Characteristics of Supernumerary Tooth-derived Pulp Cells during Long-term Culture (과잉치 치수유래 줄기세포의 장기 배양 특성)

Hyunsoo Maeng (맹현수); Kim Jongbin (김종빈); Jong-Soo Kim (김종수)

doi:10.5933/JKAPD.2018.45.4.492

Characteristics of Supernumerary Tooth-derived Pulp Cells during Long-term Culture

Journal of the Korean academy of pediatric dentistry
Abbr : J Korean Acad Pediatr Dent
2018, 45(4), pp.492-498
DOI : 10.5933/JKAPD.2018.45.4.492
Publisher : The Korean Academy Of Pediatric Dentistry
Research Area : Medicine and Pharmacy > Dentistry
Received : June 21, 2018
Accepted : July 5, 2018
Published : November 30, 2018

Hyunsoo Maeng ¹, Kim Jongbin ², Jong-Soo Kim ²

¹단국대학교 치과대학 소아치과학교실
²단국대학교

Accredited

ABSTRACT

This study was conducted to investigate the characteristics of subculture times in the early, middle, and late passages by measuring the time under subculture until it was judged that the supernumerary tooth-derived pulp stem cells (sDPSCS) were no longer proliferating. Three supernumerary teeth from two healthy six-years old boys were extracted and stem cells were obtained from the pulp tissue. This was called SNT1 (supernumerary tooth 1), SNT2, and the supernumerary tooth from another child was named SNT3. SNT1 and 2 were subcultured at the same time and SNT3 was subcultured a little faster. The mean time of complete subculture was 3.6 ± 1.1 days. Total passages were cultured up to 23.3 ± 0.6 and took 83 days. These were divided into three groups based on the passage. The increase rate of time taken in subculture between group I and group II was 11.9%, but the rate between group II and group III was 28.6%, which was 2.4 times increased. The time taken between passages during long-term subculture up to 22 passages shows a regressive pattern y = 0.1169x + 2.25 and y = 0.1169x + 2.0. In conclusion, the passage time of SPSCs increased in late passages, and it shows a similar pattern.

KEYWORDS

Supernumerary tooth, Pulp stem cell, Passage

Citation status

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Journal of the Korean academy of pediatric dentistry 2023 KCI Impact Factor : 0.48

Characteristics of Supernumerary Tooth-derived Pulp Cells during Long-term Culture

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* References for papers published after 2023 are currently being built.

Journal of the Korean academy of pediatric dentistry 2023 KCI Impact Factor : 0.48

Characteristics of Supernumerary Tooth-derived Pulp Cells during Long-term Culture

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