The Tridosha theory, which is the cornerstone of Ayurvedic physiology governs all the functions of human body and mind. Tridosha are responsible in determining one’s Prakriti and their functional status may vary in both gender of different Prakriti. No research work is available to assess the functions of Dosha by objective parameters. Therefore, this study was planned to find out the variation in functional status of different types of Pitta, using certain objective parameters, in 201 young healthy volunteers of both gender belonging to different Prakriti. Serum level of triglycerides, cholesterol, total protein and glucose level were estimated for Pachaka Pitta and hemoglobin concentration for Ranjaka Pitta, visual acuity for Alochaka Pitta, memory and reaction time for Sadhaka Pitta and RGB value for Bhrajaka Pitta were measured .Except the functioning of Bhrajaka Pitta, variation in functional status of all type of Pitta was not the same in different Prakriti of both gender. However these findings were not significant which may have occurred due to small sample size and homogenous population. Thus we propose the consideration of sex differences while planning and evaluating the studies that are based on Prakriti.