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Study of Bacteria Associated with Dental Caries Using a 3 Tone Disclosing Agent

  • Journal of the Korean academy of pediatric dentistry
  • Abbr : J Korean Acad Pediatr Dent
  • 2018, 45(1), pp.32-40
  • DOI : 10.5933/JKAPD.2018.45.1.32
  • Publisher : The Korean Academy Of Pediatric Dentistry
  • Research Area : Medicine and Pharmacy > Dentistry
  • Received : July 7, 2017
  • Accepted : August 31, 2017
  • Published : February 28, 2018

Jeongeun Lee 1 Howon Park 2 LEE JU HYUN 2 Hyunwoo Seo 2 Lee, Si Young 2

1강릉원주대학교 치과대학 소아청소년치과학교실 및 구강과학연구소
2강릉원주대학교

Accredited

ABSTRACT

The aim of this study was to determine the efficacy of a 3 tone plaque disclosing gel in assessing the risk of caries related to the population of Streptococcus mutans , Streptococcus sobrinus , and Lactobacillus spp . quantified using a quantitative real-time polymerase chain reaction (qRT-PCR). 15 healthy children of ages 9 - 12 years were randomly examined. The 3 tone plaque disclosing gel was applied on teeth surfaces, which changed the color to pink or red, blue or purple and light blue. Plaque was divided into 3 groups based on staining. Genomic DNA from each sample was subjected to a qRT-PCR assay for quantitative detection of target bacteria. The Kruskal-Wallis test was conducted for correlation between the color of plaque and the number of bacterial species. The levels of S. mutans , S. sobrinus , and Lactobacillus spp . were significantly different in the plaque samples of the 3 groups (p < 0.05). The proportion of S. sobrinus to S. mutans showed correlation to the color of plaque. The different color-dyed plaque was related to the number of acidogenic bacteria. The 3 tone plaque disclosing gel could be used as one of the indicators to assess the clinical risk of caries associated with the population of S. mutans , S. sobrinus , and Lactobacillus spp .

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