Effects of Gamisoyeoum-tang(Jiaweixiaoyan-tang) on the Monosodium Iodoacetate-induced Osteoarthritis in Rats (加味消炎湯이 Monosodium Iodoacetate로 유발된 흰쥐의 골관절염에 미치는 영향)

김용문; Kim， Soon-joong (김순중); Seo,Il-bok (서일복); Jeong， Su-hyeon (정수현)

Effects of Gamisoyeoum-tang(Jiaweixiaoyan-tang) on the Monosodium Iodoacetate-induced Osteoarthritis in Rats

Journal of Korean Medicine Rehabilitation
Abbr : JKMR
2008, 18(1), pp.15-32
Publisher : The Korean Academy Of Oriental Rehabilitation Medicine
Research Area : Medicine and Pharmacy > Korean Medicine

김용문 ¹, Kim， Soon-joong ¹, Seo,Il-bok ¹, Jeong， Su-hyeon ¹

¹세명대학교

Accredited

ABSTRACT

Objectives : This study was to investigate the effects of Gamisoyeoum-tang(Jiaweixiaoyan-tang) on the monosodium iodoacetate-induced osteoarthritis in rats. Methods : Monosodium iodoacetate induced arthritic rats were divided into control and treated group. Control group was taken distilled water for 20 days. Treated group was taken extracts of Gamisoyeoum-tang(Jiaweixiaoyan-tang) by orally for same duration. Normal group was injected with normal saline and was taken distilled water. Body weights were measured at 0, 5, 10, 15, 20 days after injection. At the end of experiment, gross and histopathological examination on the articular cartilages of the knee joints were performed. Contents of TNF-α, IL-1β, IL-6 in synovial fluids and proteoglycan contents of articular cartilages were analysed. COX-2 and iNOS immunohistochemical examination on the knee joints were performed. Results : 1. Body weights of the treated group were significantly increased compared with control group at 20 days after injection. 2. Grossly, the severity of osteoarthritis in the treated group were alleviated compared with control group. 3. PG contents in articular cartilages of the treated group were significantly increased compared with control group. 4. Histopathologically, degenerative and necrotic lesion of articular cartilages in the treated group were alleviated compared with those of the control group. 5. IL-1β contents in synovial fluids of the treated group were significantly decreased compared with control group. 6. Positive reactions of COX-2 in chondrocytes and synovial membranes of the treated group were decreased compared with the control group. Conclusions : On the basis of these results, we concluded that Gamisoyeoum-tang(Jiaweixiaoyan-tang) has anti-arthritic effects on the monosodium iodoacetate-induced osteoarthritis in rats. And it's effects were related with reduced secretion of IL-1β and COX-2 from osteoarthritic chondrocytes and synovial membranes.

KEYWORDS

Monosodium iodoacetate, Osteoarthritis, Proteoglycan, TNF-α, IL-1β, COX-2, Gamisoyeoum-tang(Jiaweixiaoyan-tang)

Citation status

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