Effects of Fermented Lotus Extracts on the Differentiation in 3T3-L1 Preadipocytes (3T3-L1 전지방세포에서 연잎-연근 혼합 발효물의 지방세포 분화 억제 효과)

이신지; Shambhunath Bose (보세); 이수진; 정지은; Byung-soo Koo (구병수); Kim, Dong il (김동일); Hojun Kim (김호준)

Effects of Fermented Lotus Extracts on the Differentiation in 3T3-L1 Preadipocytes

Journal of Korean Medicine for Obesity Research
Abbr : J Korean Med Obes Res
2013, 2(2), pp.74-83
Publisher : The Society of Korean Medicine for Obesity Research
Research Area : Medicine and Pharmacy > Korean Medicine

이신지 ¹, Shambhunath Bose ², 이수진 ³, 정지은 ¹, Byung-soo Koo ⁴, Kim, Dong il ⁵, Hojun Kim ¹

¹동국대학교 한의과대학 한방재활의학과교실
²동국대학교
³동국대학교 한의과대학 한방재활의학과 교실
⁴동국대학교 한의과대학 한방신경정신과학교실
⁵동국대학교 한의과대학 한방부인과학교실

ABSTRACT

Objectives: This study was performed to evaluate the effects of fermented lotus extracts on theinhibition of differentiation in 3T3-L1 preadipocytes. Methods: Extracts of lotus leaf and lotus root were fermented using 4 different probioticsseparately, including Lactobacillus plantarum, Lactobacillus rhamnosus, Bifidobacterium breve,and Bifidobacterium longum. Inhibition of preadipocyte differentiation was examined by Oil red Odye staining. Expressions of adipogenic transcription factors including CCAAT/enhancer bindingproteins (C/EBPα) and peroxisome proliferators-activated receptor γ (PPARγ) were analyzed byreal time polymerase chain reaction and Western blotting analysis. Results: Fermented lotus extracts inhibited adipogenic transcription factors by inhibitingpreadipocytes differentiation. All of the groups fermented by 4 kinds of probiotics showedreduction in Oil Red O dye staining. Bifidobacterium breve showed the most effective inhibitionof C/EBPα. Bifidobacterium breve and Bifidobacterium longum showed the best downregulationof PPARγ expressions compared with the control and the unfermented lotus group. Conclusions: Fermented lotus extracts showed significant effects on inhibition of preadipocytedifferentiation in 3T3-L1 preadipocytes showing correlation with insulin sensitivity and lipidmetabolism related with obesity.

KEYWORDS

3T3-L1 preadipocyte, Lotus, Probiotics, Fermentation, Obesity, Insulin resistance

Citation status

* References for papers published after 2023 are currently being built.

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