Cells Using Atelocollagen Scaffold and Gene Therapy. Journal of Korean Society of Spine Surgery. 2010; 17(2) 49-56.
김학선, 이광일, 김향, Un-Hye Kwon, Mi-Ran Nam, 장주웅, In-Je Cho, Boram Kim, 이환모, 문성환. Tissue Engineering of the Intervertebral Disc with Cultured Nucleus Pulposus Cells Using Atelocollagen Scaffold and Gene Therapy. 2010; 17(2), 49-56.
김학선, 이광일, 김향, Un-Hye Kwon, Mi-Ran Nam, 장주웅, In-Je Cho, Boram Kim, 이환모 and 문성환. "Tissue Engineering of the Intervertebral Disc with Cultured Nucleus Pulposus Cells Using Atelocollagen Scaffold and Gene Therapy" Journal of Korean Society of Spine Surgery 17, no.2 (2010) : 49-56.