KJCKorea
Journal Central

@article{ART002694961},
author={Dinç Bircan and Ünlü Ayhan and Bektaş Muhammet},
title={Characterization of short-length multi-walled carbon nanotubes and cytotoxicity on MDA-MB-231 and HUVEC cell lines},
journal={Carbon Letters},
issn={1976-4251},
year={2020},
volume={30},
number={2},
pages={143-153},
doi={10.1007/s42823-019-00081-5}

TY - JOUR
AU - Dinç Bircan
AU - Ünlü Ayhan
AU - Bektaş Muhammet
TI - Characterization of short-length multi-walled carbon nanotubes and cytotoxicity on MDA-MB-231 and HUVEC cell lines
JO - Carbon Letters
PY - 2020
VL - 30
IS - 2
PB - Korean Carbon Society
SP - 143
EP - 153
SN - 1976-4251
AB - Multi-walled carbon nanotubes (MWNTs) are suitable for delivering large biomolecules with lower cytotoxicity values and low prime cost. Surface modifcations of MWNTs afect interaction with cells and proteins. Oxidation with strong acids decreases cytotoxicity of CNTs and increases protein-loading capacity. Here, after oxidation, TEM images revealed more aligned structure and carboxylated groups at the surface which decreases toxicity. Functionalized MWNTs showed more gradual degradation than the pristine MWNTs and mass loss increased by 2% in the same temperature range. Raman spectroscopy corrected graphitic structure with characteristic D and G bands at 1330 and 1579 cm−1 and increased intensity after oxidation. FTIR spectroscopy peaks at 1443 cm−1, 1560, 1640 cm−1, 2100–2200 cm−1 and 3426 cm−1 are ascribed to C–O–C vibrational stretch, C=C bonds, vibration of C≡C bonds and stretch of hydroxyl groups, respectively. The sonication�driven dispersion of in phosphate-bufered saline, distilled water and cell culture medium were detected by UV–vis–NIR spectroscopy, water-dispersed functionalized MWNTs revealed the highest absorbance value. Cytotoxicity of MWNTs was investigated before and after functionalization in breast cancer (MDA-MB-231) and human vein endothelial (HUVEC) cells. Relatively low-toxicity results were obtained in functionalized MWNTs and cellular uptake of MWNTs were corrected with fuorescent imaging of cells and cell lysates. Protein-loading capacity of fsMWNTs (functionalized short-length multi-walled carbon nanotubes) was evaluated by using bovine serum albumin (BSA) and with an equal amount of fsMWNTs and BSA; 36% binding yield was obtained. Protein corona after covalent functionalization potentially lowered cytotoxicity up to 6%
KW - Multi-walled carbon nanotubes · Characterization · Bovine serum albumin · Cytotoxicity · Solubility
DO - 10.1007/s42823-019-00081-5
ER -

Dinç Bircan, Ünlü Ayhan and Bektaş Muhammet. (2020). Characterization of short-length multi-walled carbon nanotubes and cytotoxicity on MDA-MB-231 and HUVEC cell lines. Carbon Letters, 30(2), 143-153.

Dinç Bircan, Ünlü Ayhan and Bektaş Muhammet. 2020, "Characterization of short-length multi-walled carbon nanotubes and cytotoxicity on MDA-MB-231 and HUVEC cell lines", Carbon Letters, vol.30, no.2 pp.143-153. Available from: doi:10.1007/s42823-019-00081-5

Dinç Bircan, Ünlü Ayhan, Bektaş Muhammet "Characterization of short-length multi-walled carbon nanotubes and cytotoxicity on MDA-MB-231 and HUVEC cell lines" Carbon Letters 30.2 pp.143-153 (2020) : 143.

Dinç Bircan, Ünlü Ayhan, Bektaş Muhammet. Characterization of short-length multi-walled carbon nanotubes and cytotoxicity on MDA-MB-231 and HUVEC cell lines. 2020; 30(2), 143-153. Available from: doi:10.1007/s42823-019-00081-5

Dinç Bircan, Ünlü Ayhan and Bektaş Muhammet. "Characterization of short-length multi-walled carbon nanotubes and cytotoxicity on MDA-MB-231 and HUVEC cell lines" Carbon Letters 30, no.2 (2020) : 143-153.doi: 10.1007/s42823-019-00081-5

Dinç Bircan; Ünlü Ayhan; Bektaş Muhammet. Characterization of short-length multi-walled carbon nanotubes and cytotoxicity on MDA-MB-231 and HUVEC cell lines. Carbon Letters, 30(2), 143-153. doi: 10.1007/s42823-019-00081-5

Dinç Bircan; Ünlü Ayhan; Bektaş Muhammet. Characterization of short-length multi-walled carbon nanotubes and cytotoxicity on MDA-MB-231 and HUVEC cell lines. Carbon Letters. 2020; 30(2) 143-153. doi: 10.1007/s42823-019-00081-5

Dinç Bircan, Ünlü Ayhan, Bektaş Muhammet. Characterization of short-length multi-walled carbon nanotubes and cytotoxicity on MDA-MB-231 and HUVEC cell lines. 2020; 30(2), 143-153. Available from: doi:10.1007/s42823-019-00081-5

Dinç Bircan, Ünlü Ayhan and Bektaş Muhammet. "Characterization of short-length multi-walled carbon nanotubes and cytotoxicity on MDA-MB-231 and HUVEC cell lines" Carbon Letters 30, no.2 (2020) : 143-153.doi: 10.1007/s42823-019-00081-5