CELLMED 2023 KCI Impact Factor : 0.03
-
pISSN : 3022-6805 / eISSN : 3022-6791
- https://journal.kci.go.kr/cellmed
KJCKorea
Journal Central
Screening and isolation of antibacterial proteinaceous compounds from flower tissues: Alternatives for treatment of healthcare-associated infections
- CELLMED
- Abbr : CellMed
- 2014, 4(1), pp.5-5
- DOI : 10.5667/tang.2013.0026
- Publisher : Cellmed Orthocellular Medicine and Pharmaceutical Association
- Research Area : Medicine and Pharmacy > General Medicine
Renato Goulart de Almeida 1, Simoni Campos Dias 1, Osmar Nascimento Silva 1, Elizabete de Souza Cândido 1, João Suender Moreira 1, Dianny Elizabeth Jimenez Jojoa 1, Diego Garcês Gomes 1, Mirna de Souza Freire 2, Pedro Henrique de Miranda Bürgel 1, Nelson Gomes de Oliveira Júnior 1, Jorge William Arboleda Valencia 3, Octávio Luiz Franco 2
1Universidade Catolica
2Programa de Pós-Graduação em Ciências Ge
3Universidad del Atlántico
ABSTRACT
Healthcare-associated infection represents a frequent cause of mortality that increases hospital costs. Due to increasing microbial resistance to antibiotics, it is necessary to search for alternative therapies. Consequently, novel alternatives for the control of resistant microorganisms have been studied. Among them, plant antimicrobial protein presents enormous potential, with flowers being a new source of antimicrobial molecules. In this work, the antimicrobial activity of protein-rich fractions from flower tissues from 18 different species was evaluated against several human pathogenic bacteria. The results showed that protein-rich fractions of 12 species were able to control bacterial development. Due its broad inhibition spectrum and high antibacterial activity, the protein-rich fraction of Hibiscus rosa-sinensis was subjected to DEAE-Sepharose chromatography, yielding a retained fraction and a non-retained fraction. The retained fraction inhibits 29.5% of Klebsiella pneumoniae growth, and the non-retained fraction showed 31.5% of growth inhibition against the same bacteria. The protein profile of the chromatography fractions was analyzed by using SDS-PAGE, revealing the presence of two major protein bands in the retained fraction, of 20 and 15 kDa. The results indicate that medicinal plants have the biotechnological potential to increase knowledge about antimicrobial protein structure and action mechanisms, assisting in the rational design of antimicrobial compounds for the development of new antibiotic drugs.
Statistics
-
362 Viewed
-
58 Downloaded
Tools
Issue List
Citation status
* References for papers published after 2023 are currently being built.
[journal]
Antoniw JF
/ 1980
/ Comparison of three pathogenesis-related proteins from plants of two cultivars of tobacco infected with TMV
/ J Gen Virol
47
: 79~87
[journal]
Apostolopoulou E
/ 2004
/ Nosocomial bloodstream infections in a neonatal intensive care unit
/ Br J Nurs
13
: 806~812
[journal]
Arullappan S
/ 2009
/ Preliminary screening of antibacterial activity using crude extracts of Hibiscus rosa sinensis
/ Trop Life Sci Res
20
: 109~118
[journal]
Benko-Iseppon AM
/ 2010
/ Ethnobotanical bioprospection of candidates for potential antimicrobial drugs from Brazilian plants : state of art and perspectives
/ Curr Protein Pept Sci
11
: 189~194
[journal]
Berrocal-Lobo M
/ 2002
/ A snakin-2, an antimicrobial peptide from potato whose gene is locally induced by wounding and responds to pathogen infection
/ Plant Physiol
128
: 951~961
[journal]
Blum H
/ 1987
/ Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels
/ Electrophoresis
8
: 93~99
[journal]
Candido ES
/ 2011
/ Plant storage proteins with antimicrobial activity : novel insights into plant defense mechanisms
/ FASEB J
25
: 3290~3305
[journal]
Capella AN
/ 2001
/ COI1 affects myrosinase activity and controls the expression of two flowerspecific myrosinase-binding protein homologues in Arabidopsis
/ Planta
213
: 691~699
[journal]
Castro MS
/ 2005
/ Plant defense and antimicrobial peptides
/ Protein Pept Lett
12
: 13~18
[book]
Clinical and Laboratory Institute
/ 2009
/ Methods for Diluition Antimicrobial Susceptibility Tests Bacteria That Grow Aerobically
/ CLSI document
: M07~A8
[journal]
de Beer A
/ 2011
/ Four plant defensins from an indigenous South African Brassicaceae species display divergent activities against two test pathogens despite high sequence similarity in the encoding genes
/ BMC Res Notes
4
: 459~
[journal]
Endringer DC
/ 2010
/ Evaluation of Brazilian plants on cancer chemoprevention targets in vitro
/ Phytother Res
24
: 928~933
[journal]
Favarin DC
/ 2013
/ Potential effects of medicinal plants and secondary metabolites on acute lung injury
/ Biomed Res Int
2013
: 1~12
[journal]
Ferreira PM
/ 2011
/ Study of the antiproliferative potential of seed extracts from Northeastern Brazilian plants
/ An Acad Bras Cienc
83
: 1045~1058
[journal]
Ferreira RB
/ 2007
/ The role of plant defense proteins in fungal pathogenesis
/ Mol Plant Pathol
8
: 677~700
[journal]
Garcia-Olmedo F
/ 2001
/ Antibiotic activities of peptides, hydrogen peroxide and peroxynitrite in plant defense
/ FEBS Lett
498
: 219~222
[journal]
Guani-Guerra E
/ 2010
/ Antimicrobial peptides : general overview and clinical implications in human health and disease
/ Clin Immunol
135
: 1~11
[journal]
Guimaraes G
/ 2012
/ Cytogenetic characterization and genome size of the medicinal plant Catharanthus roseus (L.) G
/ Don. AoB Plants
2012
: pls002~
[book]
Hughes RG
/ 2008
/ AHRQ Publication
/ Agency for Healthcare Research and Quality
: 1403~
[journal]
Iriti M
/ 2007
/ Review of innate and specific immunity in plants and animals
/ Mycopathologia
164
: 57~64
[journal]
Koo JC
/ 1998
/ Two hevein homologs isolated from the seed of Pharbitis nil L. exhibit potent antifungal activity
/ Biochim Biophys Acta
1382
: 80~90
[journal]
Kovalskaya N
/ 2009
/ Expression and functional characterization of the plant antimicrobial snakin-1 and defensin recombinant proteins
/ Protein Expr Purif
63
: 12~17
[journal]
Laemmli UK
/ 1970
/ Cleavage of structural proteins during the assembly of the head of bacteriophage T4
/ Nature
227
: 680~685
[journal]
Lay FT
/ 2003
/ Isolation and properties of floral defensins from ornamental tobacco and petunia
/ Plant Physiol
131
: 1283~1293
[journal]
Lay FT
/ 2003
/ The three-dimensional solution structure of NaD1, a new floral defensin from Nicotiana alata and its application to a homology model of the crop defense protein alfAFP
/ J Mol Biol
325
: 175~188
[journal]
Leitao SG
/ 2006
/ Screening of Central and South American plant extracts for antimycobacterial activity by the Alamar Blue test
/ Braz J Pharmacogn
16
: 6~11
[journal]
Lima TB
/ 2011
/ A Kunitz proteinase inhibitor from corms of Xanthosoma blandum with bactericidal activity
/ J Nat Prod
74
: 969~975
[journal]
Lin KF
/ 2005
/ Characterization and structural analyses of nonspecific lipid transfer protein 1 from mung bean
/ Biochemistry
44
: 5703~5712
[journal]
Liu J-J
/ 2006
/ The family 10 of plant pathogenesis-related proteins: Their structure, regulation, and function in response to biotic and abiotic stresses
/ Physiol Mol Plant Pathol
68
: 3~13
[journal]
Lotan T
/ 1989
/ Pathogenesis-related proteins are developmentally regulated in tobacco flowers
/ Plant Cell
1
: 881~887
[journal]
Mandal SM
/ 2012
/ Identification and characterization of a bactericidal and proapoptotic peptide from Cycas revoluta seeds with DNA binding properties
/ J Cell Biochem
113
: 184~193
[journal]
Mandal SM
/ 2011
/ Identification of an antifungal peptide from Trapa natans fruits with inhibitory effects on Candida tropicalis biofilm formation
/ Peptides
32
: 1741~1747
[journal]
Maria-Neto S
/ 2011
/ Bactericidal activity identified in 2S Albumin from sesame seeds and in silico studies of structure-function relations
/ Protein J
30
: 340~350
[journal]
Marinho DG
/ 2011
/ The latex obtained from Hancornia speciosa Gomes possesses anti-inflammatory activity
/ J Ethnopharmacol
135
: 530~537
[journal]
Matsuhira H
/ 2007
/ An anther-specific lipid transfer protein gene in sugar beet : its expression is strongly reduced in malesterile plants with owen cytoplasm
/ Physiol Plant
129
: 407~414
[journal]
Mohammed MJ
/ 2009
/ Isolation and identification of antibacterial compounds from Thymus kotschyanus aerial parts and Dianthus caryophyllus flower buds
/ Phytomedicine
16
: 632~637
[journal]
Morais SM
/ 2005
/ Ethnomedicinal plants of tapeba indians from the state of Ceara-Brazil
/ Braz J Pharmacogn
15
: 169~177
[journal]
Moreira JS
/ 2011
/ Identification of botryticidal proteins with similarity to NBS-LRR proteins in rosemary pepper (Lippia sidoides Cham.) flowers
/ Protein J
30
: 32~38
[journal]
Pelegrini PB
/ 2011
/ Antibacterial peptides from plants : what they are and how they probably work
/ Biochem Res Int
2011
: 250349~
[journal]
Pelegrini PB
/ 2008
/ Identification of a novel storage glycine-rich peptide from guava(Psidium guajava)seeds with activity against Gramnegative bacteria
/ Peptides
29
: 1271~1279
[journal]
Petrosillo N
/ 2010
/ Management of antibiotic resistance in the intensive care unit setting
/ Expert Rev Anti Infect Ther
8
: 289~302
[journal]
Pereira AB
/ 2012
/ Development and validation of an HPLC-DAD method for quantification of bornesitol in extracts from Hancornia speciosa leaves after derivatization with ptoluenesulfonyl chloride
/ J Chromatogr B Analyt Technol Biomed Life Sci
887-888
: 133~137
[journal]
Pittet D
/ 2008
/ Infection control as a major World Health Organization priority for developing countries
/ J Hosp Infect
68
: 285~292
[journal]
Rahmatullah M
/ 2012
/ Medicinal plants used for treatment of diabetes by the Marakh sect of the Garo tribe living in Mymensingh district, Bangladesh
/ Afr J Tradit Complement Altern Med(AJTCAM)
9
: 380~385
[journal]
Rask L
/ 2000
/ Myrosinase : gene family evolution and herbivore defense in Brassicaceae
/ Plant Mol Biol
42
: 93~113
[journal]
Ribeiro SM
/ 2011
/ Identification of a Passiflora alata Curtis dimeric peptide showing identity with 2S albumins
/ Peptides
32
: 868~874
[journal]
Rodrigues Orsi P
/ 2012
/ Hymenaea stigonocarpa Mart. ex Hayne: a Brazilian medicinal plant with gastric and duodenal anti-ulcer and antidiarrheal effects in experimental rodent models
/ J Ethnopharmacol
143
: 81~90
[journal]
Ruban P
/ 2012
/ In vitro antibacterial activity of Hibiscus rosa-sinensis flower extract against human pathogens
/ Asian Pac J Trop Biomed
2
: 399~403
[journal]
Ruef C
/ 2005
/ Nosocomial infections in intensive care units
/ Infection
33
: 105~
[journal]
Sartori MR
/ 2003
/ Antifungal activity of fractions and two pure compounds of flowers from Wedelia paludosa(Acmela brasiliensis)(arastaceae)
/ Pharmazie
58
: 567~569
[journal]
Samy RP
/ 2013
/ Evaluation of Aromatic Plants and Compounds Used to Fight Multidrug Resistant Infections
/ Evid Based Complement Alternat Med
2013
: 1~17
[journal]
Segura A
/ 1999
/ Snakin-1, a peptide from potato that is active against plant pathogens
/ Mol Plant Microbe Interact
12
: 16~23
[journal]
Sels J
/ 2008
/ Plant pathogenesis-related(PR)proteins : a focus on PR peptides
/ Plant Physiol and Biochem
46
: 941~950
[journal]
Shabir G
/ 2011
/ Antioxidant and antimicrobial attributes and phenolics of different solvent extracts from leaves, flowers and bark of Gold Mohar [Delonix regia (Bojer ex Hook.) Raf]
/ Molecules
16
: 7302~7319
[journal]
Shakir T
/ 2013
/ An exploration of the potential mechanisms and translational potential of five medicinal plants for applications in Alzheimer's disease
/ Am J Neurodegener Dis
2
: 70~88
[journal]
Silva AR
/ 2012
/ Chemical composition, antioxidant and antibacterial activities of two Spondias species from Northeastern Brazil
/ Pharm Biol
50
: 740~746
[journal]
Silva GC
/ 2011
/ Hancornia speciosa Gomes induces hypotensive effect through inhibition of ACE and increase on NO
/ J Ethnopharmacol
137
: 709~713
[journal]
Silva ON
/ 2011
/ Exploring the pharmacological potential of promiscuous hostdefense peptides : from natural screenings to biotechnological applications
/ Front Microbiol
2
: 232~
[journal]
Sousa Araujo TA
/ 2012
/ Habitat influence on antioxidant activity and tannin concentrations of Spondias tuberosa
/ Pharm Biol
50
: 754~759
[journal]
Tavares LS
/ 2008
/ Biotechnological potential of antimicrobial peptides from flowers
/ Peptides
29
: 1842~1851
[journal]
Thevissen K
/ 1999
/ Permeabilization of fungal membranes by plant defensins inhibits fungal growth
/ Appl Environ Microbiol
65
: 5451~5458
[journal]
Van Damme EJ
/ 1999
/ A gene encoding a hevein-like protein from elderberry fruits is homologous to PR-4 and class V chitinase genes
/ Plant Physiol
119
: 1547~1556
[journal]
Van der Weerden NL
/ 2008
/ The plant defensin, NaD1, enters the cytoplasm of Fusarium Oxysporum Hyphae
/ J Biol Chem
283
: 14445~14452
[journal]
Vasudeva N
/ 2008
/ Post-Coital Antifertility Activity of Hibiscus rosa-sinensis Linn. roots
/ Evid Based Complement Alternat Med
5
: 91~94
[journal]
Wong CT
/ 2012
/ Optimal oxidative folding of the novel antimicrobial cyclotide from Hedyotis biflora requires high alcohol concentrations
/ Biochemistry
50
: 7275~7283