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The Effects of Astragali Radix Extracts on Mitochondrial Function in C2C12 Myotubes

  • Journal of Korean Medicine for Obesity Research
  • Abbr : J Korean Med Obes Res
  • 2014, 14(2), pp.55-62
  • Publisher : The Society of Korean Medicine for Obesity Research
  • Research Area : Medicine and Pharmacy > Korean Medicine
  • Published : December 30, 2014

송미영 1

1동국대학교

Candidate

ABSTRACT

Mitochondrial dysfunction is known to be involved in insulin resistance and obesity, researcheshave been increasing highly. Astragali Radix extract (ARE) or its main components have beenshown to perform comparably to insulin by significantly reducing blood glucose levels in animalmodels however, the influence on mitochondrial dysfunction are not well understood. Methods: ARE (0.2, 0.5 and 1.0 mg/ml) or metformin (2.5 mM) were treated in C2C12 after 6day-differentiation. The expressions of adenosine monophosphate (AMP)-activated proteinkinase (AMPK) and phosphorylation AMPK, peroxisome proliferators-activated receptror γcoactivator 1α (PGC1α), nuclear respiratory factors 1 (NRF1), mitochondrial transcription factor(Tfam) and myosin heavy chain were detected with western blotting or polymerase chain reactionanalysis. The morphological changes were also investigated. Results: ARE dose dependently increased phosphorylation of AMPK and respectively activatedmRNA expressions of PGC1α, NRF1 and Tfam which are mitochondrial biogenesis regulators. Furthermore, there were some morphologic differences of differentiated cells between AREtreatment and control. Conclusions: This study suggests that ARE has the potential to increase muscle mitochondrialfunction by activating AMPK and PGC1α.

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This paper was written with support from the National Research Foundation of Korea.