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Modified screen-printed electrochemical biosensor design compatible with mobile phones for detection of miR-141 used to pancreatic cancer biomarker

  • Carbon Letters
  • Abbr : Carbon Lett.
  • 2023, 33(6), pp.1863-1873
  • DOI : 10.1007/s42823-023-00545-9
  • Publisher : Korean Carbon Society
  • Research Area : Natural Science > Natural Science General > Other Natural Sciences General
  • Received : March 10, 2023
  • Accepted : May 23, 2023
  • Published : October 1, 2023

Bekmezci Muhammed 1 Bayat Ramazan 1 Akin Merve 1 Coguplugil Zeynep Kazel 1 Sen Fatih 1

1SRG Incorporated Company, Kutahya Design Technopole, Calca OSB Neighbourhood, 431000, Kütahya, Turkey

Accredited

ABSTRACT

MicroRNAs (miRNAs) are emerging materials as ideal biomarkers for noninvasive cancer detection in the early phase. In this article, a simple and label-free electrochemical miRNA biosensor was developed. A single-stranded DNA (ss-DNA) probes were successfully mapped to f-MWCNT and hybridized with the target miR-141 sequence. The optimum peak points of the obtained hybridization were determined using Cyclic Voltammetry (CV) and Differential Pulse Voltammetry (DPV) methods. Significant peaks were observed in the results, depending on miR-141 at different concentrations. The linear relationship (ν) between redox peak currents (Ip) and scanning rate indicated that electron transfer (ET) between miR-141 and the electrode surface was accomplished successfully. In DPV measurements, miR-141 was measured with a low detection limit (LOD) in the 1.3–12 nM concentration range, and the LOD and limit of quantification (LOQ) results were found to be 3 and 9.1 pM, respectively. Besides, selectivity test was investigated for the biosensor using different target analytes and a significant difference in value was observed between the peak currents of miR-141, and other target molecules. This developed strategy has been found to detect miR-141 sensitively, selectively and without tags, and its integration into mobile devices has been successfully carried out.

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