2014, Vol.24, No.3

Acupuncture Effects of Fatty Mice Induced by High Fat Diet

Chang-Hoon Woo , Yong Eun Lee , Seong-Soo Roh and 1 other persons | 2014, 24(3) | pp.1~9 | number of Cited : 1

Effect of Mixed Extract of Ephedrae Sinica and Fibrosum Gypsum on Differentiation of Preadipocytes and Obesity of Rats

기영범 , 김대훈 , Mi-Sung Yang and 3 other persons | 2014, 24(3) | pp.11~27 | number of Cited : 3

Objectives This study was designed to evaluate the effects of Ephedra Sinica and Fibrosum Gypsum extract on obesity by using 3T3-L1 cells and high fat diet rats. Methods In vitro, Ephedra Sinica and Fibrosum Gypsum extract (50, 100, 200, 500 μg/ ml) were added in 3T3-L1 cells. Cytotoxicity was measured by MTT assasy. Adipocyte differentiation was measured by Oil Red O staining, GPDH activity and C/EBPα protein expression. In vivo, Sprague-Dawley rats were divided into 5 groups : Normal diet group (Normal group), taken high fat diet and no treatment group (Control group), taken high fat diet and orally administered Ephedra Sinica and Fibrosum Gypsum extract daily (Group I: 50 mg/kg, Group II: 100 mg/kg, Group III : 200 mg/kg, oral). For 6 weeks of administration, body weight and the amount of food intake were measured once a week. After administration, blood analysis (AST, ALT, T-Bilirubin, BUN, RBC, Hb, HCT), serum lipid level (triglyceride, Total cholesterol, HDL, LDL), serum leptin level, epididymal adipose tissue weight and histological finding of liver were estimated. Results In vitro, The cytotoxicity was not significant. 3T3-L1 cell’s differentiation was significantly decreased in Oil Red O staining, GPDH activity and C/EBPα protein expression. In vivo, Body weight and the amount of food intake, AST, ALT, Total cholesterol, TG, LDL, serum leptin, epididymal adipose tissue weight showed significant decrease in group I, group II and group III. There were no significant difference in T-bilirubin, BUN, RBC, Hb and HCT between all groups. HDL showed significant increase in group I, group II and group III. In histological finding of liver tissue, there were decreased adiposity and cytopathic effect in group I, group II and group III. Conclusions It is suggested that Ephedra Sinica and Fibrosum Gypsum extract can be used in the treatment of obesity.

Effects of Yoohyangheukho-dan (Rǔxiānghēihǔ-dān) on Osteoporosis Induced by Ovariectomy in Rats

금창준 , 조창영 , Jeong Min Lim and 4 other persons | 2014, 24(3) | pp.29~37 | number of Cited : 5

Objectives Osteoporosis is an impending problem in the rapidly aging society. The purpose of this study is to identify the effects of Yoohyangheukho-dan (Rǔxiānghēihǔ-dān) on osteoporosis induced by ovariectomy in rats. Methods 24 rats were randomly assigned to a SHAM group, a control group, and a Yoohyangheukho-dan (Rǔxiānghēihǔ-dān) group (n=8). Ovaries were extracted and for 8 weeks, the rats were given dry feeds and Yoohyangheukho-dan (Rǔxiānghēihǔ-dān) group were given a mixture of dry feeds and Yoohyangheukho-dan (Rǔxiānghēihǔ-dān). At 8 weeks, their body weight, femur mass, tibia ash per body ratio, overall surface area and thickness of the trabeculae, overall surface area of the osteoblasts, and the number of osteoclasts were measured and levels of albumin, aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), total-cholesterol, calcium and estradiol were recorded. Results There was no significant difference in the weight of the femur and the tibia ash per body ratio. The Yoohyangheukho-dan (Rǔxiānghēihǔ-dān) group had significantly thicker trabeculae than the control group and showed a minimal increase of overall surface area of the trabeculae. The overall surface area of the osteoblasts and the number of osteoclasts decreased in the Yoohyangheukho-dan (Rǔxiānghēihǔ-dān) group. There were no statistically significant differences in AST, ALT, ALP, total cholesterol, phosphorus, and estradiol levels. On the contrary the Yoohyangheukho-dan (Rǔxiānghēihǔ-dān) group had significantly higher levels of Albumin, triglyceride, and calcium. Conclusions It can be concluded that oral intake of Yoohyangheukho-dan (Rǔxiānghēihǔ- dān) prevents the thinning process of the trabeculae. Thus, it may have positive effects on the treatment and prevention of osteoporosis in postmenopausal women.

Protective Effects of Banggi-eum (FangchiYin) on the Articular Cartilage Injuries Induced by Monosodium Iodoacetate in Rats

정해창 , Jeong， Su-hyeon , Seo,Il-bok | 2014, 24(3) | pp.39~50 | number of Cited : 1

The Effects of Sibjeondaebotanggamibang on the Treating of Wound

정훈 , 이현재 , 김빛나라 and 4 other persons | 2014, 24(3) | pp.51~69 | number of Cited : 7

Objectives This study was aimed to investigate the effects of Sibjeondaebotanggamibang (SJT) on the wound-induced rats. Methods ＜In vitro＞ It was observed the effects of anti-oxidation and anti-inflammation by using of lipopolysaccharide (LPS)-treated RAW 264.7 cells. For the observing on SJT anti-oxidation, it needed to mesure the total amount of polyphenol, DPPH scavenging ability, ABTS scavenging ability and the value of ROS production. In order to observe on the anti-inflammation of SJT, it was mesured the value of No and Cytokine (TNF-α, IL-1β, IL-6). ＜In vivo＞ It needed to make a scar (around 2x2 cm2) on the top of the fascia in the back of the rats and then the rats were divided into 4 groups (n=6). Control group was not treated at all, whereas SJ group was orally medicated SJT, Terra group was percutaneously applied Terramycin, and SJ+Terra group was both orally medicated SJT and percutaneously applied Terramycin per day for three weeks. The size of wound was measured with Digimatic Caliper and the blood samples (WBC, neutrophil, monocyte, lymphocyte) were analyzed using Minos-ST, which were collected by cardiac puncture. The effect on inflammatory cytokine (TNF-α, IL-1β, IL-6), immunological cells in synovial fluid was measured. To measure the wound factor expressed by wounded skin sample, we extracted RNA and to investigate MMP-1,2,9 we used RT-PCR. For performing histopathological examinations, we paralyzed the rats by ether, and extracted wounded skin tissues, which were measured by H & E, and monitored on the optical microscope. Results ＜In vitro＞ 1. DPPH and ABTS scavenging activity of SJT was increased concentration- dependantly, and ROS scavenging activity was significantly increased (10, 100 μg/ ml). 2. NO production was significantly reduced in SJT treated cells (100 μg/ml), both TNF-α and IL-6 in SJT treated cells (1, 10, 100 μg/ml), and IL-1β in SJT treated cells (1, 100 μg/ml). ＜In vivo＞ 1. The size of wound was significantly decreasing in SJ group, Terra group, SJ+Terra group. 2. WBC was significantly reduced in SJ and SJ+Terra group, monocyte in SJ+Terra group. Neutrophil was also reduced in SJ, SJ+Terra group but meaningless. 3. TNF-α and IL-6 were significantly reduced in SJ group, Terra group, SJ+Terra group, and IL-1β in SJ+Terra group. 4. mRNA expression in MMP-1 was significantly reduced in SJ group. 5. Collegan production and chronic inflammation were significantly decreased in SJ group, Terra group, SJ+Terra groups. Re-epithelization on the skin in Terra group, SJ+Terra groups was decreased. Conclusions According to this in vitro experiment, Sibjeondaebotanggamibang (SJT) has the effects of anti-oxidative and anti-inflammatory. By in vivo experiment, SJT has the effects of anti-inflammatory. Moreover, the progress of recovery was found visually, heamatologically, genetically and histopathologically. In conclusion, it could be thought that SJT has effect on the treating of wound.

Anti-allergic Effects of Samhwangsasim-tang (Sānhuángxièxīntāng) on Ovalbumin-induced Allergic Model in Mice

최종환 , 금선오 , 이세원 and 3 other persons | 2014, 24(3) | pp.71~85 | number of Cited : 0

Objectives In this study, we investigated the inhibitory effects of Samhwangsasim-tang (S.H) on the allergic response caused by ovalbumin(OVA) sensitization and challenge in BALB/c mice. Methods The experimental animals were divided into five groups; 1) normal as negative control, 2) OVA-sensitized mice, 3) OVA-sensitized mice treated with 200 mg/kg of S.H 200, 4) OVA-sensitized mice treated with 400 mg/kg of S.H 400, and 5) OVA-sensitized mice treated with 5 mg/kg of Dexamethasone (Dex). Antigen sensitization for allergic mouse model was performed with twice injection of OVA for 2 weeks. After secondary injection, S.H was administrated orally into mice every day for 13 days and the inhibitory effect of S.H on allergic responses was evaluated. Results Treatment of S.H into allergic mice reduced significantly ear edema and infiltration of immune cells in ear tissues induced with OVA challenge in a dose-dependent manner. S.H reduced significantly the serum levels of Total Immunoglobulin(Ig)G and IgE, and particularly inhibited the production of OVA-specific IgE, but not OVA-specific IgG. The serum level of proinflammatory cytokine TNF-α and Th2-associated cytokine IL-4 also were significantly decreased by S.H adminstration in a dose denpendent manner. S.H attenuated OVA-induced secretion of IFN-γ, but not IL-12 which is a cytokine inducing the development of Th1 cells. It also reduced significantly the secretion of IL-4, which is a cytokine inducing the development of Th2 cells, after splenocytes were stimulated with OVA. However the secretion of IL-5 and IL-13 was influenced weakly or a little. Conclusions These results indicate that S.H could reduce the allergic response through inhibition of antigen-specific IgE and Th2-inducing cytokines. It suggest that S.H may be available clinically for the treatment of allergic patients.

Therapeutic Effects of Acupuncture and Herbal External Preparation on Healing of Deep Partial Thickness Burn Wound in Rats

Jo Hee Geun , 박애련 , choi jin bong | 2014, 24(3) | pp.87~98 | number of Cited : 2

Objectives The present study aimed to investigate the effects of acupuncture and herbal external preparation on wound healing procedure in a deep partial thickness burn model in rats. Methods A total of 40 male Sprague-Dawley rats were allocated into four groups with an equal number in each group: acupuncture treatment group, herbal external perparation group, Silver sulfadiazine dressing group and control group. We describe the effect of acupuncture and herbal external preparation on morphologic and histologic changes, epithelial growth factor (EGF), hematological value of the deep partial thickness burn wound in rats. Results At 21th day after wounding the wound size in acupuncture treat group and herbal external preparation group were decreased more significantly compared to control group. In addition, epidermal regeneration on acupuncture treatment was than other treatment and control group in histological finding. Hematological findings revealed that acupuncture treatment group and herbal external prepartion group was more effective than control group in reducing inflammation response induced by burn. In acupuncture treated group, neutrophil and leukocyte level were significantly decreased compared to other treatment group. Also, this study showed that EGF was obviously expressed in nascent tissue when wounds were treated with the acupuncture and herbal external preparation after injury. In particular, acupuncture treatment group had a significant increase of EGF expression in burn wound healing area when compared with the other treatment groups. Conclusions These findings suggest that acupuncture and herbal external prepration may improve burn wound healing through decreasing inflammatory reaction, increasing tissue regeneration and expression of EGF. Moreover, acupuncture treatment could be more effective in comparison with Silver sulfadiazine dressing.

Anti-oxidation and Anti-inflammatory Effect of Asiasari Radix in RAW 264.7 Cells

이옥진 , Minseok Oh | 2014, 24(3) | pp.99~110 | number of Cited : 4

Thermal Distribution in Living Tissue during Warm Needling Therapy

김종연 , 이종수 | 2014, 24(3) | pp.111~119 | number of Cited : 6

The Effects of MET and ICT in Patients with Lumbago by Meridian Muscle Electrography

조동인 , 박동수 , Jeong， Su-hyeon and 1 other persons | 2014, 24(3) | pp.121~130 | number of Cited : 4

Comparison of Effects between MSAT on Piriformis Muscle and Non-MSAT on Piriformis Muscle in Patients with HLD with Leg Radiating Pain: A Retrospective Analysis

허석원 , 최철우 , 이동현 and 5 other persons | 2014, 24(3) | pp.131~137 | number of Cited : 3

Objectives The purpose of this study was to compare the effects of MSAT (Mostion style acuputure treatment) on piriformis muscle regarding leg radiating pain with HIVD of L-SPINE. Methods Forty patients who received inpatient treatment from Febuarary 1 to March 31, 2014 in the Daejon-Jaseng oriental medicine hospital were divided into 2 groups by blocked randomization : Group A (n=20)=routine treatment on HIVD of L-SPINE with MSAT on piriformis muscle and Group B (n=20)=routine treatment on HIVD of L-SPINE (acupuncture, Shinbaro pharmacopuncture, herb medication, chuna treatment and physiotherapy). The MSAT (Mostion style acuputure treatment) on piriformis muscle was administered each day and both groups were received routine treatment each day. Evaluations were made before treatment, after 3 days and 7 days, and before discharge using the NRS (numeric rating scale) and ODI (oswestry disability index). The statistically significance was evaluated by SPSS 18.0 for windows. Results Compared to before treatment, the NRS (numeric rating scale) and ODI (oswestry disability index) of both A and B groups after 3, 7 days and before discharge of treatment significantly decreased (p＜0.05). Regarding group comparison, the NRS (numeric rating scale) and ODI (oswestry disability index) of A group was significantly lower than the B group at 3, 7 days, before dischanre (p＜0.05). Regarding improvement of the NRS (numeric rating scale) and ODI (oswestry disability index), A group was significantly lower than the B group at 3 day (NRS), 7day (ODI) (p=0.000) but there was no significant difference between the two groups at 7 days (NRS) and before discharge (p＞0.05). Conclusions Compared to just routine treatment, the MSAT (Mostion style acuputure treatment) on piriformis muscle significantly improved the NRS (numeric rating scale) and ODI (oswestry disability index) after 3, 7 days, and before discharge. Because early intervention determines the grade of Leg radiating pain with HIVD of L-SPINE, the MSAT (Mostion style acuputure treatment) on piriformisat the early stage will be clinically helpful to patients.

A Retrospective Study about the Relationship of Improvement of Low Back Pain and Lumbosacral-Pelvic Parameters

Cho Sungwoo | 2014, 24(3) | pp.139~148 | number of Cited : 3

Study on Correlation between Difference of Pelvic Height and Gait Balance of Patients with Abnormal Postures

박정우 , Jeong， Su-hyeon | 2014, 24(3) | pp.149~155 | number of Cited : 5

The Comparative Study on the Effects of Chuna and Combined with Acupuncture in Patients with Dysmenorrhea

조동인 , 류성진 , 남은영 and 3 other persons | 2014, 24(3) | pp.157~164 | number of Cited : 8

The Study on Pillow Height for Arm Paresthesia Patients

권헌준 , 김정섭 , 이종훈 and 2 other persons | 2014, 24(3) | pp.165~171 | number of Cited : 2

A Clinical Case of Muscle Energy Technique and Schroth Method for Idiopathic Scoliosis

이성준 , 박재현 , 남수현 and 1 other persons | 2014, 24(3) | pp.173~179 | number of Cited : 2

Sudden Hearing Loss Treated by Chuna Manual Therepy; A Case Report

김성태 , 이은지 , 한세혁 and 8 other persons | 2014, 24(3) | pp.181~186 | number of Cited : 2

KJCKorea
Journal Central

Journal of Korean Medicine Rehabilitation 2023 KCI Impact Factor : 0.6

2014, Vol.24, No.3