Membrane Free Stem Cell Extract from Adipose Tissue Enhances Glucose Uptake in 3T3-L1 Cells (무막줄기세포추출물의 3T3-L1 세포에서 포도당 흡수 촉진 효과)

Ji Hyun Kim (김지현); Min Jeong Kim (김민정); Hye Sook Park (박혜숙); Young Sil Kim (김영실); Eun Ju  Cho (조은주)

doi:10.15429/jkomor.2019.19.2.89

Membrane Free Stem Cell Extract from Adipose Tissue Enhances Glucose Uptake in 3T3-L1 Cells

Journal of Korean Medicine for Obesity Research
Abbr : J Korean Med Obes Res
2019, 19(2), pp.89-96
DOI : 10.15429/jkomor.2019.19.2.89
Publisher : The Society of Korean Medicine for Obesity Research
Research Area : Medicine and Pharmacy > Korean Medicine
Received : October 1, 2019
Accepted : October 28, 2019
Published : December 30, 2019

Ji Hyun Kim ¹, Min Jeong Kim ¹, Hye Sook Park ², Young Sil Kim ², Eun Ju Cho ¹

¹부산대학교 식품영양학과
²㈜티스템

Accredited

ABSTRACT

Objectives: We investigated whether membrane free stem cell extract from adipose tissue (MFSCE) has anti-diabetic effect. Methods: To determine glucose uptake effect of MFSCE, we carried out glucose uptake assay in 3T3-L1 adipocytes. The regulatory mechanisms of MFSCE on glucose uptake were examined by Western blot analysis. Results: When MFSCE was treated to adipocytes at the concentration of 0.5, 1, 2.5, and 5 μg/mL, 2-deoxyglucose-6-phosphate uptake was elevated approximately 1.8-fold compared to cells not treated with MFSCE. It indicated that MFSCE enhances glucose uptake in 3T3-L1 adipocytes. In addition, MFSCE reduced phosphorylation of insulin receptor substrate-1 at serine 307 and induced Akt and glucose transporter 4 protein expressions that were related to insulin signaling. Furthermore, MFSCE regulated adenosine monophosphate-activated protein kinase (AMPK) pathway by increases of increase phosphorylation of AMPK and acetyl-CoA carboxylase that were related to AMPK pathway. Conclusions: These results indicated that MFSCE promotes glucose uptake via modulation of insulin signaling and AMPK pathway. Therefore, MFSCE could be a promising agent for treatment of diabetes mellitus.

KEYWORDS

AMP-activated protein kinases, Diabetes mellitus, Membrane free stem cell extract, 3T3-L1 cells

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Journal of Korean Medicine for Obesity Research 2023 KCI Impact Factor : 0.43

Membrane Free Stem Cell Extract from Adipose Tissue Enhances Glucose Uptake in 3T3-L1 Cells

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* References for papers published after 2023 are currently being built.

Journal of Korean Medicine for Obesity Research 2023 KCI Impact Factor : 0.43

Membrane Free Stem Cell Extract from Adipose Tissue Enhances Glucose Uptake in 3T3-L1 Cells

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