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Anti-inflammatory Activities of Herbal Formulas for Sasang Constitutional Medicine

  • Journal of Sasang Constitution and Immune Medicine
  • Abbr : J Sasang Constitut Med
  • 2010, 22(4), pp.56-64
  • Publisher : The Society of Sasang Constitution and Immune Medicine
  • Research Area : Medicine and Pharmacy > Korean Medicine

이진아 1 하혜경 1 이호영 1 정다영 1 이준경 1 황대선 1 신현규 1

1한국한의학연구원 표준화연구본부 한약EBM연구센터

Accredited

ABSTRACT

1. Objectives 4 herbal formulas (Yanggyeoksanhwa-tang, Yeoldahanso-tang, Cheongsimyeonja-tang and Taeeumjowi-tang) were applied to investigate the anti-inflammatory activities. In many studies, plant-derived anti-inflammatory efficacies have been investigate for their potential inhibitory effects on lipopolysaccharide (LPS)-stimulated macrophages. This study was performed to examine the anti-inflammatory activities of 4 herbal formulas on LPS-stimulated RAW 264.7 cells. 2. Methods The productions of nitric oxide (NO), prostaglandin (PG)E2, interleukin(IL)-6 and tumor necrosis factor (TNF)-α were examined in the presence of the 4 herbal formulas in RAW 264.7 cells. The cells were incubated with LPS 1 μg/mL and 4 herbal formulas for 18 hrs. The anti-inflammatory activity of 4 herbal formulas were investigate by carrageenin-induced paw edema in rats. The paw volume was measured at 2 and 4 hrs following carrageenin- induced paw edema in rats. 3. Results Yanggyeoksanhwa-tang and Cheongsimyeonja-tang showed inhibitory effect on PGE2 production in LPS-stimulated RAW 264.7 cells and a reduction in carrageenin-induced paw edema on rats. Yanggyeoksanhwa-tang showed inhibi- tory effect on IL-6 in LPS-stimulated RAW 264.7 cells. 4 herbal formulas not affect on NO and TNF-α inhibition in LPS-stimulated RAW 264.7 cells. 4. Conclusions These results suggested that Yanggyeoksanhwa-tang and Cheongsimyeonja-tang have anti-inflammatory activity.

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