Providing older adults with routine health screening is an important health care policy in many aging societies including Korea. However, a significant proportion of older adults do not participate in health screening, but the association between their characteristics and their participation in screening is not well known. The present study explored the determinants for the participation of older adults in national health screening using the 1-5 waves of the Korean Longitudinal Study of Ageing (KLoSA) data (n=1,886). Screening participation tends to increase across the study waves, while panel logit models indicated that screening take-up varies by age, marital status, education, household income, living area, type of health insurance, functional disability, and self-rated health. The results suggest increasing efforts of outreach for older adults who are at a greater risk of missing health screening.