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Effects of Saengkanggamchotang (SKT) on MIA-Induced Osteoarthritis in Rats

  • Journal of Korean Medicine Rehabilitation
  • Abbr : JKMR
  • 2013, 23(4), pp.23~37
  • Publisher : The Korean Academy Of Oriental Rehabilitation Medicine
  • Research Area : Medicine and Pharmacy > Korean Medicine

최보미 1,  Minseok Oh 1

1대전대학교

Accredited

ABSTRACT

Objectives This study was performed to investigate the effects of Saengkanggamchotang(SKT) on the monosodium iodoacetate (MIA) induced osteoarthritis in rats. Methods Osteoarthritis was induced by injection of MIA (50 ul, 60 mg/ml) into kneejoints of rats. Rats are divided into a total of 4 groups (normal, control, positive comparisongroup, SKT treated group, each n=6). Normal group are not treated at all without inducingosteoarthritis whereas control group were induced for osteoarthritis by MIA and oral medicatedwith 20 ml of distilled water per day. Positive comparison group was injected withMIA and after 7 days, that was taken indomethacin (30 mg/kg/mouse). SKT treated groupwas injected with MIA and after 7 days that was taken SKT (30 mg/kg/mouse). Positivecomparison group and SKT treated group were oral medicated for each substance a totalof 4 weeks with one time per day. After experiments (from 1 week after injection of papainto 4 weeks elapsed), the functions of liver and kidney, Prostaglandin E2, inflammatory cytokine(IL-1β, IL-6, TNF-α), osteocalcin, TIMP-1, MMP-9 within serum. Knee joint structureswere observed by H&E, safranin-O staining method, and amount of cartilage weremeasured by μCT-arthrography. Results 1) Hind paw weight bearing ability was significantly improved. 2) Functions of liverand kidney were not affected. 3) Prostaglandin E2, osteocalcin, TIMP-1, MMP-9 in serumwere significantly decreased. 4) Inflammatory cytokine IL-1β was significantly decreased,and IL-6, TNF-α were decreased but had not significant. 5) In terms of histopathology,significantly reduced subsidence of cartilage and bone in H&E staining. And inSafranin O staining, proteoglycan content in synovial membrane was significantly increasedcompared with control group. 6) Destruction of cartilage on μCT-arthrographywas significantly reduced. Conclusions Based on all results mentioned above, Saengkanggamchotang (SKT) isbelieved to be meaningful for suppressing the progress of osteoarthritis and its treatments.

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