Characterization of Bacillus anthracis proteases through protein-protein interaction: an in silico study of anthrax pathogenicity

Amrita Banerjee; Pradeep Kumar Das Mohapatra; Shilpee Pal; Keshab Chandra Mondal; Bikash Ranjan Pati; Arnab Sen; Tanmay Paul

doi:10.5667/tang.2013.0031

Characterization of Bacillus anthracis proteases through protein-protein interaction: an in silico study of anthrax pathogenicity

CELLMED
Abbr : CellMed
2014, 4(1), pp.6-6
DOI : 10.5667/tang.2013.0031
Publisher : Cellmed Orthocellular Medicine and Pharmaceutical Association
Research Area : Medicine and Pharmacy > General Medicine

Amrita Banerjee ¹, Pradeep Kumar Das Mohapatra ¹, Shilpee Pal ¹, Keshab Chandra Mondal ¹, Bikash Ranjan Pati ¹, Arnab Sen ², Tanmay Paul ¹

¹Vidyasagar University
²University of North Bengal

ABSTRACT

Anthrax is the deadly disease for human being caused by Bacillus anthracis. Instantaneous research work on the mode of infection of the organism revealed that different proteases are involved in different steps of pathogenesis. Present study reports the in silico characterization and the detection of pathogenic proteases involved in anthrax infection through protein-protein interaction. A total of 13 acid, 9 neutral, and 1 alkaline protease of Bacillus anthracis were selected for analysing the physicochemical parameter, the protein superfamily and family search, multiple sequence alignment, phylogenetic tree construction, protein-protein interactions and motif finding. Among the 13 acid proteases, 10 were found as extracellular enzymes that interact with immune inhibitor A (InhA) and help the organism to cross the blood brain barrier during the process of infection. Multiple sequence alignment of above acid proteases revealed the position 368, 489, and 498-contained 100% conserved amino acids which could be used to deactivate the protease. Among the groups analyzed, only acid protease were found to interact with InhA, which indicated that metalloproteases of acid protease group have the capability to develop pathogenesis during B. anthracis infection. Deactivation of conserved amino acid position of germination protease can stop the sporulation and germination of B anthracis cell. The detailed interaction study of neutral and alkaline proteases could also be helpful to design the interaction network for the better understanding of anthrax disease.

KEYWORDS

Anthrax, Bacillus anthracis, protease, superfamily and family, phylogenetic tree, motif, protein-protein interaction

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[journal] Baillie L / 2001 / The development of new vaccines against Bacillus anthracis / J Appl Microbiol 91 : 609~613

[journal] Banerjee A / 2012 / Characterization of tannase protein sequences of bacteria and fungi : an in silico study / Protein J 31 : 306~327

[thesis] Chertow JH / 2011 / Bacillus anthracis protease regulates bacterial adhesion / George Mason University

[journal] Dubey AK / 2010 / In silico characterization of pectate lyase protein sequences from different source organisms / Enzyme Res 2010 : 1~11

[journal] Guruprasad K / 1990 / Correlation between stability of a protein and its dipeptide composition : a novel approach for predicting in vivo stability of a protein from its primary sequence / Protein Eng 4 : 155~161

[journal] Huson DH / 2007 / Dendroscope : An interactive viewer for large phylogenetic trees / BMC Bioinform 8 : 460~464

[journal] John WE / 2010 / Serum protease cleavage of Bacillus anthracis protective antigen / J Biol Chem 285 : 8130~8137

[journal] Kyte J / 1982 / A simple method for displaying the hydropathic character of a protein / J Mol Biol 157 : 105~132

[journal] Larkin MA / 2007 / Clustal W and clustal X version 2.0 / Bioinformatics 23 : 2947~2948

[journal] Morya VK / 2012 / In silico characterization of alkaline proteases from different species of Aspergillus / Appl Biochem Biotechnol 166 : 243~257

[journal] Mukherjee DV / 2011 / Bacillus anthracis protease inha increases blood-brain barrier permeability and contributes to cerebral hemorrhages / PLoS ONE 6 : 1~11

[thesis] Pflughoeft KJ / 2010 / The Immune Inhibitor A1 Protease of Bacillus anthracis / School of Biomedical Sciences in the University of Texas

[journal] Pflughoeft KJ / 2014 / Modulation of the Bacillus anthracis secretome by the immune inhibitor a1 protease / J Bacteriol 196 : 424~435

[journal] Pomerantsev AP / 2011 / A Bacillus anthracis strain deleted for six proteases serves as an effective host for production of recombinant proteins / Protein Expr Purif 80 : 80~90

[journal] Rogers S / 1986 / Amino acid sequences common to rapidly degraded proteins : the PEST hypothesis / Science 234 : 364~368

[journal] Russell BH / 2007 / Bacillus anthracis internalization by human fibroblasts and epithelial cells / Cell Microbiol 9 : 1262~1274

[book] Sheppard MR / 2007 / Robbins Basic Pathology / Saunders : 1~122

[journal] Shrivastava S / 2007 / In silico studies for evaluating conservation homology among family 11 xylanases from Thermomyces lanuginosus / JASES 2 : 70~76

[journal] Tonry JH / 2012 / Bacillus anthracis protease InhA regulates BslA-mediated adhesion in human endothelial cells / Cell Microbiol 14 : 1219~1230

[journal] Tuimala J / 1989 / A primer to phylogenetic analysis using the PHYLIP package / Cladistics 5 : 164~166

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Characterization of Bacillus anthracis proteases through protein-protein interaction: an in silico study of anthrax pathogenicity

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Characterization of Bacillus anthracis proteases through protein-protein interaction: an in silico study of anthrax pathogenicity

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