Simultaneous Analysis and Anti-obesity Effect of Taeeumin Cheongsimyeonja-tang (太陰人 淸心蓮子湯의 동시분석 및 항비만 효과)

ChangSeob Seo (서창섭); Soo-Jin Jeong (정수진); 김정훈; Sae-Rom Yoo (유새롬); 신현규

doi:10.7730/JSCM.2013.25.1.51

Simultaneous Analysis and Anti-obesity Effect of Taeeumin Cheongsimyeonja-tang

Journal of Sasang Constitutional Medicine
Abbr : J Sasang Constitut Med
2013, 25(1), pp.51-61
DOI : 10.7730/JSCM.2013.25.1.51
Publisher : The Society Of Sasang Constitutional Medicine
Research Area : Medicine and Pharmacy > Korean Medicine

ChangSeob Seo ¹, Soo-Jin Jeong ¹, 김정훈 ¹, Sae-Rom Yoo ¹, 신현규 ¹

¹한국한의학연구원

Accredited

ABSTRACT

Objectives We performed the simultaneous analysis for three compounds in Cheongsimyeonja-tang (CSYJT) and evaluated its anti-obesity effect. Methods The column for separation of three compounds was used Gemini C18 column and maintained at 40℃. The mobile phase for gradient elution consisted of two solvent systems. The analysis was carried out at a flow rate of 1.0 mL/min with PDA detection at 275 nm. The injection volume was 10 μL. 3T3-L1 preadipocytes were differentiated into adipocytes by adding insulin, dexamethasone and 3-isobutyl-1-methylxanthine (IBMX) for 8 days in the absence or presence of CSYJT. Anti-obesity effects of CSYJT were evaluated by Oil Red O staining, glycerol-3-phosphate dehydrogenase (GPDH) activity, triglyceride contents, and leptin production. Results Calibration curves were acquired with r2>0.9999. The contents of baicalin, wogonoside and baicalein in CSYJT were 14.54-14.65 mg/g, 5.24-5.27 mg/g and 0.01-0.02 mg/g, respectively. CSYJT showed inhibitory effect on lipid accumulation and GPDH activity in the differentiated 3T3-L1 cells. Furthermore, CSYJT significantly decreased contents of triglyceride and leptin production in 3T3-L1 adipocytes. Conclusions These results will be helpful to improve quality control and anti-obesity effect of Taeeumin CSYJT.

KEYWORDS

Cheongsimyeonja-tang, HPLC-PDA, simultaneous analysis, obesity, 3T3-L1 adipocyte

Citation status

* References for papers published after 2023 are currently being built.

[journal] 박영신 / 2004 / Trend of Obesity in School Age Children in Seoul Over the Past 23 Years / Korean Journal of Pediatrics / 대한소아과학회 47(3) : 247~257

[journal] 허행전 / 2011 / Effect of Silybin on Body Weight and Glucose Tolerance in High-fat-diet Induced Obese Mice / KSBB Journal / 한국생물공학회 26(1) : 78~82

[journal] 신인순 / 2012 / The Effect of Natural Mixture Supplementation on Histopathological and Histomorphometrical aspects in High Fat Diet-induced Obese Mice / 대한본초학회지 / 대한본초학회 27(4) : 53~58

[journal] Reddy P / 1998 / Focus on orlistat: A nonsystemic inhibitor of gastrointestinal lipase for weight reduction in the management of obesity / Formulary 33 : 943~959

[journal] Peter C / 2001 / Drug treatment of obesity: from past failures to future successes / Br J Clin Pharmacol 51 : 13~25

[journal] Choussein S / 2009 / Daskalopoulou SS. Effect of antiobesity medications in patients with type 2 diabetes mellitus / Diabetes Obes Metab 11 : 641~664

[journal] 차민경 / 2006 / Effects of Bangpoongtongsungsan on adipose tissue and hyperlipidemia of 3T3-L1 induced Rats / 대한한방소아과학회지 / 대한한방소아과학회 20(2) : 177~196

[journal] 황상준 / 2006 / The inhibitory effects of Bangpungtongseoung-san on the obese gene and obded inhibitory about obede-mouse induced by high fat diet / 대한한의학회지 / 대한한의학회 27(1) : 11~22

[book] Lee JM / 1992 / Dongeuisusebowon / Yeogang Publishing Co : 271~272

[journal] Hwang SY / 2002 / Effects of cheongsimyeonja-tang water extract on the cultured primary hippocampal cell damagee by XO/HX / J Sasang Constitut Med 14(3) : 132~145

[journal] Kim HS / 2003 / A study of the effcets of Cheongsimyeonja-tang (CYT) on ECG in comparing before and after CYT medication: Case study of hospitalized Taeumin patients / J Sasang Constitut Med 15(3) : 147~157

[journal] 임종필 / 2007 / The Anti-oxidative and Immune-regulatory Effectof Chungsimyeonja-tang in Aged Rat / 사상체질의학회지 / 사상체질의학회 19(3) : 227~241

[journal] Park SC / 2003 / Effects CheongsimyeonJa-tang(CSYJT) on control of immune-function in highly purified mouse B cells and mast cell / J Sasang Constitut Med 15(2) : 166~179

[journal] Hong SC / 1995 / An experimental study on the anti-stress effect by Taeumin Chongsimyonjatang / J Sasang Constitut Med 7(2) : 227~240

[journal] Ju LS / 2004 / Study of Cheongsimyeonja-tang that get weight, hematology, biochemistry change by Wistar rat’s aging / J Daejeon Orient Med 13(2) : 337~345

[journal] 양가은 / 2011 / Literature Review of Studies of Obesity Using Tae-eumin Prescriptions and Herbs - Based on Korean Literature - / 대한한의학회지 / 대한한의학회 32(5) : 12~24

[journal] 최은주 / 2010 / The Suggestion on the Herbal Bathing and the Pinda Manual Therapy with Herbs for Obesity Patients according to Sasang Constitutions. / 한방비만학회지 / 한방비만학회 10(2) : 41~49

[journal] Reed BC / 1977 / Alterations in insulin binding accompanying differentiation of 3T3-L1 preadipocytes / Proc Natl Acad Sci USA 74(11) : 4876~4880

[journal] Frayn KN / 2003 / Integrative physiology of human adipose tissue / Int J Obes Relat Metab Disord 27(8) : 875~888

[journal] Klok MD / 2007 / The role of leptin and ghrelin in the regulation of food intake and body weight in humans: a review / Obes Rev 8(1) : 21~34

[journal] Shar NR / 2012 / Measuring adiposity in patients: the utility of body mass index (BMI), percent body fat, and leptin / PLoS One 7(4) : e33308~

[journal] Blum WF / 1997 / Plasma leptin levels in healthy children and adolescents: dependence on body mass index, body fat mass, gender, pubertal stage, and testosterone / J Clin Endocrinol Metab 82(9) : 2904~2910

[journal] Banks WA / 2004 / Moinuddin A, Shultz JM, Nakaoke R et al. Triglycerides induce leptin resistance at the blood-brain barrier / Diabetes 53(5) : 1253~1260

KJCKorea
Journal Central

Journal of Sasang Constitutional Medicine 2023 KCI Impact Factor : 0.42

Simultaneous Analysis and Anti-obesity Effect of Taeeumin Cheongsimyeonja-tang

ABSTRACT

KEYWORDS

Citation status

* References for papers published after 2023 are currently being built.

Journal of Sasang Constitutional Medicine 2023 KCI Impact Factor : 0.42

Simultaneous Analysis and Anti-obesity Effect of Taeeumin Cheongsimyeonja-tang

ABSTRACT

KEYWORDS

Statistics

Tools

Issue List

Citation status

KCI Citation Counts (5)

REFERENCES (23) * References for papers published after 2023 are currently being built.

Search PDF

Citation

* References for papers published after 2023 are currently being built.