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The Anti-Inflammatory Effect of Pulsatilla koreana Methanol Extract in Lipopolysaccharid-Exposed RAW 264.7 Cells

  • Journal of Korean Medicine for Obesity Research
  • Abbr : J Korean Med Obes Res
  • 2017, 17(1), pp.1-9
  • Publisher : The Society of Korean Medicine for Obesity Research
  • Research Area : Medicine and Pharmacy > Korean Medicine
  • Published : June 30, 2017

이귀선 1 김두희 1 박중현 2 최희승 1 허성규 3 Cha Yun Yeop 4

1상지대학교 한의과대학 한방재활의학과교실
2상지대학교 한의과대학 한방재활의학교실
3상지대학교 한의과대학 한방재활의학과학교실
4상지대학교

Accredited

ABSTRACT

Objectives: This study was performed to identify the anti-inflammatory effect of Pulsatilla koreana (PK) methanol extract on lipopolysaccharide (LPS) induced inflammatory. Methods: There were five groups. They were control group, LPS-exposed PK methanol extract group (0 mg/ml, 10 mg/ml, 30 mg/ml, 100 mg/ml). To measure out cytotoxicity of PK, we performed the MTT assay. To evaluate the anti-inflammatory effect of PK, we examined the inflammatory mediators, such as nitric oxide (NO), prostaglandin E2 (PGE2), and pro- inflammatory cytokines (interleukin [IL]-1b, IL-6, tumor necrosis factor-a [TNF-a], IL-10). Results: 1. The extract of PK (∼100 mg/ml) itself did not have any cytotoxic effect in RAW 264.7 cells. 2. The concentration of plasma NO and PGE2 in PK methanol extract group showed a lower values than those of control group. 3. The concentration of plasma IL-1b, plasma IL-6, and plasma TNF-a in PK methanol extract group showed a lower values than those of control group. 4. The concentration of Plasma IL-10 in PK methanol extract groups showed higher than control group; however, these values showed no significantly different. Conclusions: According to this study, the extract of PK could be used as a protective agent against inflammation.

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