Characterization of Human Dental Pulp Cells from Supernumerary Teeth by Using Flow Cytometry Analysis

Yonsook You (류연숙); Kim Jongbin (김종빈); JISUN SHIN (신지선); June-Haeng Lee (이준행); Jong-Soo Kim (김종수)

doi:10.5933/JKAPD.2019.46.3.337

Characterization of Human Dental Pulp Cells from Supernumerary Teeth by Using Flow Cytometry Analysis

Journal of the Korean academy of pediatric dentistry
Abbr : J Korean Acad Pediatr Dent
2019, 46(3), pp.337-342
DOI : 10.5933/JKAPD.2019.46.3.337
Publisher : The Korean Academy Of Pediatric Dentistry
Research Area : Medicine and Pharmacy > Dentistry
Received : March 27, 2019
Accepted : June 25, 2019
Published : August 31, 2019

Yonsook You ¹, Kim Jongbin ², JISUN SHIN ², June-Haeng Lee ³, Jong-Soo Kim ²

¹미사서울치과
²단국대학교
³단국대학교 치과대학 세종치과병원 소아치과

Accredited

ABSTRACT

The aim of this study was to analyze cells from human dental pulp tissue of impacted supernumerary teeth as stem cells with flow cytometry. Human dental pulp cells from 15 supernumerary teeth were identified their characteristics as stem cells by expression of mesenchymal stem cell markers through flow cytometry analysis at passage 3 and passage 10. Cluster of differentiation (CD) 73, CD 90, CD 34, CD 45 and STRO-1 cell surface markers were used to figure out characteristics of dental pulp stem cells from supernumerary teeth. At passage 3, the cell population showed positive expression of CD 73, CD90 and STRO-1, lacked expression of CD 34 and CD 45. At passage 10, CD 73, CD 90 and STRO- 1 showed positive expression while CD 34 and CD 45 showed negative expression. This study indicated that dental pulp stem cells of supernumerary teeth had the properties of mesenchymal stem cells at both early and late passage. Impacted supernumerary teeth could be considered as a noble source of stem cells because of rapid growth and maintaining characteristics of stem cells until late passage.

KEYWORDS

Supernumerary tooth, Dental pulp stem cell, Flow cytometry

Citation status

* References for papers published after 2023 are currently being built.

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Journal of the Korean academy of pediatric dentistry 2023 KCI Impact Factor : 0.48

Characterization of Human Dental Pulp Cells from Supernumerary Teeth by Using Flow Cytometry Analysis

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* References for papers published after 2023 are currently being built.

Journal of the Korean academy of pediatric dentistry 2023 KCI Impact Factor : 0.48

Characterization of Human Dental Pulp Cells from Supernumerary Teeth by Using Flow Cytometry Analysis

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KEYWORDS

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