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@article{ART002580657},
author={Jung-Oh Park and Minseok Oh},
title={The Healing Effect of Jinmu-tang (Zhenwu-tang) in Femur Fractured Rats},
journal={Journal of Korean Medicine Rehabilitation},
issn={1229-1854},
year={2020},
volume={30},
number={2},
pages={19-35},
doi={10.18325/jkmr.2020.30.2.19}

TY - JOUR
AU - Jung-Oh Park
AU - Minseok Oh
TI - The Healing Effect of Jinmu-tang (Zhenwu-tang) in Femur Fractured Rats
JO - Journal of Korean Medicine Rehabilitation
PY - 2020
VL - 30
IS - 2
PB - The Korean Academy Of Oriental Rehabilitation Medicine
SP - 19
EP - 35
SN - 1229-1854
AB - Objectives The aim of this study is to evaluate the fracture healing effect of Jinmu-tang (JM) on femur fractured rats. Methods Rats were randomly divided into 5 groups (normal, control, positive control, JM extract with low concentration and JM extract with high concentration). All group except normal group went through both femur fracture. Normal and control group received no treatment at all. Positive control group were medicated with tramadol (20 mg/kg) once a day for 14 days. Experimental group was orally medicated with JM extract (10 mg/kg for low concentration, 50 mg/kg for high concentration) once a day for 14 days. In order to investigate fracture healing process, plasma and serum were obtained. Also, micro-computed tomography was conducted to see the frature site visually. Immunohistochemistry for transforming growth factor-β1, Ki67, alkaline phosphatase, runt-related transcription factor 2, receptor activator of nuclear factor kappa-β, tartrate resistant acid phosphatase was conducted to observe bone healing progress after 14 days since fracture occured. Aspartate aminotransferase, alanine aminotransferase, blood urea nitrogen and creatinine levels were measured in plasma, for hepatotoxicity and nephrotoxicity of JM extract. Osteocalcin was measured to observe activity of osteoblast. Results Through Micro-CT, more fracture healing was observed on both experimental group than control and positive control group. Through Hematoxylin & Eosin and safranin O staining showed bone cell proliferation and bone formation in the experimental group. RANK was significantly increased in the experimental groups. JM with high concentration showed statistically significant of TGF-β and Osteocalcin. NO, TRAP and ALP were not significantly changed. Liver toxicity was not significantly observed. Creatinine significantly increased in both experimental groups after 28 days. Conclusions As described above, JM extract showed anti-inflammatory effect, promoted fracture healing by stimulating the bone regeneration factor, and showed little hepatotoxicity and nephrotoxicity. In conclusion, JM extract can promote fracture healing and it can be used clinically to patients with fracture. (J Korean Med Rehabil 2020;30(2):19-35)
KW - Herbal medicine;Bone fractures;Fracture healing;Gene expression
DO - 10.18325/jkmr.2020.30.2.19
ER -

Jung-Oh Park and Minseok Oh. (2020). The Healing Effect of Jinmu-tang (Zhenwu-tang) in Femur Fractured Rats. Journal of Korean Medicine Rehabilitation, 30(2), 19-35.

Jung-Oh Park and Minseok Oh. 2020, "The Healing Effect of Jinmu-tang (Zhenwu-tang) in Femur Fractured Rats", Journal of Korean Medicine Rehabilitation, vol.30, no.2 pp.19-35. Available from: doi:10.18325/jkmr.2020.30.2.19

Jung-Oh Park, Minseok Oh "The Healing Effect of Jinmu-tang (Zhenwu-tang) in Femur Fractured Rats" Journal of Korean Medicine Rehabilitation 30.2 pp.19-35 (2020) : 19.

Jung-Oh Park, Minseok Oh. The Healing Effect of Jinmu-tang (Zhenwu-tang) in Femur Fractured Rats. 2020; 30(2), 19-35. Available from: doi:10.18325/jkmr.2020.30.2.19

Jung-Oh Park and Minseok Oh. "The Healing Effect of Jinmu-tang (Zhenwu-tang) in Femur Fractured Rats" Journal of Korean Medicine Rehabilitation 30, no.2 (2020) : 19-35.doi: 10.18325/jkmr.2020.30.2.19

Jung-Oh Park; Minseok Oh. The Healing Effect of Jinmu-tang (Zhenwu-tang) in Femur Fractured Rats. Journal of Korean Medicine Rehabilitation, 30(2), 19-35. doi: 10.18325/jkmr.2020.30.2.19

Jung-Oh Park; Minseok Oh. The Healing Effect of Jinmu-tang (Zhenwu-tang) in Femur Fractured Rats. Journal of Korean Medicine Rehabilitation. 2020; 30(2) 19-35. doi: 10.18325/jkmr.2020.30.2.19

Jung-Oh Park, Minseok Oh. The Healing Effect of Jinmu-tang (Zhenwu-tang) in Femur Fractured Rats. 2020; 30(2), 19-35. Available from: doi:10.18325/jkmr.2020.30.2.19

Jung-Oh Park and Minseok Oh. "The Healing Effect of Jinmu-tang (Zhenwu-tang) in Femur Fractured Rats" Journal of Korean Medicine Rehabilitation 30, no.2 (2020) : 19-35.doi: 10.18325/jkmr.2020.30.2.19