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Effects of Fermented Lotus Extracts on the Differentiation in 3T3-L1 Preadipocytes

  • Journal of Korean Medicine for Obesity Research
  • Abbr : J Korean Med Obes Res
  • 2013, 2(2), pp.74-83
  • Publisher : The Society of Korean Medicine for Obesity Research
  • Research Area : Medicine and Pharmacy > Korean Medicine

이신지 1 Shambhunath Bose 2 이수진 3 정지은 1 Byung-soo Koo 4 Kim, Dong il ORD ID 5 Hojun Kim 1

1동국대학교 한의과대학 한방재활의학과교실
2동국대학교
3동국대학교 한의과대학 한방재활의학과 교실
4동국대학교 한의과대학 한방신경정신과학교실
5동국대학교 한의과대학 한방부인과학교실

ABSTRACT

Objectives: This study was performed to evaluate the effects of fermented lotus extracts on theinhibition of differentiation in 3T3-L1 preadipocytes. Methods: Extracts of lotus leaf and lotus root were fermented using 4 different probioticsseparately, including Lactobacillus plantarum, Lactobacillus rhamnosus, Bifidobacterium breve,and Bifidobacterium longum. Inhibition of preadipocyte differentiation was examined by Oil red Odye staining. Expressions of adipogenic transcription factors including CCAAT/enhancer bindingproteins (C/EBPα) and peroxisome proliferators-activated receptor γ (PPARγ) were analyzed byreal time polymerase chain reaction and Western blotting analysis. Results: Fermented lotus extracts inhibited adipogenic transcription factors by inhibitingpreadipocytes differentiation. All of the groups fermented by 4 kinds of probiotics showedreduction in Oil Red O dye staining. Bifidobacterium breve showed the most effective inhibitionof C/EBPα. Bifidobacterium breve and Bifidobacterium longum showed the best downregulationof PPARγ expressions compared with the control and the unfermented lotus group. Conclusions: Fermented lotus extracts showed significant effects on inhibition of preadipocytedifferentiation in 3T3-L1 preadipocytes showing correlation with insulin sensitivity and lipidmetabolism related with obesity.

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