Effect of Storage Media and Duration on Pulpal Cell Viability in Exfoliated Deciduous Teeth (탈락 유치 내 치수 세포의 보관 용액과 기간에 따른 생존)

박지원; Je Seon Song (송제선); JAE HO LEE (이제호); SEONG OH KIM (김성오); 전미정; HAN-SUNG JUNG (정한성); Heung Kyu Son (손흥규)

doi:10.5933/JKAPD.2014.41.1.1

Effect of Storage Media and Duration on Pulpal Cell Viability in Exfoliated Deciduous Teeth

Journal of the Korean academy of pediatric dentistry
Abbr : J Korean Acad Pediatr Dent
2014, 41(1), pp.1-7
DOI : 10.5933/JKAPD.2014.41.1.1
Publisher : The Korean Academy Of Pediatric Dentistry
Research Area : Medicine and Pharmacy > Dentistry

박지원 ¹, Je Seon Song ², JAE HO LEE ², SEONG OH KIM ², 전미정 ¹, HAN-SUNG JUNG ², Heung Kyu Son ²

¹연세대학교 치과대학 소아치과학교실
²연세대학교

Accredited

ABSTRACT

If it is possible to preserve and culture cells from exfoliated deciduous teeth in a readily available storagemedium within each family, more stem cells would be obtained. This research is about the effect of storage mediaand time on pulpal cell viability of exfoliated deciduous teeth. 330 exfoliated deciduous teeth were randomly divided into 11 groups; fresh group, dry group, groups stored incell culture medium (2, 4, 7 days each), in milk (2, 4, 7 days each), and in saline (2, 4, 7 days each). Primaryculture of pulpal cells was conducted in each group and the success rates were compared by calculating thenumber of teeth with viable cells. The result of primary culture shows that the success rate decreases as the time of storage gets longer. Therewas no statistical difference between groups stored in the cell culture medium, milk, and saline for 2 and 4days. However, the groups stored in milk and saline for 7 days showed dramatic decrease in success rate comparedto the group stored in the cell culture medium. In conclusion, exfoliated or extracted deciduous teeth can be used to culture pulpal cells when they are storedin milk and saline for a certain period of time; however obtaining viable pulpal cells becomes harder as the storagetime gets longer.

KEYWORDS

Storage medium for deciduous teeth, Pulpal tissue, Cell viability, Milk, Saline

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Journal of the Korean academy of pediatric dentistry 2023 KCI Impact Factor : 0.48

Effect of Storage Media and Duration on Pulpal Cell Viability in Exfoliated Deciduous Teeth

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* References for papers published after 2023 are currently being built.

Journal of the Korean academy of pediatric dentistry 2023 KCI Impact Factor : 0.48

Effect of Storage Media and Duration on Pulpal Cell Viability in Exfoliated Deciduous Teeth

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