2014, Vol.14, No.2

Journal of Korean Medicine for Obesity Research 2023 KCI Impact Factor : 0.43

pISSN : 1976-9334 / eISSN : 2288-1522
https://journal.kci.go.kr/jkomor

Objectives: This study is to confirm the effect of combined extract and individual extract ofSamjunghwan (SJH) in anti-oxidative and anti-obesity effect. Methods: Combined ethanol extract of readily made SJH and individual ethanol extract ofAtractylodes japonica, Cortex lycii radicis, and Morus alba Linne was combined after theextraction. To evaluate the anti-oxidative effect of SJH, total phenol compound and2,2-Diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging ability were conducted. Real-timequantitative-polymerase chain reaction analysis of transcription factor peroxisomeproliferator-activated receptror γ (PPARγ), adenosine monophosphate-activated protein kinase(AMPK)-α1, tumor necrosis factor-α (TNFα) and 3-hydroxy-3-methylglutaryl CoA reducatase(HMG-CoA reductase) were done with 3T3-L1 cells to investigate the ant-obesity effect. Also,cell viability analysis were done to see to toxicity of SJH. Results: Individual extract of SJH showed significant decrease in TNFα and AMPK transcriptionwhile PPARγ showed significant increase. Combined extract and individual extract of SJH bothshowed decrease in HMG-CoA reductase. DPPH free radical scavenging ability and total phenolcompound was analogous between two groups. Conclusions: Individual extract of SJH appears to be more effective in anti-oxidation andanti-obesity effect compared to combined extract of SJH.

Mitochondrial dysfunction is known to be involved in insulin resistance and obesity, researcheshave been increasing highly. Astragali Radix extract (ARE) or its main components have beenshown to perform comparably to insulin by significantly reducing blood glucose levels in animalmodels however, the influence on mitochondrial dysfunction are not well understood. Methods: ARE (0.2, 0.5 and 1.0 mg/ml) or metformin (2.5 mM) were treated in C2C12 after 6day-differentiation. The expressions of adenosine monophosphate (AMP)-activated proteinkinase (AMPK) and phosphorylation AMPK, peroxisome proliferators-activated receptror γcoactivator 1α (PGC1α), nuclear respiratory factors 1 (NRF1), mitochondrial transcription factor(Tfam) and myosin heavy chain were detected with western blotting or polymerase chain reactionanalysis. The morphological changes were also investigated. Results: ARE dose dependently increased phosphorylation of AMPK and respectively activatedmRNA expressions of PGC1α, NRF1 and Tfam which are mitochondrial biogenesis regulators. Furthermore, there were some morphologic differences of differentiated cells between AREtreatment and control. Conclusions: This study suggests that ARE has the potential to increase muscle mitochondrialfunction by activating AMPK and PGC1α.

Objectives: This study was designed to investigate the correlation between Sasang constitutionand dyslipidemia and the possibility that Sasang constitution can be a risk factor of dyslipidemia. Methods: This study was a retrospective cross-sectional study based on chart review. Seventeenthousand sixty-nine charts which were collected from May 2007 to June 2010 in InternationalHealthcare Service Center, Kyung Hee University Medical Center, were reviewed for the study. Fifteen thousand two hundred fifty charts including Sasang constitutional diagnosis and lipidprofiling were selected and we examined the correlation between Sasang constitution anddyslipidemia. Results: After adjusted for body mass index, the levels of total cholesterol and triglyceride werehighest in Taeeum group, and lowest in Soeum group. High-density lipoprotein was highest inSoeum group, and lowest in Taeeum group. After adjusted for age, sex, and body mass index, theodds ratios for ‘high triglyceride’ were 1.716 (1.411∼2.087) in Soyang group and 2.021 (1.650∼2.475) in Taeeum group compared to Soeum group. The odds ratio for ‘high low-densitylipoprotein’ was 1.229 (1.006∼1.501) in Soyang group compared to Soeum group. The oddsratios for ‘low high-density lipoprotein’ were 1.195 (1.033∼1.381) in Soyang group and 1.414(1.212∼1.649) in Taeeum group compared to Soeum group. Conclusions: The lipid profiles and dyslipidemic risk differed across constitutional types, showingconstitution type-specific patterns, so that we could infer the possibility that Sasangconstitutional types can be a risk factor of dyslipidemia.