Control of Molecular Weight and Terminal Groups of Poly (3-hydroxybutyrate) in Bio-synthesis (미생물 합성에 의한 poly(3-hydroxybutyrate)의 분자량과말단관능기 제어)

Chan Woo Lee (이찬우)

doi:10.5764/TCF.2018.30.2.130

Control of Molecular Weight and Terminal Groups of Poly (3-hydroxybutyrate) in Bio-synthesis

Textile Coloration and Finishing
Abbr : Text. Color. and Finish.
2018, 30(2), pp.130-140
DOI : 10.5764/TCF.2018.30.2.130
Publisher : The Korean Society Of Dyers And Finishers
Research Area : Engineering > Fiber Engineering > Dyeing Engineering
Received : March 27, 2018
Accepted : June 7, 2018
Published : June 27, 2018

Chan Woo Lee ¹

¹호서대학교 바이오산업학부

Accredited

ABSTRACT

In the bio-synthesis of poly(3-hydroxybutyrate)(PHB), which is a kind of poly(3-hydroxyalkanoate)(PHA), aimed to control the low molecular weight of PHB and obtain a telechelic PHB. As a result of incubation of R. eutropha at 30℃ with ethylene glycol added as a chain transfer agent, PHB content on the dry cell weight increased up to 24h, however, it decreased after that, and the molecular weight of PHB increased from 9h to 12h, and then, decreased up to 72h. The decrease of the content and the molecular weight of PHB indicates that PHB was decomposed as an energy source in bacterial cells and was incorporated into metabolic pathways. 1H-NMR of the obtained PHB after incubation for 72h was measured to determine the terminal groups of the PHB during incubation. As the results of １H-NMR measurement, the peaks derived from ethylene glycol in both terminals of PHB were observed. Which indicate that the terminal reaction was caused by the addition of ethylene glycol, and that telechelic PHB having hydroxyl group at the both terminals where molecular weight was controlled was successfully synthesized.

KEYWORDS

bio-synthesis, low molecular weight, terminal groups, telechelic, poly(3-hydroxybutyrate), energy source, chain transfer agent

Citation status

* References for papers published after 2023 are currently being built.

[journal] G. Q. Chen / 2009 / AMicrobial Polyhydroxyalkanoates(PHA)Based Bio-and Materials Industry / Chem. Soc. Rev 38(8) : 2434~

[journal] S. Y. Dai / 2008 / Enzymatic Preparation of Novel ThermoplasticDi-blockCopolyestersContainingPoly[(R)-3-hydroxybutyrate] and Poly(epsilon-caprolactone)Blocks viaRing-opening Polymerization / Biomacromolecules 9(7) : 1883~

[journal] S. Fiedler / 2000 / Pha Gmediated Synthesis of Poly(3-hydroxyalkanoates) Consisting of Medium-chain-length Constituents from Nonrelated Carbon Sources in Recombinant Pseudomonas Fagi / Appl. Environ. Microbiol. 66(5) : 2117~

[journal] C. S. Ha / 2002 / Miscibility, Properties, and Biodegradability of Microbial Polyester Containing Blends / Prog. Polym. Sci. 27(4) : 759~

[journal] S. K. Misra / 2006 / Polyhydroxyalkanoate(PHA)/inorganic Phase Composites for Tissue Engineering Applications / Biomacromolecules 7(8) : 2249~

[journal] F. Ravenelle / 2002 / One-step Synthesis of Amphiphilic Diblock Copolymers from Bacterial Poly ([R]-3-hydroxybutyric acid) / Biomacromolecules 3(5) : 1057~

[journal] A. Romano / 2005 / Expression of Poly-3-(R)-hydroxyalkanoate( PHA) Polymerase and Acyl-CoA-transacylase in Plastids of Transgenic Potat Leads to the Synthesis of a Hydrophobic Polymer, Presumably Medium-chain-length PHAs / Planta 220(3) : 455~

[journal] Y. Saito / 1996 / Microbial Synthesis and Properties of Poly(3-hydroxybutyrate-co-4-hydroxybutyrate): PHB and Hydroxyalkanoate Polymers / Polym. Int. 39(3) : 169~

[journal] X. W. Shen / 2009 / Production and Characterization of Homopolymer Poly (3-hydroxyvalerate) (PHV) Accumulated by Wild Type and Recombinant Aeromonas Hydrophila Strain 4AK4 / Bioresour Technol 100(18) : 4296~

[journal] K. Yamanaka / 2009 / End-Group Analysis of Bacterially Produced Poly(3-hydroxybutyrate): Discovery of Succinate as the Polymerization Starter / Macromolecules 42(12) : 4038~

[journal] Y. Kawaguchi / 1992 / Kinetics and Mechanism of Synthesis and Degradation of Poly (3-hydroxybutyrate) in Alcaligenes Eutrophus / Macromolecules 25(9) : 2324~

[journal] Y. Jia / 2001 / Mechanistic Studies on Class I Polyhydroxybutyrate( PHB) Synthase from Ralstonia eutropha: Class I and III Synthases Share a Similar catalytic Mechanism / Biochemistry 40(4) : 1011~

[journal] S. Zhang / 2003 / Mechanism of the Polymerization Reaction Initiated and Catalyzed by the Polyhydroxybutyrate Synthase of Ralstonia eutropha / Biomacromolecules 4(3) : 504~

[journal] R. J. Griebel / 1971 / Metabolism of Poly-β-Hydroxy butyrate: Effect of Mild Alkaline Extraction on Native Poly-β-Hydroxy butyrate Granules / J. Bacteriol 108(2) : 782~

[journal] L. L. Madison / 1999 / Metabolic Engineering of Poly(3-hydroxyalkanoates): from DNA to Plastic / Microbiol. Mol. Biol. Rev. 63(1) : 21~

[journal] A. Steinbuchel / 1998 / Bacterial and Other Biological Systems for Polyester Production / Trends Biotechnol. 16(10) : 419~

[journal] Y. Doi / 1986 / Proton and Carbon 13NMR Analysis of Poly(β-hydroxybutyrate) Isolated from Bacillus megaterium / Macromolecules 19(4) : 1274~

[journal] Y. Doi / 1986 / Nuclear Magnetic Resonance Studies on Poly(β-hydroxybutyrate) and a Copolyester of β-hydroxybutyrate and β-hydroxyvalerate Isolated from Alcaligenes eutrophus H16 / Macromolecules 19(11) : 2860~

[journal] G. Yu / 2000 / Characterization of Low Molecular Weight Poly(β-hydroxybutyrate)s from Alkaline and Acid Hydrolysis / Polymer 41(3) : 1087~

[journal] S. Nguyen / 2002 / Thermal Degradation of Poly(3-hydroxyalkanoates): Preparation of Well-Defined Oligomers / Biomacromolecules 3(1) : 219~

[journal] G. Adamus / 2007 / Structural Analysis of Poly[(R,S)-3-hydroxybutyrate-co-L-lactide] Copolyesters by Electrospray Ionization Ion Trap Mass Spectrometry / Rapid Commun. Mass Spectrom. 21(15) : 2477~

[journal] S. Carroccio / 2004 / MALDI Investigation of Photooxidation in Aliphatic Polyesters: Poly(butylene succinate) / Macromolecules 37(17) : 6576~

[journal] P. Rizzarelli / 2006 / Matrix-assisted Laser Desorption/ionization Time-of-flight/time-of-flight Tandem Mass Spectra of Poly(butylene adipate) / Rapid Commun. Mass Spectrom. 20(11) : 1683~

[journal] M. Kawalec / 2007 / Carboxylate Induced Degradation of Poly(3-hydroxybutyrate)s / Biomacromolecules 8(4) : 1053~

This paper was written with support from the National Research Foundation of Korea.

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Control of Molecular Weight and Terminal Groups of Poly (3-hydroxybutyrate) in Bio-synthesis

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Control of Molecular Weight and Terminal Groups of Poly (3-hydroxybutyrate) in Bio-synthesis

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