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Screening of Herbal Medicines for Synergistic Effects of Metformin and Herbal Extracts Combination in RAW 264.7 Cells

  • Journal of Korean Medicine for Obesity Research
  • Abbr : J Korean Med Obes Res
  • 2014, 14(1), pp.13-23
  • DOI : 10.15429/jkomor.2014.14.1.13
  • Publisher : The Society of Korean Medicine for Obesity Research
  • Research Area : Medicine and Pharmacy > Korean Medicine
  • Published : June 30, 2014

김형구 1 Jing-Hua Wang 2 채희성 1 CHIN, YOUNG-WON 1 최한석 1 Hojun Kim 1

1동국대학교
2동국대학교(경주캠퍼스) 한의학연구소

Candidate

ABSTRACT

Objectives: This study was performed to evaluate the effects of Metformin and Lonicerae Flos,Agrobacterium Rhizogenes, Coptidis Rhizoma, Atractylodis Rhizoma Alba, Houttuyniae Herbaextracs combinations on hypoglycemia in RAW 264.7 cells. Methods: Expressions of Sirt1, p-adenosine monophosphate-activated kinase (p-AMPK), AMPKalpha,peroxisome proliferator activated receptor (PPAR)-alpha, PPAR-gamma, X-box bindingprotein 1 (XBP-1), tumor necrosis factor (TNF)-alpha and interleukin (IL)-6 were analyzed by realtime polymerase chain reaction and Western blotting analysis. Results: The level of gene expression of Sirt1, p-AMPK, AMPK-alpha, PPAR-alpha and XBP-1 inrelation to that of beta-actin were increased or decreased significantly with the Metformin andLonicerae Flos, Agrobacterium Rhizogenes extracts combination groups. The level of geneexpression of TNF-alpha and IL-6 were increased significantly with the Metformin andHouttuyniae Herba, Coptidis Rhizoma extracts combination groups. Conclusions: Metformin and Lonicerae Flos, Agrobacterium Rhizogenes extracts combinationgroups showed synergistic hypoglycemic effects by increasing AMPK and PPAR gene expressionin RAW 264.7 cells

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