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@article{ART002364531},
author={KIM, BYUNG JOO},
title={Effects of Dangkwisoo-San, Ginger and Curcumin on Transient Receptor Potential Melastatin 7 Channels},
journal={Journal of Korean Medicine for Obesity Research},
issn={1976-9334},
year={2018},
volume={18},
number={1},
pages={10-18},
doi={10.15429/jkomor.2018.18.1.10}

TY - JOUR
AU - KIM, BYUNG JOO
TI - Effects of Dangkwisoo-San, Ginger and Curcumin on Transient Receptor Potential Melastatin 7 Channels
JO - Journal of Korean Medicine for Obesity Research
PY - 2018
VL - 18
IS - 1
PB - The Society of Korean Medicine for Obesity Research
SP - 10
EP - 18
SN - 1976-9334
AB - Objectives: Metabolic syndrome is correlated with increased cardiovascular risk and characterized by several factors, including visceral obesity, hypertension, insulin resistance, and dyslipidemia. Several members of a large family of nonselective cation entry channels, e.g., transient receptor potential (TRP) melastatin 7 (TRPM7) channels have been associated with the development of cardiovascular diseases. The purpose of this study was to investigate the effects of Dangkwisoo-san, ginger and curcumin on TRPM7 channel. Methods: Human embryonic kidney (HEK) 293 cells stably transfected with the TRPM7 expression vectors were maintained in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin/streptomycin, 5 µg/mL blasticidin, and 0.4 mg/mL zeocin in a humidified 20% O2/10% CO2 atmosphere at 37℃. Whole-cell patch clamp recordings were obtained using an Axopatch 700B amplifier and pClamp v.10.4 software, and signals were digitalized at 5 kHz using Digidata 1422A. Results: Dangkwisoo-san extract (100, 200, 300, 400, and 500 µg/mL) inhibited the outward and inward TRPM7 whole-cell currents at dose dependent manner and the half maximal inhibitory concentration (IC)50 of Dangkwisoo-san was 218.3 µg/mL. Also, ginger extract (100, 200, 300, 400, and 500 µg/mL) inhibited the outward and inward of TRPM7 whole-cell currents in a dose dependent manner and the IC50 of ginger was 877.2 µg/mL. However, curcumin had no effects on TRPM7 whole-cell currents. Conclusions: These results suggest that both Dangkwisoo-san and ginger have good roles to inhibit the TRPM7 channel, suggesting that Dangkwisoo-san and ginger are considered one of the candidate agents for the treatment of metabolic syndrome such as cardiovascular disease.
KW - Metabolic syndrome;Ginger;Curcumin
DO - 10.15429/jkomor.2018.18.1.10
ER -

KIM, BYUNG JOO. (2018). Effects of Dangkwisoo-San, Ginger and Curcumin on Transient Receptor Potential Melastatin 7 Channels. Journal of Korean Medicine for Obesity Research, 18(1), 10-18.

KIM, BYUNG JOO. 2018, "Effects of Dangkwisoo-San, Ginger and Curcumin on Transient Receptor Potential Melastatin 7 Channels", Journal of Korean Medicine for Obesity Research, vol.18, no.1 pp.10-18. Available from: doi:10.15429/jkomor.2018.18.1.10

KIM, BYUNG JOO "Effects of Dangkwisoo-San, Ginger and Curcumin on Transient Receptor Potential Melastatin 7 Channels" Journal of Korean Medicine for Obesity Research 18.1 pp.10-18 (2018) : 10.

KIM, BYUNG JOO. Effects of Dangkwisoo-San, Ginger and Curcumin on Transient Receptor Potential Melastatin 7 Channels. 2018; 18(1), 10-18. Available from: doi:10.15429/jkomor.2018.18.1.10

KIM, BYUNG JOO. "Effects of Dangkwisoo-San, Ginger and Curcumin on Transient Receptor Potential Melastatin 7 Channels" Journal of Korean Medicine for Obesity Research 18, no.1 (2018) : 10-18.doi: 10.15429/jkomor.2018.18.1.10

KIM, BYUNG JOO. Effects of Dangkwisoo-San, Ginger and Curcumin on Transient Receptor Potential Melastatin 7 Channels. Journal of Korean Medicine for Obesity Research, 18(1), 10-18. doi: 10.15429/jkomor.2018.18.1.10

KIM, BYUNG JOO. Effects of Dangkwisoo-San, Ginger and Curcumin on Transient Receptor Potential Melastatin 7 Channels. Journal of Korean Medicine for Obesity Research. 2018; 18(1) 10-18. doi: 10.15429/jkomor.2018.18.1.10

KIM, BYUNG JOO. Effects of Dangkwisoo-San, Ginger and Curcumin on Transient Receptor Potential Melastatin 7 Channels. 2018; 18(1), 10-18. Available from: doi:10.15429/jkomor.2018.18.1.10

KIM, BYUNG JOO. "Effects of Dangkwisoo-San, Ginger and Curcumin on Transient Receptor Potential Melastatin 7 Channels" Journal of Korean Medicine for Obesity Research 18, no.1 (2018) : 10-18.doi: 10.15429/jkomor.2018.18.1.10