Journal of Korean Medicine Rehabilitation 2023 KCI Impact Factor : 0.6
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pISSN : 1229-1854
- https://journal.kci.go.kr/jkmr
KJCKorea
Journal Central
Anti-oxidation and Anti-inflammatory Effect of Asiasari Radix in RAW 264.7 Cells
- Journal of Korean Medicine Rehabilitation
- Abbr : JKMR
- 2014, 24(3), pp.99-110
- Publisher : The Korean Academy Of Oriental Rehabilitation Medicine
- Research Area : Medicine and Pharmacy > Korean Medicine
이옥진 1, Minseok Oh 2
1대전대학교 한의과대학 한방재활의학과교실
2대전대학교
Accredited
ABSTRACT
Objectives The purpose of this study was to investigate the Anti-oxidation and anti-inflammatory effects of ethanol extract from asiasari radix (AR) on lipopolysaccharide (LPS)-induced in RAW 264.7 Cells Methods Anti-oxidative effects of AR were measured by scavenging activities of 1,1-diphenyl- 2-picryl-hydrazyl (DPPH), 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and production of reactive oxygen species (ROS) in RAW 264.7 cells. Anti-inflammatory effects of AR were measured by mediators including nitric oxide(NO), interleukin- 1β (IL-1β), interleukin-6 (IL-6), tumor necosis factors-α (TNF-α) and iNOS, IL-1β, IL-6, TNF-α mRNA expression in RAW 264.7 cells.
Results Total phenolic content was expressed 28.77±1.67. DPPH radical Scavenging was increased depend on AR ethanol extract. ABAT radical Scavenging was increased depend on AR ethanol extract. Production of ROS was significantly decreased by AR ethanol extract on concentration of 100 (μg/ml). Production of NO was significantly decreased by AR ethanol extract on concentration of 100 (μg/ml). Production of IL-1β, interleukin-6 and TNF-α were increased depend on AR ethanol extract. And Production of interleukin- 6, TNF-α were significantly decreased AR ethanol extract. iNOS, IL-1β, IL-6, TNF- α mRNA expression of RAW 264.7 cells was increased depend on AR ethanol extract.
Conclusions According to this study, AR ethanol extract has anti-oxidative and anti-inflammatoy effects.
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