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The Study of Naetakcheongeum-san(Neituoqianjin-san) on Anti-Cancer Effects and Mechanisms in Human Leukemic HL-60 Cells

  • Journal of Korean Medicine Rehabilitation
  • Abbr : JKMR
  • 2006, 16(3), pp.49-64
  • Publisher : The Korean Academy Of Oriental Rehabilitation Medicine
  • Research Area : Medicine and Pharmacy > Korean Medicine

김상돈 1 Kwon Kang-Beom 1 Shin, Byung Cheul ORD ID 2 Park Tae Yong 3 Young Dal Kwon 1 SONG YUNG SUN 1 박종하 1

1원광대학교
2부산대학교
3한국외국어대학교

Candidate

ABSTRACT

Objectives : The aim of this study was to investigate the apoptotic effects and mechanisms of Naetakcheongeum-san(Neituoqianjin-san) extract (NCSE)-treated on HL-60 leukemic cells. Methods : It was observed that NCSE induced apoptotic changes in a dose-dependent patterns, which was confirmed by DNA fragmentation and an increase of annexin V+/PI- stained cell population. It was monitored that the activation of caspase-3, -8, -9 enzyme activities and the cleavages of those in NCSE-treated HL-60 cells. We investigated the release of cytochrome c from the mitochondria to the cytosol and the bid cleavage in time- and dose-dependent patterns in HL-60 cells. To clarify the involvement of caspases, we used the inhibitors. Cells treated with the caspase-3 inhibitor, Ac-DEVD-CHO, and the caspase-8 inhibitor, z-IETD-FMK and the caspase-9 inhibitor, z-LEHD-FMK. Results and conclusions : It is suggested that NCSE-induced apoptosis is predominant through the activation of caspase-3 and -8 in HL-60 cells.

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