Cytotoxicity of Various Calcium Silicate-based Materials with Stem Cells from Deciduous Teeth

Jihye Yun (윤지혜); Yong-Ouk You (유용욱); Ahn Eunsuk (안은숙); Jun Lee (이준); An So-youn (안소연)

doi:10.5933/JKAPD.2019.46.1.85

Cytotoxicity of Various Calcium Silicate-based Materials with Stem Cells from Deciduous Teeth

Journal of the Korean academy of pediatric dentistry
Abbr : J Korean Acad Pediatr Dent
2019, 46(1), pp.85~92
DOI : 10.5933/JKAPD.2019.46.1.85
Publisher : The Korean Academy Of Pediatric Dentistry
Research Area : Medicine and Pharmacy > Dentistry
Received : July 23, 2018
Accepted : September 6, 2018
Published : February 28, 2019

Jihye Yun ¹, Yong-Ouk You ², Ahn Eunsuk ³, Jun Lee ², An So-youn ²

¹원광대학교 치과대학 소아치과학교실
²원광대학교
³경복대학교

Accredited

ABSTRACT

The purpose of this study was to compare and evaluate the cytotoxicity of 3 calcium silicate-based materials (CSMs) on stem cells from human exfoliated deciduous teeth (SHEDs). The powder of Retro MTA® (RM), EZ-SealTM (EZ) and ENDOCEM Zr® (EN) was eluted with SHED culture media and then filtered. The SHEDs were cultured in the presence of the various concentrations of the eluate. To investigate the effect of the 3 CSMs on SHED proliferation, the MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay was performed. Flow cytometry analysis was also performed to identify any changes in the cellular phenotype. The absorbance values of the SHEDs cultured in the eluate of samples at a 10% concentration showed the following relation: RM > EN > EZ p( = 0.0439). However, the SHEDs maintained their mesenchymal phenotype regardless of product exposure. Although the 3 CSMs did not alter the SHED stem cell markers, EZ may be a less cytocompatible than RM and EN.

KEYWORDS

Cytotoxicity, Calcium silicate, Mineral trioxide aggregate, Deciduous tooth, Stem cells

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[journal] Fuks AB / 2008 / Vital pulp therapy with new materials for primary teeth : new directions and treatment perspectives / J Endod 34 : S18~S24

[journal] Rodd HD / 2006 / Pulp therapy for primary molars / Int J Paediatr Dent 16(Suppl 1) : 15~23

[journal] Gandolfi MG / 2015 / Calcium silicate/calcium phosphate biphasic cements for vital pulp therapy : chemical-physical properties and human pulp cells response / Clin Oral Investig 19 : 2075~2089

[journal] Prati C / 2015 / Calcium silicate bioactive cements : biological perspectives and clinical applications / Dent Mater 31 : 351~370

[journal] Han L / 2015 / Evaluation of calcium-releasing and apatite-forming abilities of fast-setting calcium silicatebased endodontic materials / Int Endod J 48 : 124~130

[journal] Torabinejad M / 2010 / Mineral trioxide aggregate : a comprehensive literature review-part II : leakage and biocompatibility investigations / J Endod 36 : 190~202

[journal] Mente J / 2013 / Treatment outcome of mineral trioxide aggregate in open apex teeth / J Endod 39 : 20~26

[journal] Okiji T / 2009 / Reparative dentinogenesis induced by mineral trioxide aggregate : a review from the biological and physicochemical points of view / Int J Dent 2009 : 464280~

[journal] Kulan P / 2016 / Biocompatibility of accelerated mineral trioxide aggregate on stem cells derived from human dental pulp / J Endod 42 : 276~279

[journal] Holan G / 2005 / Long-term evaluation of pulpotomy in primary molars using mineral trioxide aggregate and formocresol / Pediatr Dent 27 : 129~136

[journal] Stringhini Junior E / 2015 / Evidence of pulpotomy in primary teeth comparing MTA, calcium hydroxide, ferric sulphate, and electrosurgery with formocresol / Eur Arch Paediatr Dent 16 : 303~312

[journal] Chang SW / 2014 / Effects of calcium silicate endodontic cements on biocompatibility and mineralization-inducing potentials in human dental pulp cells / J Endod 40 : 1194~1200

[journal] Lee H / 2015 / Comparative study of pulpal responses to pulpotomy with ProRoot MTA, RetroMTA, and TheraCal in dogs’ teeth / J Endod 41 : 1317~1324

[journal] Chung CJ / 2016 / Effects of two fast-setting calcium-silicate cements on cell viability and angiogenic factor release in human pulp-derived cells / Odontology 104 : 143~151

[journal] Kim KA / 2015 / Odontogenic effects of a fast-setting calcium-silicate cement containing zirconium oxide / Dent Mater J 34 : 432~440

[journal] Lee S / 2011 / Comparison of mesenchymallike stem/progenitor cells derived from supernumerary teeth with stem cells from human exfoliated deciduous teeth / Regen Med 6 : 689~699

[journal] Kang SH / 2015 / Color changes of teeth after treatment with various mineral trioxide aggregate-based materials : an ex vivo study / J Endod 41 : 737~741

[web] / EZEKIEL : EZ-Seal / http://www.ezekiel.co.kr/03_ezseal/ezseal.html

[journal] Choi Y / 2013 / Biological effects and washout resistance of a newly developed fast-setting pozzolan cement / J Endod 39 : 467~472

[journal] Darvell BW / 2011 / "MTA"-an Hydraulic Silicate Cement : review update and setting reaction / Dent Mater 27 : 407~422

[journal] Min KS / 2007 / The induction of heme oxygenase-1 modulates bismuth oxide-induced cytotoxicity in human dental pulp cells / J Endod 33 : 1342~1346

[journal] Kim EC / 2008 / Evaluation of the radiopacity and cytotoxicity of Portland cements containing bismuth oxide / Oral Surg Oral Med Oral Pathol Oral Radiol Endod 105 : 54~57

[journal] Li Q / 2013 / The impact of zirconium oxide nanoparticles on the hydration chemistry and biocompatibility of white Portland cement / Dent Mater J 32 : 808~815

[journal] 이혜원 / 2015 / Biologic Response of Human Deciduous Dental Pulp Cells on Newly Developed MTA-like Materials / 대한소아치과학회지 / 대한소아치과학회 42(4) : 291~301

[journal] Saw TY / 2005 / Tooth slice organ culture and established cell line culture models for cytotoxicity assessment of dental materials / Toxicol In Vitro 19 : 145~154

[journal] Dawood AE / 2015 / Calcium silicatebased cements: composition, properties, and clinical applications / J Investig Clin Dent 8(2)

[journal] Fridland M / 2005 / MTA solubility : a long term study / J Endod 31 : 376~379

[journal] Torabinejad M / 1995 / Physical and chemical properties of a new root-end filling material / J Endod 21 : 349~353

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