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Airway Microbiota in Stroke Patients with Tracheostomy: A Pilot Study

  • Journal of Korean Medicine for Obesity Research
  • Abbr : J Korean Med Obes Res
  • 2019, 19(2), pp.97-105
  • DOI : 10.15429/jkomor.2019.19.2.97
  • Publisher : The Society of Korean Medicine for Obesity Research
  • Research Area : Medicine and Pharmacy > Korean Medicine
  • Received : October 31, 2019
  • Accepted : December 2, 2019
  • Published : December 30, 2019

Eunhak Seong 1 Yura Choi 1 Sookyoung Lim 1 Myeong-Jong Lee 1 Youngdo Nam 2 Eunji Song 2 Hojun Kim 1

1동국대학교 한의과대학 한방재활의학과교실
2한국식품연구원 장내미생물연구단

Accredited

ABSTRACT

Objectives: We investigated differences between the tracheostomized and the non-tracheostomized stroke patients through microbiological analysis for the purpose of preliminary explorations of full-scale clinical research in the future. Methods: We collected tracheal aspirates samples from 5 stroke patients with tracheostomy and expectorated sputum samples from 5 stroke patients without tracheostomy. Genomic DNA from sputum samples was isolated using QIAamp DNA mini kit. The sequences were processed using Quantitative Insights into Microbial Ecology 1.9.0. Alpha-diversity was calculated using the Chao1 estimator. Beta-diversity was analyzed by UniFrac-based principal coordinates analysis (PCoA). To confirm taxa with different abundance among the groups, linear discriminant analysis effect size analysis was performed. Results: Although alpha-diversity value of the tracheostomized group was higher than that of the non-tracheostomized group, there was no statistically significant difference. In PCoA, clear separation was seen between clusters of the tracheostomized group and that of the non-tracheostomized group. In both groups, Bacteroidetes, Proteobacteria, Fusobacteria, Firmicutes, Actinobacteria were identified as dominant in phylum level. In particular, relative richness of Proteobacteria was found to be 31% more in the tracheotomized group (36.6%) than the non-tracheostomized group (5.6%)(P<0.05). In genus level, Neisseria (24%), Prevotella (17%), Streptococcus (13%), Fusobacteria (11%), Porphyromonas (7%) were identified as dominant in the tracheostomized group. In the non-tracheostomized group, Prevotella (38%), Veillonella (20%), Neisseria (9%) were genera that found to be dominant. Conclusions: It is meaningful in that the tracheostomized group has been identified a higher rate of microbiotas known as pathogenic in respiratory diseases compared to the non-tracheostomized group, confirming the possibility that the risk of opportunity infection may be higher.

Citation status

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This paper was written with support from the National Research Foundation of Korea.