CELLMED 2023 KCI Impact Factor : 0.03
-
pISSN : 3022-6805 / eISSN : 3022-6791
- https://journal.kci.go.kr/cellmed
KJCKorea
Journal Central
Effects of the Fraction of SambucusWilliamsii, NNMBS 246, on Osteoblastic Differentiation
- CELLMED
- Abbr : CellMed
- 2018, 8(3), pp.13-13
- Publisher : Cellmed Orthocellular Medicine and Pharmaceutical Association
- Research Area : Medicine and Pharmacy > General Medicine
Soon-Il Kang 1, Jaesuh Park 2, Kwon Il Keun 2, Eun-Cheol Kim 1
1Kyung Hee University
2경희대학교
Candidate
ABSTRACT
In the field of osteoporosis, there has been growing interest in anabolic agents that enhance bone formation. The purpose of this study was to examine the effects of NNMBS 246 ostoblastic differentiation with associated signaling pathways. NNMBS 246 markedly increased alkaline phosphatase (ALP) activity and calcium nodule formation. Stimulation with NNMBS 246 not only increased the differentiation markers (ALP, OPN, OCN) level and transcription markers (RUNX2, Osterix) mRNA expression but also upregulated the ECM molecules and OPG mRNA expression. Treatments of NNMBS 246 downregulated MMPs (MMP-1, MMP-2, MMP-9), but RANKL mRNA expression. Furthermore, NNMBS 246 activated osteoblastic differentiation markers and formed calcium nodules in human periodontal ligament cells (hPDLCs) and cementoblast cells. NNMBS 246 induced phosphorylation of MAPKs, Akt, nuclear p65 and IkB-α. BMP-2/Smad and β-catenin signaling pathways were activated by NNMBS 246. Sirtinol (SIRT1 inhibitor) inhibited NNMBS 246-induced osteoblastic differentiation markers mRNA expression. These results suggested that NNMBS 246 has the potential to enhance osteoblastogenesis probably through the activation of BMP/Smad and β-catenin signal pathways, and SIRT1 plays as critical mediator in bone anabolic effect of NNMBS 246.
Statistics
-
369 Viewed
-
46 Downloaded
Tools
Issue List
Citation status
* References for papers published after 2023 are currently being built.
[journal]
Backesjo CM
/ 2006
/ Activation of Sirt1 decrease adipocyte formation during osteoblast differentiation of mesenchymal stem cells
/ J Bone Miner Res
21
: 993~1002
[journal]
Bai L
/ 2008
/ Modulation of Sirt1 by resveratrol and nicotinamide alters proliferation and differentiation of pig preadipocytes
/ Mol Cell Biochem
307
: 129~140
[journal]
Bartold PM
/ 2000
/ Tissue engineering: a new paradigm for periodontal regeneration based on molecular and cell biology
/ Periodontol 2000
(24)
: 253~269
[journal]
Blander G
/ 2009
/ SIRT1 promotes differentiation of normal human keratinocytes
/ J Invest Dermatol
129
: 41~49
[journal]
Bolander ME
/ 1992
/ Regulation of fracture repair by growth factors
/ Proc Soc Exp Biol Med
200
: 165~170
[journal]
Dai Z
/ 2007
/ Resveratrol enhances proliferation and osteoblastic differentiation in human mesenchymal stem cells via ER-dependent ERK1/2 activation
/ Phytomedicine
14
: 806~814
[journal]
De Biase P
/ 2005
/ Clinical applications of BMPs
/ Injury
36(Suppl 3)
: S43~S46
[journal]
Eliseev RA
/ 2006
/ Smad7 mediates inhibition of Saos2 osteosarcoma cell differentiation by NF-kB
/ Exp Cell Res
312
: 40~50
[journal]
Dryden SC
/ 2003
/ Role for human SIRT2 NAD-dependent deacetylase activity in control of mitotic exit in the cell cycle, Mol
/ Cell Biol
23
: 3173~3185
[journal]
Fujita T
/ 2004
/ Runx2 induces osteoblast and chondrocyte differentiation and enhances their migration by coupling with PI3-Akt signaling
/ J Cell Biol
166
: 85~95
[journal]
Fulco M
/ 2003
/ Sir2 regulates skeletal muscle differentiation as a potential sensor of the redox state
/ Mol Cell
12
: 51~62
[journal]
Hassumi MY
/ 2009
/ PPAR-gamma agonist rosiglitazone prevents inflammatory periodontal bone loss by inhibiting osteoclastogenesis
/ Int Immunopharmacol
9
: 1150~1158
[journal]
Hisahara S
/ 2008
/ Histone deacetylase SIRT1 modulates neuronal differentiation by its nuclear translocation
/ Proc Natl Acad Sci U S A.
105
: 15599~15604
[journal]
Inanc B
/ 2006
/ Osteogenic induction of human periodontal ligament fibroblasts under two- and threedimensional culture conditions
/ Tissue Eng
12
: 257~266
[journal]
Jeong GS
/ 2009
/ Antiinflammatory effects of apigenin on nicotine- and lipopolysaccharide-stimulated human periodontal ligament cells via heme oxygenase-1
/ IntImmunopharmacol
9
: 1374~1380
[journal]
Jeong GS
/ 2010
/ Effects of sappanchalcone on the cytoprotection and anti-inflammation via heme oxygenase-1 in human pulp and periodontal ligament cells
/ Eur J Pharmacol
644
: 230~237
[journal]
Karring T
/ 1993
/ Development of the biological concept of guided tissue regenerationeanimal and human studies
/ Periodontol 2000
1
: 26~35
[journal]
Kawai T
/ 2006
/ B and T lymphocytes are the primary sources of RANKL in the bone resorptive lesion of periodontal disease
/ Am J Pathol
169
: 987~998
[journal]
Krishnan V
/ 2006
/ Regulation of bone mass by Wnt signaling
/ J Clin Invest
116
: 1202~1209
[journal]
Kumar A
/ 2010
/ NF-kappaB inhibitory action of resveratrol: a probable mechanism of neuroprotection in experimental diabetic neuropathy
/ BiochemBiophys Res Commun
394
: 360~365
[journal]
Lee FS
/ 1997
/ Activation of the IkappaB alpha kinase complex by MEKK1, a kinase of the JNK pathway
/ Cell
88
: 213~222
[journal]
Lee KS
/ 2000
/ Runx2 is a common target of transforming growth factor beta1 and bone morphogenetic protein 2, and cooperation between Runx2 and Smad5 induces osteoblast-specific gene expression in the pluripotent mesenchymal precursor cell line C2C12
/ Mol Cell Biol
20
: 8783~8792
[book]
Lee TB
/ 1998
/ Coloured Flora of Korea, Vol. 2
/ Hyang-Moon Sa
: 229~
[journal]
Lieberman JR
/ 2002
/ The role of growth factors in the repair of bone.Biology and clinical applications
/ J Bone Joint Surg Am
84-A
: 1032~1044
[journal]
Matsuguchi T
/ 2009
/ JNK Activity Is Essential for Atf4 Expression and Late-Stage Osteoblast Differentiation
/ J Bone Miner Res
24
: 398~410
[journal]
Miyamoto T
/ 2003
/ Differentiation and function of osteoclasts
/ Keio J Med
52
: 1~7
[journal]
Mizutani K
/ 1998
/ Resveratrol stimulates the proliferationand differentiation of osteoblastic MC3T3-E1 cells
/ Biochem Biophys Res
253
: 859~863
[journal]
Mundy G
/ 1999
/ Stimulation of bone formation in vitro and in rodents by statins
/ Science
286
: 1946~1949
[journal]
Nohe A
/ 2004
/ Signal transduction of bone morphogenetic protein receptors
/ Cell Signal
16
: 291~299
[journal]
Ota H
/ 2006
/ Sirt1 inhibitor, Sirtinol, induces senescence-like growth arrest with attenuated Ras-MAPK signaling in human cancer cells
/ Oncogene
25
: 176~185
[journal]
Picard F
/ 2004
/ Sirt1 promotes fat mobilization in white adipocytes by repressing PPAR-gamma
/ Nature
429
: 771~776
[journal]
Prince M
/ 2001
/ Expression and regulation of Runx2/Cbfa1 and osteoblast phenotypic markers during the growth and differentiation of human osteoblasts
/ J Cell Biochem
80
: 424~440
[journal]
Ripamonti U
/ 1994
/ Periodontal regeneration: potential role of bone morphogenetic proteins
/ J Periodont Res
29
: 225~235
[journal]
Silva TA
/ 2007
/ Chemokines in oral inflammatory diseases: apical periodontitis and periodontal disease
/ J Dent Res
86
: 306~319
[journal]
Tissenbaum HA
/ 2001
/ Increased dosage of a sir-2 gene extends lifespan in Caenorhabditiselegans
/ Nature
410
: 227~230
[journal]
Wang Z
/ 2011
/ Two New Iridoid Glycosides from the Root Barks of Sambucus williamsii
/ Hance Molecules
16(5)
: 3869~3874
[journal]
Xiao H
/ 2014
/ Synthesis and photophysical properties of a novel pyridinium salt based on dipicolinate
/ Fitoterapia
94
: 29~
[journal]
Xie F
/ 2005
/ Increase in bone mass and bone strength by Sambucus williamsii HANCE in ovariectomized rats
/ Biol Pharm Bull
28
: 1879~1885
[journal]
Yamamoto N
/ 1997
/ Smad1 and smad5 act downstream of intracellular signalings of BMP-2 that inhibits myogenic differentiation and induces osteoblast differentiation in C2C12 myoblasts
/ Biochem Biophys Res Commun
238
: 574~580
[journal]
Youn J
/ 2009
/ Resveratrol and piceatannol inhibit iNOS expression and NF-kappaB activation in dextran sulfate sodium-induced mouse colitis
/ Nutr Cancer
61
: 847~854
[journal]
Yun JH
/ 2004
/ Inhibitory effects of green tea polyphenol (-)-epigallocatechingallate on the expression of matrix metalloproteinase-9 and on the formation of osteoclasts
/ J Periodontal Res
39
: 300~307
[journal]
Zhao H
/ 2008
/ Effect of SBD.4A--a defined multicomponent preparation of Angelica sinensis--in periodontal regeneration models
/ Phytother Res
22
: 923~928
[journal]
Zhang RH
/ 1997
/
/ Acta Chin. Med. Pharmacol.
4
: 30~
This paper was written with support from the National Research Foundation of Korea.