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Effects of Bee-venom Treatment on the Expression of NOS, C-fos, Serotonin and Substance P in Central Nerve System of Monosodium Iodoacetate-induced Osteoarthritic Pain Model

  • Journal of Korean Medicine Rehabilitation
  • Abbr : JKMR
  • 2007, 17(3), pp.1-18
  • Publisher : The Korean Academy Of Oriental Rehabilitation Medicine
  • Research Area : Medicine and Pharmacy > Korean Medicine

박동수 1 Kim, Soon-joong 1 Seo,Il-bok 1 Jeong, Su-hyeon 1

1세명대학교

Candidate

ABSTRACT

Objectives : This study was carried out to investigate the effects of Bee-venom treatment on the expression of NOS, c-fos, serotonin and substance P in central nerve system of monosodium iodoacetate(MIA)-induced osteoarthritic pain model. Methods : Arthritis was induced by injection of MIA(0.5mg) into knee joint cavities of rats. Arthritic rats were divided into control(n=8) and treated(n=8) group. Control group was injected with normal saline once a day for 20 days, while treated group was injected with Bee-venom extract once a day for same duration. Injected point of Bee-venom and saline was subcutaneous tissue at Tokpi(ST35) acupoint. Histochemical study of NADPH-d and immunohistochemical study of c-fos, serotonin and substance P in brain and spinal cord were performed at 20 days after MIA injection. Results : 1. The numbers of NADPH-d positive cells in superficial dorsal horn of spinal cord of treated group(24±4) was significantly (p<0.01) decreased compared with control(33±5). The numbers of NADPH-d positive cell in dorsolateral periaqueductal gray matter of treated group(114±18) was significantly(p<0.01) decreased compared with control(143±14). 2. The numbers of c-fos positive cells in dorsal periaqueductal gray matter of treated group(56±8) was significantly(p<0.01) decreased compared with control(78±13). The numbers of c-fos positive cells in paraventricular thalamic nucleus of treated group(64±14) was significantly decreased compared with control(88±27). 3. The numbers of serotonin positive cells in median raphe nucleus of treated group(104±15) was significantly(p<0.05) decreased compared with control(139±34). Conclusions : On the basis of these results, we concluded that Bee-venom treatment has inhibiting effects on the pain transmission in monosodium iodoacetate-induced osteoarthritic pain model in rat.

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