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Characterization of Human Dental Pulp Cells from Supernumerary Teeth by Using Flow Cytometry Analysis

  • Journal of the Korean academy of pediatric dentistry
  • Abbr : J Korean Acad Pediatr Dent
  • 2019, 46(3), pp.337-342
  • DOI : 10.5933/JKAPD.2019.46.3.337
  • Publisher : The Korean Academy Of Pediatric Dentistry
  • Research Area : Medicine and Pharmacy > Dentistry
  • Received : March 27, 2019
  • Accepted : June 25, 2019
  • Published : August 31, 2019

Yonsook You 1 Kim Jongbin 2 JISUN SHIN 2 June-Haeng Lee 3 Jong-Soo Kim ORD ID 2

1미사서울치과
2단국대학교
3단국대학교 치과대학 세종치과병원 소아치과

Accredited

ABSTRACT

The aim of this study was to analyze cells from human dental pulp tissue of impacted supernumerary teeth as stem cells with flow cytometry. Human dental pulp cells from 15 supernumerary teeth were identified their characteristics as stem cells by expression of mesenchymal stem cell markers through flow cytometry analysis at passage 3 and passage 10. Cluster of differentiation (CD) 73, CD 90, CD 34, CD 45 and STRO-1 cell surface markers were used to figure out characteristics of dental pulp stem cells from supernumerary teeth. At passage 3, the cell population showed positive expression of CD 73, CD90 and STRO-1, lacked expression of CD 34 and CD 45. At passage 10, CD 73, CD 90 and STRO- 1 showed positive expression while CD 34 and CD 45 showed negative expression. This study indicated that dental pulp stem cells of supernumerary teeth had the properties of mesenchymal stem cells at both early and late passage. Impacted supernumerary teeth could be considered as a noble source of stem cells because of rapid growth and maintaining characteristics of stem cells until late passage.

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