Antimicrobial Effect of Photodynamic Therapy Using Plaque Disclosing Agent (치면착색제를 이용한 광역동 치료의 항균 효과)

Junhee Kho (고준희); Howon Park (박호원); LEE JU HYUN (이주현); Hyunwoo Seo (서현우); Siyoung Lee (이시영)

doi:10.5933/JKAPD.2020.47.2.120

Antimicrobial Effect of Photodynamic Therapy Using Plaque Disclosing Agent

Journal of the Korean academy of pediatric dentistry
Abbr : J Korean Acad Pediatr Dent
2020, 47(2), pp.120~127
DOI : 10.5933/JKAPD.2020.47.2.120
Publisher : The Korean Academy Of Pediatric Dentistry
Research Area : Medicine and Pharmacy > Dentistry
Received : October 2, 2019
Accepted : November 18, 2019
Published : May 29, 2020

Junhee Kho ¹, Howon Park ², LEE JU HYUN ², Hyunwoo Seo ², Siyoung Lee ²

¹강릉원주대학교 치과대학 소아∙청소년치과학교실 및 구강과학연구소
²강릉원주대학교

Accredited

ABSTRACT

The aim of this study was to evaluate the antimicrobial effect of photodynamic therapy (PDT) using plaque disclosing agent, 10 - 20 mM erythrosine, as a photosensitizer. Multispecies cariogenic biofilms containing Streptococcus mutans , Lactobacillus casei and Candida albicans were formed on hydroxyapatite disc. 20 μM, 10 mM and 20 mM erythrosine were applied as a photosensitizer for 3 minutes, and then light-emitting diode (LED) irradiated for 24 seconds. Colony-forming unit (CFU) were measured and biofilms were observed using confocal laser scanning microscopy (CLSM). CFU were significantly decreased in the PDT groups using 10 - 20 mM erythrosine (10 mM, 20mM) and the results were also confirmed by CLSM. This study confirms the high antimicrobial effect of photodynamic therapy using plaque disclosing agent as a photosensitizer.

KEYWORDS

Photodynamic therapy, Erythrosine, Plaque disclosing agent, Multispecies biofilm

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