Anti-Oxidative and Anti-Obesity Effect of Combined Extract and Individual Extract of Samjunghwan (혼합추출 및 개별추출 방식의 삼정환의 항산화 및 항비만효과)

Kyungsun Han (한경선); Jing-Hua Wang (WANG JINGHUA); Dongwoo Lim (임동우); CHIN, YOUNG-WON (진영원); Choi, Young-Hee (최영희); Choi, Han-Seok (최한석); Myeong-Jong Lee (이명종); Hojun Kim (김호준)

Anti-Oxidative and Anti-Obesity Effect of Combined Extract and Individual Extract of Samjunghwan

Journal of Korean Medicine for Obesity Research
Abbr : J Korean Med Obes Res
2014, 14(2), pp.47-54
Publisher : The Society of Korean Medicine for Obesity Research
Research Area : Medicine and Pharmacy > Korean Medicine
Received : November 15, 2014
Accepted : December 4, 2014
Published : December 30, 2014

Kyungsun Han ¹, Jing-Hua Wang ², Dongwoo Lim ³, CHIN, YOUNG-WON ⁴, Choi, Young-Hee ⁴, Choi, Han-Seok ⁴, Myeong-Jong Lee ⁴, Hojun Kim ⁴

¹동국대학교 일산한방병원 한방재활의학과
²동국대학교(경주캠퍼스) 한의학연구소
³동국대학교 한의과대학 한방병리학교실
⁴동국대학교

Candidate

ABSTRACT

Objectives: This study is to confirm the effect of combined extract and individual extract ofSamjunghwan (SJH) in anti-oxidative and anti-obesity effect. Methods: Combined ethanol extract of readily made SJH and individual ethanol extract ofAtractylodes japonica, Cortex lycii radicis, and Morus alba Linne was combined after theextraction. To evaluate the anti-oxidative effect of SJH, total phenol compound and2,2-Diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging ability were conducted. Real-timequantitative-polymerase chain reaction analysis of transcription factor peroxisomeproliferator-activated receptror γ (PPARγ), adenosine monophosphate-activated protein kinase(AMPK)-α1, tumor necrosis factor-α (TNFα) and 3-hydroxy-3-methylglutaryl CoA reducatase(HMG-CoA reductase) were done with 3T3-L1 cells to investigate the ant-obesity effect. Also,cell viability analysis were done to see to toxicity of SJH. Results: Individual extract of SJH showed significant decrease in TNFα and AMPK transcriptionwhile PPARγ showed significant increase. Combined extract and individual extract of SJH bothshowed decrease in HMG-CoA reductase. DPPH free radical scavenging ability and total phenolcompound was analogous between two groups. Conclusions: Individual extract of SJH appears to be more effective in anti-oxidation andanti-obesity effect compared to combined extract of SJH.

KEYWORDS

Samjunghwan, Obesity, Antioxidants

Citation status

* References for papers published after 2023 are currently being built.

[confproc] Grundy SM / 2004 / Definition of metabolic syndrome report of the National Heart, Lung, and Blood Institute/American Heart Association Conference on scientific issues related to definition / Circulation 109(3) : 433~438

[journal] Hotamisligil GS / 1999 / The role of TNFα and TNF receptors in obesity and insulin resistance / J Intern Med 245(6) : 621~625

[journal] 문근아 / 2003 / Human and Animal Study on the Natural Food for Obesity and Metabolic Syndrome Risk Factors / 한국식품영양과학회지 / 한국식품영양과학회 32(8) : 1394~1400

[journal] 김동웅 / 2009 / Study on the Changes in the Blood Lipid Profile Levels of Patients with Metabolic Syndrome while Receiving Oriental Medicine Treatments for Various Diseases / 동의생리병리학회지 / 대한동의병리학회 23(2) : 512~519

[journal] Kim YC / 2005 / antioxidant properties of flavone C‐glycosides from atractylodes japonica leaves in human low‐density lipoprotein oxidation / J Food Sci 70(9) : S575~S580

[journal] Gao D / 2007 / Hypoglycemic effects and mechanisms of action of Cortex Lycii Radicis on alloxan-induced diabetic mice / Yakugaku Zasshi 127(10) : 1715~1721

[journal] 김애정 / 2008 / Mulberry Fruit Extract Consumption is Inversely Associated with Hyperlipidemia in Middle-aged Men / 한국식품영양학회지 / 한국식품영양학회 21(2) : 121~126

[journal] 김세윤 / 2006 / Effects of Samjunghwan on the IL-1β Gene Expression in the Macrophage / 대한한방내과학회지 / 대한한방내과학회 27(1) : 228~236

[journal] Kim HG / 2010 / Evaluation of Samjunghwan, a traditional medicine, for neuroprotection against damage by amyloid-beta in rat cortical neurons / J Ethnopharmacol 130(3) : 625~630

[journal] 이상택 / 2008 / Experimental Study on the Antidepressant Effect of Sam-Jeong-Hwan / 동의신경정신과학회지 / 대한한방신경정신과학회 19(3) : 101~116

[journal] 이동준 / 2006 / Peroxynitrite Scavenging Activity of Samjunghwan / 대한한방내과학회지 / 대한한방내과학회 27(1) : 178~187

[journal] 송미영 / 2013 / Effect of Probiotics-Fermented Samjunghwan on Differentiation in 3T3-L1 Preadipocytes / 한국식품영양과학회지 / 한국식품영양과학회 42(1) : 1~7

[journal] 정해주 / 2006 / Effects of Samjunghwan on Obesity and Lipid Metabolism in High Fat Diet Rats / 대한한의학회지 / 대한한의학회 27(3) : 14~24

[journal] 송미영 / 2013 / Anti-Obesity Effects of Fermented Samjung-hwan in High Fat Diet Rats / 한방비만학회지 / 한방비만학회 13(1) : 17~23

[journal] 김용호 / 2009 / Survey on the Preference Formula for the Improvement of Oriental Herbal Medicine Insurance / 대한본초학회지 / 대한본초학회 24(4) : 17~23

[journal] Ainsworth EA / 2007 / Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin–Ciocalteu reagent / Nature Protoc 2(4) : 875~877

[journal] Prakash D / 2007 / Total phenol, antioxidant and free radical scavenging activities of some medicinal plants / Int J Food Sci Nutr 58(1) : 18~28

[journal] 전혜련 / 2012 / Antioxidant Activities and Quality Characteristics of Mulberry Concentrate, Freeze-dried Mulberry, and Pomace / 한국식품영양과학회지 / 한국식품영양과학회 41(10) : 1402~1408

[journal] Ancerewicz J / 1998 / Structure–property relationships of trimetazidine derivatives and model compounds as potential antioxidants / Free Rad Biol Med 25(1) : 113~120

[journal] Feuerstein GZ / 1993 / Cytokines, inflammation, and brain injury: role of tumor necrosis factor-alpha / Cerebrovasc Brain Metab Rev 6(4) : 341~360

[journal] Towler MC / 2007 / AMP-activated protein kinase in metabolic control and insulin signaling / Circ Res 100(3) : 328~341

[journal] Yin W / 2003 / Role of AMP-cerebrovascular kinase in cyclic AMP-dependent lipolysis In 3T3-L1 adipocytes / J Biol Chem 278(44) : 43074~43080

[journal] Rosen ED / 1999 / PPARγ is required for the differentiation of adipose tissue in vivo and in vitro / Mol Cell 4(4) : 611~617

[journal] 이지현 / 2014 / Peroxisome Proliferator-Activated Receptor (PPAR) α/γ Agonist / 대한내과학회지 / 대한내과학회 87(1) : 19~25

[journal] Tobert JA / 1987 / New developments in lipid-lowering therapy: the role of inhibitors of hydroxymethylglutaryl-coenzyme A reductase / Circulation 76(3) : 534~538

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