Autophagy inducing Effect of modified Yeoldahanso-tang and its related Proteins in SH-SY5Y cells (熱多寒少湯 加減方의 自家貪食 유도 활성과 관련 단백질 탐색)

김희주; Nayoung， Bae (배나영); 장문희; Yang， Hyun Ok (양현옥); TaekWon ahn (안택원)

doi:10.7730/JSCM.2013.25.3.208

Autophagy inducing Effect of modified Yeoldahanso-tang and its related Proteins in SH-SY5Y cells

Journal of Sasang Constitution and Immune Medicine
Abbr : J Sasang Constitut Med
2013, 25(3), pp.208-217
DOI : 10.7730/JSCM.2013.25.3.208
Publisher : The Society of Sasang Constitution and Immune Medicine
Research Area : Medicine and Pharmacy > Korean Medicine

김희주 ¹, Nayoung， Bae ², 장문희 ³, Yang， Hyun Ok ¹, TaekWon ahn ⁴

¹한국과학기술연구원
²부산대학교
³대전대학교 한의과대학 사상체질과
⁴대전대학교

Accredited

ABSTRACT

Objectives Modified Yeolda-Hanso tang (MYH) is a traditional herbal formula in Korea for various diseases. MYH is containing the 10 herbs : Pueraria lobata (Willd.) Ohwi, Angelica tenuissima Nakai, Scutellaria baicalensis Georgi, Platycodon grandiflorum (Jacq), Angelicae Dahurica, Cimicifuga heracleifolia Kom, Raphanus sativa L., Polygala tenuifolia (Willd), Acorus gramineus Soland and Dimocarpus longan Lour. The 10 herbs is constituted as a ratio of the 6:4:2:1:2:2:2:4:6:6. We investigated neuroprotective effects of MYH on human neuroblastoma SH-SY5Y cells and evaluated the ability of MYH to prevent and treat for neurodegenerative diseases such as Parkinson’s disease via basal autophagy enhancement. Methods Pharmacological induction of Autophagy by MYH in SH-SY5Y cells:Induction of autophagy by MYH in human neuroblastoma SH-SY5Y cells was carreid out by immunoblot analysis with several autophagy markers. SH-SY5Y cells were treated with MYH at the concentration of 400 and 800 µg/ml for 24 hr. Specifically, the autophagosome proteins LC3 II and Atg5 levels were increased and autophagy pathway related proteins such as beclin-1, PI3 Kinase class III protein, ULK1, mTOR and AMPK were activated. Conclusions MYH can enhance the induction of autophagy through key regulator AMPK, mTOR, and Beclin-1 and it should be considered as a possible candidate of neuroprotective agents for such as Parkinson’s disease.

KEYWORDS

Modified Yeolda-Hanso tang (MYH), Neuroprotection, Parkinson’s disease, Autophagy

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[journal] Pan T / 2008 / Neuroprotection of rapamycin in lactacystin-induced neurodegeneration via autophagy enhancement / Neurobiology of Disease 32 : 16~25

[journal] 배나영 / 2009 / Protection effect of New-Yeolda-Hanso tang against β-Amyloid Induced Cytotoxicity in NGF-differentiated PC12 Cells / 사상체질의학회지 / 사상체질의학회 21(3) : 138~153

[journal] Bae NY / 2011 / The neuroprotective effect of modified Yeoldahanso-tang via autophagy enhancement in models of Parkinson’s disease / Journal of Ethnopharmacology 134 : 313~322

[journal] Dauer W / 2003 / Parkinson’s disease: mechanisms and models / Neuron 39 : 889~909

[journal] Pan T / 2008 / The role of autophagy-lysosome pathway in neurodegeneration associated with Parkinson’s disease / Brain 131 : 1969~1978

[journal] 이진아 / 2012 / Neuronal Autophagy: A Housekeeper or a Fighter in Neuronal Cell Survival? / Experimental Neurobiology / 한국뇌신경과학회 21(1) : 1~8

[journal] Hara T / 2006 / Suppression of basal autophagy in neural cells causes neurodegenerative disease in mice / Nature 441 : 885~889

[journal] Pan T / 2009 / Rapamycin protects against rotenone-induced apoptosis through autophagy induction / Neuroscience 164 : 541~551

[book] / 2004 / National federation of department of sasang constitutional medicine, colleges of oriental medicine, In Sasang constitutional medicine / Jipmoondang : 419~421

[journal] Song IB / 1996 / The Clinical Study on the Effect of Sasang Constitutional Medical Theraphy for Stroke / J Sasang Constitut Med 8(2) : 117~130

[journal] Eisenberg-Lerner A / 2009 / Life and death partners : apoptosis, autophagy and the cross-talk between them / Cell Death and Differentiation 16 : 966~975

[journal] Mauthe M / 2011 / Resveratrol-mediated autophagy requires WIPI-1-regulated LC3 lipidation in the absence of induced phagophore formation / Autophagy : 1448~1461

[journal] Underwood B. R / 2010 / Antioxidants can inhibit basal autophagy and enhance neurodegeneration in models of polyglutamine disease / Human Molecular Genetics 17 : 3413~3429

[journal] Kim HS / 2009 / Activation of autophagy during glutamate-induced HT22 cell death / Biochemical and Biophysical Research Communications 388 : 339~344

[journal] Shin HY / 2011 / mTOR Inhibitor as a Potential Drug of Age-related Disease / Korean J Clin Geri 12 : 149~159

[journal] Liang J / 2007 / The energy sensing LKB1-AMPK pathway regulates p27(kip1) phosphorylation mediating the decision to enter autophagy or apoptosis / Nat Cell Biol 9 : 218~224

[journal] Williams T / 2009 / Basal autophagy induction without AMP-activated protein kinase under low glucose conditions / Autophagy 5 : 1155~1165

[journal] Wu Y / 2011 / Resveratrol-Activated AMPK/SIRT1/Autophagy in Cellular Models of Parkinson’s Disease / Neurosignals 19 : 163~174

[journal] Hay N / 2004 / Upstream and downstream of mTOR / Genes Dev 18 : 1926~1945

[journal] Slomovitz B. M / 2012 / The PI3K/AKT/mTOR pathway as a therapeutic target in endometrial cancer / Clin Cancer Res 21 : 5856~5864

[other] Jaclyn L. P / 2008 / Targeting the PI3K/Akt/mTOR pathway: Effective combinations and clinical considerations / Drug Resistance Updates : 32~50

[report] Sebastian A / 2011 / Role of AMPK-mTOR-Ulk1/2 in the Regulation of Autophagy: Cross Talk, Shortcuts, and Feedbacks / MCB : 06159~06111

[journal] Qin L / 2010 / ER stress negatively regulates AKT/TSC/mTOR pathway to enhance autophagy / Autophagy (2) : 239~247

[journal] Pan T / 2009 / Rapamycin protects against rotenone-induced apoptosis through autophagy induction / Neuroscience 164 : 541~551

[journal] Wang R. C / 2012 / Akt-mediated regulation of autophagy and tumorigenesis through Beclin 1 phosphorylation / Science 338(6109) : 956~959

[journal] Ralph A / 2011 / Autophagy failure in Alzheimer’s disease—locating the primary defect / Neurobiology of Disease 43 : 38~45

[journal] Alirezaei M / 1998 / Short-term fasting induces profound neuronal autophagy / Autophagy 6 : 702~710

[journal] Dadakhujaev S / 2010 / Autophagy protects the rotenone-induced cell death in alpha-synuclein overexpressing SH-SY5Y cells / Neuroscience Letters 472 : 47~52

[journal] Wilms H / 2007 / Imflammation in Parkinson’s diseases and other neurodegenerative diseases: cause and therapeutic implications / Curr Pharm Des 13 : 1925~1928

[journal] Ravikumar B / 2008 / Rapamycin pre-treatment protects against apoptosis / Human Molecular Genetics 7 : 1209~1216

[web] / Cell signaling Technology / http://www.cellsignal.com/pathways/autophagy-signaling.jsp

This paper was written with support from the National Research Foundation of Korea.

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Journal of Sasang Constitution and Immune Medicine 2023 KCI Impact Factor : 0.42

Autophagy inducing Effect of modified Yeoldahanso-tang and its related Proteins in SH-SY5Y cells

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* References for papers published after 2023 are currently being built.

Journal of Sasang Constitution and Immune Medicine 2023 KCI Impact Factor : 0.42

Autophagy inducing Effect of modified Yeoldahanso-tang and its related Proteins in SH-SY5Y cells

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