Alkaloids of Linderae Radix suppressed the lipopolysaccharide-induced expression of cytokines in cultured macrophage RAW 264.7 cells

David Jiyao Chou; Kelly Yinching Lam; Jianping Chen; Ping Yao; Tina Tingxia Dong; Aizhen Xiong; Guixin Chou; Zhengtao Wang; Karl Wah-Keung Tsim

Alkaloids of Linderae Radix suppressed the lipopolysaccharide-induced expression of cytokines in cultured macrophage RAW 264.7 cells

CELLMED
Abbr : CellMed
2014, 4(4), pp.28-28
Publisher : Cellmed Orthocellular Medicine and Pharmaceutical Association
Research Area : Medicine and Pharmacy > General Medicine

David Jiyao Chou ¹, Kelly Yinching Lam ¹, Jianping Chen ¹, Ping Yao ¹, Tina Tingxia Dong ¹, Aizhen Xiong ², Guixin Chou ², Zhengtao Wang ², Karl Wah-Keung Tsim ¹

¹Hong Kong University
²Shanghai University

ABSTRACT

Linderae Radix, the dry roots of Lindera aggregata (Sims) Kosterm, has long been used as traditional Chinese medicine for treatment of inflammatory diseases. The total alkaloids are believed to be the active components responsible for anti-inflammation of Linderae Radix. Here, the total alkaloids of Linderae Radix were extracted and isolated, including 12 isoquinoline alkaloids and 1 furan sesquiterpene. Within the alkaloids, norisoboldine, boldine, linderaline, isoboldine, reticuline, N-methyllaurotetanine, norjuziphine were found to be the major ingredients. In lipopolysaccharide-treated macrophage RAW 264.7 cells, application of Linderae Radix extract, or total alkaloids, suppressed the transcription of pro-inflammatory cytokines, interleukin-1β and interleukin-6. Out of the 12 alkaloids, norisoboldine, boldine, and isoboldine were tested in lipopolysaccharide-treated macrophages, and norisoboldine was the strongest alkaloid in suppressing the cytokine expressions. The current studies suggested that the identification of alkaloids from Linderae Radix could provide a plausible explanation for herbal therapeutic functions.

KEYWORDS

traditional Chinese medicine, Linderae Radix, total alkaloids, isoquinoline alkaloids, pro-inflammatory cytokines

Citation status

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