The Study of Naetakcheongeum-san(Neituoqianjin-san) on Anti-Cancer Effects and Mechanisms in Human Leukemic HL-60 Cells (인간백혈병 세포주에서 내탁천금산의 항종양 효과 및 기전에 관한 연구)

김상돈; Kwon Kang-Beom (권강범); Shin, Byung Cheul (신병철); Park Tae Yong (박태용); Young Dal Kwon (권영달); SONG YUNG SUN (송용선); 박종하

The Study of Naetakcheongeum-san(Neituoqianjin-san) on Anti-Cancer Effects and Mechanisms in Human Leukemic HL-60 Cells

Journal of Korean Medicine Rehabilitation
Abbr : JKMR
2006, 16(3), pp.49-64
Publisher : The Korean Academy Of Oriental Rehabilitation Medicine
Research Area : Medicine and Pharmacy > Korean Medicine

김상돈 ¹, Kwon Kang-Beom ¹, Shin, Byung Cheul ², Park Tae Yong ³, Young Dal Kwon ¹, SONG YUNG SUN ¹, 박종하 ¹

¹원광대학교
²부산대학교
³한국외국어대학교

Candidate

ABSTRACT

Objectives : The aim of this study was to investigate the apoptotic effects and mechanisms of Naetakcheongeum-san(Neituoqianjin-san) extract (NCSE)-treated on HL-60 leukemic cells. Methods : It was observed that NCSE induced apoptotic changes in a dose-dependent patterns, which was confirmed by DNA fragmentation and an increase of annexin V+/PI- stained cell population. It was monitored that the activation of caspase-3, -8, -9 enzyme activities and the cleavages of those in NCSE-treated HL-60 cells. We investigated the release of cytochrome c from the mitochondria to the cytosol and the bid cleavage in time- and dose-dependent patterns in HL-60 cells. To clarify the involvement of caspases, we used the inhibitors. Cells treated with the caspase-3 inhibitor, Ac-DEVD-CHO, and the caspase-8 inhibitor, z-IETD-FMK and the caspase-9 inhibitor, z-LEHD-FMK. Results and conclusions : It is suggested that NCSE-induced apoptosis is predominant through the activation of caspase-3 and -8 in HL-60 cells.

KEYWORDS

Naetakcheongeum-san(Neituoqianjin-san), HL-60 cell, Apoptosis, Caspases

Citation status

* References for papers published after 2023 are currently being built.

[journal] / 1994 / 東西醫學比較硏究 : 31 41~6 4

[journal] / 1995 / Apoptosis in the pathogenesis and treatment of disease / : 267~

[journal] / 2001 / Mitochondrial permeability transition induced by the anticancer drug etoposide Toxicology in Vitro 15 : 265~270

[journal] / 2000 / Anticancer drug-induced apoptosis in human monocytic leukemic cell line U937 requires activation of endonuclease(s) 11(1) : 39~48

[journal] / 2001 / Natural products in anticancer therapy 1(4) : 364~369

[journal] / 1993 / Bleomycin.An apoptosis-mimetic drug that induces two types of cell death depending on the number of molecules internalized 53(22) : 5462~9

[journal] / 1997 / The cell cycle and induction of apoptosis in a hamster fibrosarcoma cell line treated with anti-cancer drugs:its importance to solid tumour chemotherapy 31( ) : 195~207

[journal] / 1999 / 內托千金散加味方이 3-MCA로 誘發된 皮膚癌 및 免疫調節作用에 미치는 影響 12(2) : 20~51

[journal] / 2000 / 內托千金散 및 그 加味方이 마우스의 免疫細胞 및 癌細胞에 미치는 效果 13(1) : 56~

[journal] / 1986 / Rapid colorimetric assay for cell growth and survival modifications to the tetrazolium dye procedure giving improved sensitivity and reliability immu(l) : 271~7

[journal] / 2003 / Induction of apoptosis by takrisodokyeum through generation of hydrogen peroxide and activation of caspase-3 in HL-60 cells 73 : 1895~1906

[journal] / 1980 / 腫瘤的辨證施治 / 上海科學技術出版社 : 1~10

[journal] / 1993 / Programed cell death and the control of cell survival 262 : 695~700

[journal] / 2002 / Death and anti-death: tumour resistance to apoptosis 2 : 277~288

[journal] / 2002 / Apoptosis: a link between cancer genetics and chemotherapy 108 : 153~164

[journal] / 2003 / Herba houttuyniae extract induces apoptotic death of human promyelocytic leukemia cells via caspase activation accompanied by dissipation of mitochondrial membrane potential and cytochrome c release 35(2) : 91~97

[journal] / 1980 / Cell death 68 : 251~306

[journal] / 1998 / Caspase-dependent cleavage of signaling proteins during apoptosis: a turn-off mechanism for anti-apoptotic signals 273(12) : 7141~7147

[journal] / 1996 / Cleavage of huntingtin by apopain, a proapoptotic cysteine protease, is modulated by the polyglutamine tract 13 : 442~449

[journal] / 1997 / Alternative cleavage of Alzheimer-associated presenilins during apoptosis by a caspase-3 family protease 277 : 373~376

[journal] / 1996 / Cleavage of lamin A by Mch2 alpha but not CPP32 multiple interleukin 1 beta-converting enzyme- related proteases with distinct substrate recognition properties are active in apoptosis 93 : 8395~8400

[journal] / 1994 / Cleavage of poly polymerase by a proteinase with properties like ICE : 346~7

[journal] / 1995 / Mice lacking ADPRT and poly (ADP-ribosyl)ation develop normally but are susceptable to skin disease 9 : 509~520

[journal] / 1988 / The mitochondrial death/life regulator in apoptosis and necrosis : 619~42

[journal] / 1997 / The release of cytochrome c from mitochondria: a primary site for bcl-2 regulation of apoptosis 275 : 1132~1136

[journal] / 1997 / Apaf-1, a human protein homologues to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3. 90 : 405~413

[journal] / 1997 / Characterization of Fas (Apo-1, CD95)-Fas ligand interaction 22 : 18827~18833

[journal] / 2002 / Contribution of death receptor and mitochondrial pathways to Fas-mediated apoptosis in the prostatic carcinoma cell line PC3 51(4) : 231~240

[journal] / 1997 / Drug-induced apoptosis in hepatoma cells is medicated by the CD95 (APO-1/Fas) receptor/ligand system and involves activation of wild-type p53 / 99 : 403~413

[journal] / 1997 / The CD95(Apo-1/Fas)system medicated drug induced apoptosis in neuroblastoma cells 57 : 3823~3829

[journal] / 1998 / Chemosensitivity of solid tumor cells in vitro is related to activation of the CD95 system 76 : 105~114

[journal] / 1996 / FLICE: a novel FADD-homologous ICE/CED-3-like protease, is recruited to the CD95 (Fas/APO-1) death--inducing signaling complex 85(6) : 817~827

[journal] / 1997 / Deficient activation of the CD95 (APO-1/Fas) system in drug-resistant cells 11 : 1833~1841

[journal] / 2001 / Shared pathways: death receptors and cytotoxic drugs in cancer therapy 7 : 95~106

[journal] / 2003 / Non-apoptotic functions of caspases in cellular proliferation and differentiation 66 : 1453~1458

[journal] / 1999 / Distinct caspase cascades are initiated in receptor-mediated and chemical-induced apoptosis 274 : 5053~5060

KJCKorea
Journal Central

Journal of Korean Medicine Rehabilitation 2023 KCI Impact Factor : 0.6