Anti-inflammatory Effect of Mugi-hwan Water Extract in RAW 264.7 Cells (戊己丸의 RAW 264.7 세포에 대한 항염증작용 연구)

김일현; 최종환; 이세원; SONG YUNG SUN (송용선)

Anti-inflammatory Effect of Mugi-hwan Water Extract in RAW 264.7 Cells

Journal of Korean Medicine Rehabilitation
Abbr : JKMR
2013, 23(3), pp.27-35
Publisher : The Korean Academy Of Oriental Rehabilitation Medicine
Research Area : Medicine and Pharmacy > Korean Medicine

김일현 ¹, 최종환 ¹, 이세원 ¹, SONG YUNG SUN ²

¹원광대학교 한의과대학 한방재활의학과교실
²원광대학교

Accredited

ABSTRACT

Objectives The aim of this study was to investigate anti-inflammatory activity ofMugi-hwan (MGH) Water Extract. Methods Cells were treated with 2 ug/ml of LPS 1 hour prior to the addition of MGH. Cell viability was measured by MTS assay. The production of NO was determined by reactingcultured medium with Griess reagent. The expression of COX-2, iNOS and MAPKswas investigated by Western blot, RT-PCR. The content of level of cytokines (PGE2, IL-6,in media from LPS-stimulated Raw 264.7 cells was analyed by ELISA kit. Results MGH inhibited the production of NO, PGE2, IL-6 as well as the expressions ofiNOS, COX-2 in the murine macrophage, RAW 264.7 cells. MGH also had suppression effectsof LPS induced MAPKs activation. Conclusions These results suggest that MGH has an anti-inflammatory therapeutic potential,which may result from inhibition of MAPK phosphorylation, thereby decreasing theexpression of pro-inflammatory genes.

KEYWORDS

Mugi-hwan, MAPKs, Anti-inflammatory activity

Citation status

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