@article{ART002538536},
author={Ji Hyun Kim and Min Jeong Kim and Hye Sook Park and Young Sil Kim and Eun Ju Cho},
title={Membrane Free Stem Cell Extract from Adipose Tissue Enhances Glucose Uptake in 3T3-L1 Cells},
journal={Journal of Korean Medicine for Obesity Research},
issn={1976-9334},
year={2019},
volume={19},
number={2},
pages={89-96},
doi={10.15429/jkomor.2019.19.2.89}
TY - JOUR
AU - Ji Hyun Kim
AU - Min Jeong Kim
AU - Hye Sook Park
AU - Young Sil Kim
AU - Eun Ju Cho
TI - Membrane Free Stem Cell Extract from Adipose Tissue Enhances Glucose Uptake in 3T3-L1 Cells
JO - Journal of Korean Medicine for Obesity Research
PY - 2019
VL - 19
IS - 2
PB - The Society of Korean Medicine for Obesity Research
SP - 89
EP - 96
SN - 1976-9334
AB - Objectives: We investigated whether membrane free stem cell extract from adipose tissue (MFSCE) has anti-diabetic effect.
Methods: To determine glucose uptake effect of MFSCE, we carried out glucose uptake assay in 3T3-L1 adipocytes. The regulatory mechanisms of MFSCE on glucose uptake were examined by Western blot analysis.
Results: When MFSCE was treated to adipocytes at the concentration of 0.5, 1, 2.5, and 5 μg/mL, 2-deoxyglucose-6-phosphate uptake was elevated approximately 1.8-fold compared to cells not treated with MFSCE. It indicated that MFSCE enhances glucose uptake in 3T3-L1 adipocytes. In addition, MFSCE reduced phosphorylation of insulin receptor substrate-1 at serine 307 and induced Akt and glucose transporter 4 protein expressions that were related to insulin signaling. Furthermore, MFSCE regulated adenosine monophosphate-activated protein kinase (AMPK) pathway by increases of increase phosphorylation of AMPK and acetyl-CoA carboxylase that were related to AMPK pathway.
Conclusions: These results indicated that MFSCE promotes glucose uptake via modulation of insulin signaling and AMPK pathway. Therefore, MFSCE could be a promising agent for treatment of diabetes mellitus.
KW - AMP-activated protein kinases;Diabetes mellitus;Membrane free stem cell extract;3T3-L1 cells
DO - 10.15429/jkomor.2019.19.2.89
ER -
Ji Hyun Kim, Min Jeong Kim, Hye Sook Park, Young Sil Kim and Eun Ju Cho. (2019). Membrane Free Stem Cell Extract from Adipose Tissue Enhances Glucose Uptake in 3T3-L1 Cells. Journal of Korean Medicine for Obesity Research, 19(2), 89-96.
Ji Hyun Kim, Min Jeong Kim, Hye Sook Park, Young Sil Kim and Eun Ju Cho. 2019, "Membrane Free Stem Cell Extract from Adipose Tissue Enhances Glucose Uptake in 3T3-L1 Cells", Journal of Korean Medicine for Obesity Research, vol.19, no.2 pp.89-96. Available from: doi:10.15429/jkomor.2019.19.2.89
Ji Hyun Kim, Min Jeong Kim, Hye Sook Park, Young Sil Kim, Eun Ju Cho "Membrane Free Stem Cell Extract from Adipose Tissue Enhances Glucose Uptake in 3T3-L1 Cells" Journal of Korean Medicine for Obesity Research 19.2 pp.89-96 (2019) : 89.
Ji Hyun Kim, Min Jeong Kim, Hye Sook Park, Young Sil Kim, Eun Ju Cho. Membrane Free Stem Cell Extract from Adipose Tissue Enhances Glucose Uptake in 3T3-L1 Cells. 2019; 19(2), 89-96. Available from: doi:10.15429/jkomor.2019.19.2.89
Ji Hyun Kim, Min Jeong Kim, Hye Sook Park, Young Sil Kim and Eun Ju Cho. "Membrane Free Stem Cell Extract from Adipose Tissue Enhances Glucose Uptake in 3T3-L1 Cells" Journal of Korean Medicine for Obesity Research 19, no.2 (2019) : 89-96.doi: 10.15429/jkomor.2019.19.2.89
Ji Hyun Kim; Min Jeong Kim; Hye Sook Park; Young Sil Kim; Eun Ju Cho. Membrane Free Stem Cell Extract from Adipose Tissue Enhances Glucose Uptake in 3T3-L1 Cells. Journal of Korean Medicine for Obesity Research, 19(2), 89-96. doi: 10.15429/jkomor.2019.19.2.89
Ji Hyun Kim; Min Jeong Kim; Hye Sook Park; Young Sil Kim; Eun Ju Cho. Membrane Free Stem Cell Extract from Adipose Tissue Enhances Glucose Uptake in 3T3-L1 Cells. Journal of Korean Medicine for Obesity Research. 2019; 19(2) 89-96. doi: 10.15429/jkomor.2019.19.2.89
Ji Hyun Kim, Min Jeong Kim, Hye Sook Park, Young Sil Kim, Eun Ju Cho. Membrane Free Stem Cell Extract from Adipose Tissue Enhances Glucose Uptake in 3T3-L1 Cells. 2019; 19(2), 89-96. Available from: doi:10.15429/jkomor.2019.19.2.89
Ji Hyun Kim, Min Jeong Kim, Hye Sook Park, Young Sil Kim and Eun Ju Cho. "Membrane Free Stem Cell Extract from Adipose Tissue Enhances Glucose Uptake in 3T3-L1 Cells" Journal of Korean Medicine for Obesity Research 19, no.2 (2019) : 89-96.doi: 10.15429/jkomor.2019.19.2.89