Induction of Myogenic Differentiation in Myoblasts by Electrical Stimulation

Hyeonjeong, Je (제현정); Kim Min Gu (김민구); Cho, Il-Hoon (조일훈); Hyuck Joon KWON (권혁준)

doi:10.13066/kspm.2019.14.2.63

Induction of Myogenic Differentiation in Myoblasts by Electrical Stimulation

Journal of the Korean Society of Physical Medicine
Abbr : J Korean Soc Phys Med
2019, 14(2), pp.63-70
DOI : 10.13066/kspm.2019.14.2.63
Publisher : The Korean Society of Physical Medicine
Research Area : Medicine and Pharmacy > Physical Therapy > Other physical therapy
Received : April 4, 2019
Accepted : April 22, 2019
Published : May 31, 2019

Hyeonjeong, Je ¹, Kim Min Gu ¹, Cho, Il-Hoon ², Hyuck Joon KWON ²

¹을지대학교(성남캠퍼스)
²을지대학교

Accredited

ABSTRACT

PURPOSE: While electrical stimulation (ES) is known to be a safe and flexible tool in rehabilitation therapy, it has had limited adoption in muscle regeneration. This study was performed to investigate whether ES can induce myogenic differentiation and to clarify the mechanism underlying the effects of ES on myogenic differentiation. METHODS: This study used rat L6 cell lines as myoblasts for myogenic differentiation. Electric stimulation was applied to the cells using a C-Pace EP culture pacer (IonOptix, Westwood, Ma, USA). The gene expressions of myogenic markers were examined using qPCR and immunochemistry. RESULTS: Our study showed that ES increased the thickness and length of myotubes during myogenic differentiation. It was found that ES increased the expression of myogenic markers, such as MyoD and Myogenin, and also activated the fusion of the myoblast cells. In addition, ES suppressed the expression of small GTPases, which can explain why ES promotes myogenic differentiation. CONCLUSION: We found that ES induced myogenic differentiation by suppressing small GTPases, inhibiting cell division. We suggest that ES-based therapies can contribute to the development of safe and efficient muscle regeneration.

KEYWORDS

Electrical stimulations, Myoblast, Myogenesis, Small GTPase

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This paper was written with support from the National Research Foundation of Korea.

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Journal of the Korean Society of Physical Medicine 2023 KCI Impact Factor : 0.45

Induction of Myogenic Differentiation in Myoblasts by Electrical Stimulation

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* References for papers published after 2023 are currently being built.

Journal of the Korean Society of Physical Medicine 2023 KCI Impact Factor : 0.45

Induction of Myogenic Differentiation in Myoblasts by Electrical Stimulation

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