@article{ART003273376},
author={Najmeh Zare and Hassan Karimi‑Maleh and Zhouxiang Zhang and Li Fu and Jalal Rouhi and Nianbing Zhong and Yangping Wen and Masoumeh Ghalkhani},
title={Enhancing cancer biomarker identification: precise monitoring of MUC1 using V2C/Au nanocomposite-amplified electrochemical biosensor},
journal={Carbon Letters},
issn={1976-4251},
year={2025},
volume={35},
number={4},
pages={1691-1700},
doi={10.1007/s42823-025-00887-6}
TY - JOUR
AU - Najmeh Zare
AU - Hassan Karimi‑Maleh
AU - Zhouxiang Zhang
AU - Li Fu
AU - Jalal Rouhi
AU - Nianbing Zhong
AU - Yangping Wen
AU - Masoumeh Ghalkhani
TI - Enhancing cancer biomarker identification: precise monitoring of MUC1 using V2C/Au nanocomposite-amplified electrochemical biosensor
JO - Carbon Letters
PY - 2025
VL - 35
IS - 4
PB - Korean Carbon Society
SP - 1691
EP - 1700
SN - 1976-4251
AB - This work concentrates on the design and implementation of aptamer-based electrochemical biosensors using a layer-by-layer approach for precise tracking of mucin-1 (MUC1), an important biomarker linked to breast cancer. The electrochemical biosensor was created by modifying a screen-printed carbon electrode (SPCE) with V2C MXene booster and gold nanoparticles (Au-NPs), along with Cd2+ integrated aptamer (AP) (SPCE/V2C-MXene/Au NPs/Cd2+-AP). This biosensor demonstrated high specificity and affinity for MUC1, establishing a sensitive quantification mechanism. The MXene nanolayer was produced and analyzed via TEM, XPS, SEM, AFM, BET, and MAP techniques. It served as a supportive material that enhanced electrochemical conductivity and allowed for the integration of the aptamer (AP) as the biological recognition component. The biosensor was constructed by immobilizing MUC1-specific aptamers onto the surfaces of SPCE/V2C-MXene/Au NPs, enabling selective recognition and binding with MUC1. The recorded signal, corresponding to Cd2+ integrated with AP at SPCE/V2C-MXene/Au NPs/Cd2+-AP, enabled quantitative assessment of MUC1 levels. The findings showed a linear concentration span of 1.0–500 pg/mL for detecting MUC1, achieving a detection limit of 3.45 fg/mL utilizing the SPCE/V2C-MXene/Au NPs/Cd2+-AP biosensor. The SPCE/V2C-MXene/Au NPs/Cd2+-AP biosensor exhibited a good affinity for the detection of MUC1 in the presence of other breast cancer biomarkers, confirming its selectivity.
KW - MUC1;V2C MXene;Hybrid composite;Biosensor;Aptamer;Electrochemica
DO - 10.1007/s42823-025-00887-6
ER -
Najmeh Zare, Hassan Karimi‑Maleh, Zhouxiang Zhang, Li Fu, Jalal Rouhi, Nianbing Zhong, Yangping Wen and Masoumeh Ghalkhani. (2025). Enhancing cancer biomarker identification: precise monitoring of MUC1 using V2C/Au nanocomposite-amplified electrochemical biosensor. Carbon Letters, 35(4), 1691-1700.
Najmeh Zare, Hassan Karimi‑Maleh, Zhouxiang Zhang, Li Fu, Jalal Rouhi, Nianbing Zhong, Yangping Wen and Masoumeh Ghalkhani. 2025, "Enhancing cancer biomarker identification: precise monitoring of MUC1 using V2C/Au nanocomposite-amplified electrochemical biosensor", Carbon Letters, vol.35, no.4 pp.1691-1700. Available from: doi:10.1007/s42823-025-00887-6
Najmeh Zare, Hassan Karimi‑Maleh, Zhouxiang Zhang, Li Fu, Jalal Rouhi, Nianbing Zhong, Yangping Wen, Masoumeh Ghalkhani "Enhancing cancer biomarker identification: precise monitoring of MUC1 using V2C/Au nanocomposite-amplified electrochemical biosensor" Carbon Letters 35.4 pp.1691-1700 (2025) : 1691.
Najmeh Zare, Hassan Karimi‑Maleh, Zhouxiang Zhang, Li Fu, Jalal Rouhi, Nianbing Zhong, Yangping Wen, Masoumeh Ghalkhani. Enhancing cancer biomarker identification: precise monitoring of MUC1 using V2C/Au nanocomposite-amplified electrochemical biosensor. 2025; 35(4), 1691-1700. Available from: doi:10.1007/s42823-025-00887-6
Najmeh Zare, Hassan Karimi‑Maleh, Zhouxiang Zhang, Li Fu, Jalal Rouhi, Nianbing Zhong, Yangping Wen and Masoumeh Ghalkhani. "Enhancing cancer biomarker identification: precise monitoring of MUC1 using V2C/Au nanocomposite-amplified electrochemical biosensor" Carbon Letters 35, no.4 (2025) : 1691-1700.doi: 10.1007/s42823-025-00887-6
Najmeh Zare; Hassan Karimi‑Maleh; Zhouxiang Zhang; Li Fu; Jalal Rouhi; Nianbing Zhong; Yangping Wen; Masoumeh Ghalkhani. Enhancing cancer biomarker identification: precise monitoring of MUC1 using V2C/Au nanocomposite-amplified electrochemical biosensor. Carbon Letters, 35(4), 1691-1700. doi: 10.1007/s42823-025-00887-6
Najmeh Zare; Hassan Karimi‑Maleh; Zhouxiang Zhang; Li Fu; Jalal Rouhi; Nianbing Zhong; Yangping Wen; Masoumeh Ghalkhani. Enhancing cancer biomarker identification: precise monitoring of MUC1 using V2C/Au nanocomposite-amplified electrochemical biosensor. Carbon Letters. 2025; 35(4) 1691-1700. doi: 10.1007/s42823-025-00887-6
Najmeh Zare, Hassan Karimi‑Maleh, Zhouxiang Zhang, Li Fu, Jalal Rouhi, Nianbing Zhong, Yangping Wen, Masoumeh Ghalkhani. Enhancing cancer biomarker identification: precise monitoring of MUC1 using V2C/Au nanocomposite-amplified electrochemical biosensor. 2025; 35(4), 1691-1700. Available from: doi:10.1007/s42823-025-00887-6
Najmeh Zare, Hassan Karimi‑Maleh, Zhouxiang Zhang, Li Fu, Jalal Rouhi, Nianbing Zhong, Yangping Wen and Masoumeh Ghalkhani. "Enhancing cancer biomarker identification: precise monitoring of MUC1 using V2C/Au nanocomposite-amplified electrochemical biosensor" Carbon Letters 35, no.4 (2025) : 1691-1700.doi: 10.1007/s42823-025-00887-6
