The Effects of Astragali Radix Extracts on Mitochondrial Function in C2C12 Myotubes (C2C12 골격근세포에서 황기의 미토콘드리아 조절 작용)

송미영

The Effects of Astragali Radix Extracts on Mitochondrial Function in C2C12 Myotubes

Journal of Korean Medicine for Obesity Research
Abbr : J Korean Med Obes Res
2014, 14(2), pp.55-62
Publisher : The Society of Korean Medicine for Obesity Research
Research Area : Medicine and Pharmacy > Korean Medicine
Published : December 30, 2014

송미영 ¹

¹동국대학교

Candidate

ABSTRACT

Mitochondrial dysfunction is known to be involved in insulin resistance and obesity, researcheshave been increasing highly. Astragali Radix extract (ARE) or its main components have beenshown to perform comparably to insulin by significantly reducing blood glucose levels in animalmodels however, the influence on mitochondrial dysfunction are not well understood. Methods: ARE (0.2, 0.5 and 1.0 mg/ml) or metformin (2.5 mM) were treated in C2C12 after 6day-differentiation. The expressions of adenosine monophosphate (AMP)-activated proteinkinase (AMPK) and phosphorylation AMPK, peroxisome proliferators-activated receptror γcoactivator 1α (PGC1α), nuclear respiratory factors 1 (NRF1), mitochondrial transcription factor(Tfam) and myosin heavy chain were detected with western blotting or polymerase chain reactionanalysis. The morphological changes were also investigated. Results: ARE dose dependently increased phosphorylation of AMPK and respectively activatedmRNA expressions of PGC1α, NRF1 and Tfam which are mitochondrial biogenesis regulators. Furthermore, there were some morphologic differences of differentiated cells between AREtreatment and control. Conclusions: This study suggests that ARE has the potential to increase muscle mitochondrialfunction by activating AMPK and PGC1α.

KEYWORDS

Astragali radix extract, Mitochondria, Myotube, AMP-activated protein kinases, Proliferators-activated receptror γ coactivator 1α

Citation status

* References for papers published after 2023 are currently being built.

[journal] Wallace DC / 2013 / A mitochondrial bioenergetic etiology of disease / J Clin Invest 123(4) : 1405~1412

[journal] Lee HK / 2010 / Mitochondrial dysfunction and metabolic syndrome−looking for environmental factors / Biochimica et Biophysica Acta 1800(3) : 282~289

[journal] Lowell BB / 2005 / Mitochondrial dysfunction and type 2diabetes / Science 307(5708) : 384~387

[journal] Kim JA / 2008 / Role of mitochondrial dysfunction in insulin resistance / Circ Res 102(4) : 401~414

[journal] 추효정 / 2006 / Type 2 Diabetes and Mitochondria / Endocrinology and Metabolism / 대한내분비학회 21(4) : 266~271

[journal] Suwa M / 2006 / Metformin increases the PGC-1alpha protein and oxidative enzyme activities possibly via AMPK phosphorylation in skeletal muscle in vivo / J Appl Physiol (1985) 101(6) : 1685~1692

[journal] Lagouge M / 2006 / Resveratrol improves mitochondrial function and protects against metabolic disease by activating SIRT1 and PGC-1alpha / Cell 127(6) : 1109~1122

[journal] 최철수 / 2009 / Potential Therapeutic Strategies for Fat Induced Insulin Resistance / Endocrinology and Metabolism / 대한내분비학회 24(2) : 65~74

[book] Association of professors of herbology / 2006 / Korean medicine / Yonglimsa : 579~580

[journal] Liu M / 2010 / Astragalus polysaccharide improves insulin sensitivity in KKAy mice:regulation of PKB/GLUT4 signaling in skeletal muscle / J Ethnopharmacol 127(1) : 32~37

[journal] Zou F / 2009 / Astragalus polysaccharides alleviates glucose toxicity and restores glucose homeostasis in diabetic states via activation of AMPK / Acta Pharmacol Sin 30(12) : 1607~1615

[journal] Shao BM / 2004 / A study on the immune receptors for polysaccharides from the roots of Astragalus membranaceus, a Chinese medicinal herb / Biochem Biophys Res Commun 320(4) : 1103~1111

[journal] Chen R / 2011 / Treatment with Astragalus membranaceus produces antioxidative effects and attenuates intestinal mucosa injury induced by intestinal ischemia-reperfusion in rats / Am J Chin Med 39(5) : 879~887

[journal] Li XT / 2012 / Mitochondrial protection and anti-aging activity of Astragalus polysaccharides and their potential mechanism / Int J Mol Sci 13(2) : 1747~1761

[journal] Cantó C / 2009 / PGC-1alpha, SIRT1 and AMPK, an energy sensing network that controls energy expenditure / Curr Opin Lipidol 20(2) : 98~105

[journal] Gleyzer N / 2005 / Control of mitochondrial transcription specificity factors (TFB1M and TFB2M) by nuclear respiratory factors (NRF-1 and NRF-2) and PGC-1 family coactivators / Mol Cell Biol 25(4) : 1354~1366

[journal] Rochard P / 2000 / Mitochondrial activity is involved in the regulation of myoblast differentiation through myogenin expression and activity of myogenic factors / J Biol Chem 275(4) : 2733~2744

[journal] Dedieu S / 2002 / Involvement of myogenic regulator factors during fusion in the cell line C2C12 / Int J Dev Biol 46(2) : 235~241

This paper was written with support from the National Research Foundation of Korea.

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Journal of Korean Medicine for Obesity Research 2023 KCI Impact Factor : 0.43