Screening of Herbal Medicines for Synergistic Effects of Metformin and Herbal Extracts Combination in RAW 264.7 Cells (RAW 264.7 세포에서 Metformin과 병행투여 시 상승효과를 나타내는 한약재의 선별 연구)

김형구; Jing-Hua Wang (WANG JINGHUA); 채희성; CHIN, YOUNG-WON (진영원); 최한석; Hojun Kim (김호준)

doi:10.15429/jkomor.2014.14.1.13

Screening of Herbal Medicines for Synergistic Effects of Metformin and Herbal Extracts Combination in RAW 264.7 Cells

Journal of Korean Medicine for Obesity Research
Abbr : J Korean Med Obes Res
2014, 14(1), pp.13-23
DOI : 10.15429/jkomor.2014.14.1.13
Publisher : The Society of Korean Medicine for Obesity Research
Research Area : Medicine and Pharmacy > Korean Medicine
Published : June 30, 2014

김형구 ¹, Jing-Hua Wang ², 채희성 ¹, CHIN, YOUNG-WON ¹, 최한석 ¹, Hojun Kim ¹

¹동국대학교
²동국대학교(경주캠퍼스) 한의학연구소

Candidate

ABSTRACT

Objectives: This study was performed to evaluate the effects of Metformin and Lonicerae Flos,Agrobacterium Rhizogenes, Coptidis Rhizoma, Atractylodis Rhizoma Alba, Houttuyniae Herbaextracs combinations on hypoglycemia in RAW 264.7 cells. Methods: Expressions of Sirt1, p-adenosine monophosphate-activated kinase (p-AMPK), AMPKalpha,peroxisome proliferator activated receptor (PPAR)-alpha, PPAR-gamma, X-box bindingprotein 1 (XBP-1), tumor necrosis factor (TNF)-alpha and interleukin (IL)-6 were analyzed by realtime polymerase chain reaction and Western blotting analysis. Results: The level of gene expression of Sirt1, p-AMPK, AMPK-alpha, PPAR-alpha and XBP-1 inrelation to that of beta-actin were increased or decreased significantly with the Metformin andLonicerae Flos, Agrobacterium Rhizogenes extracts combination groups. The level of geneexpression of TNF-alpha and IL-6 were increased significantly with the Metformin andHouttuyniae Herba, Coptidis Rhizoma extracts combination groups. Conclusions: Metformin and Lonicerae Flos, Agrobacterium Rhizogenes extracts combinationgroups showed synergistic hypoglycemic effects by increasing AMPK and PPAR gene expressionin RAW 264.7 cells

KEYWORDS

Metformin, Herbal medicines, Synergistic effects, Combination, Hypoglycemia

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Journal of Korean Medicine for Obesity Research 2023 KCI Impact Factor : 0.43

Screening of Herbal Medicines for Synergistic Effects of Metformin and Herbal Extracts Combination in RAW 264.7 Cells

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* References for papers published after 2023 are currently being built.

Journal of Korean Medicine for Obesity Research 2023 KCI Impact Factor : 0.43

Screening of Herbal Medicines for Synergistic Effects of Metformin and Herbal Extracts Combination in RAW 264.7 Cells

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