Airway Microbiota in Stroke Patients with Tracheostomy: A Pilot Study (기관절개술을 시행한 뇌졸중 환자들에서의 기도미생물 탐색 연구)

Eunhak Seong (성은학); Yura Choi (최유라); Sookyoung Lim (임수경); Myeong-Jong Lee (이명종); Youngdo Nam (남영도); Eunji Song (송은지); Hojun Kim (김호준)

doi:10.15429/jkomor.2019.19.2.97

Airway Microbiota in Stroke Patients with Tracheostomy: A Pilot Study

Journal of Korean Medicine for Obesity Research
Abbr : J Korean Med Obes Res
2019, 19(2), pp.97-105
DOI : 10.15429/jkomor.2019.19.2.97
Publisher : The Society of Korean Medicine for Obesity Research
Research Area : Medicine and Pharmacy > Korean Medicine
Received : October 31, 2019
Accepted : December 2, 2019
Published : December 30, 2019

Eunhak Seong ¹, Yura Choi ¹, Sookyoung Lim ¹, Myeong-Jong Lee ¹, Youngdo Nam ², Eunji Song ², Hojun Kim ¹

¹동국대학교 한의과대학 한방재활의학과교실
²한국식품연구원 장내미생물연구단

Accredited

ABSTRACT

Objectives: We investigated differences between the tracheostomized and the non-tracheostomized stroke patients through microbiological analysis for the purpose of preliminary explorations of full-scale clinical research in the future. Methods: We collected tracheal aspirates samples from 5 stroke patients with tracheostomy and expectorated sputum samples from 5 stroke patients without tracheostomy. Genomic DNA from sputum samples was isolated using QIAamp DNA mini kit. The sequences were processed using Quantitative Insights into Microbial Ecology 1.9.0. Alpha-diversity was calculated using the Chao1 estimator. Beta-diversity was analyzed by UniFrac-based principal coordinates analysis (PCoA). To confirm taxa with different abundance among the groups, linear discriminant analysis effect size analysis was performed. Results: Although alpha-diversity value of the tracheostomized group was higher than that of the non-tracheostomized group, there was no statistically significant difference. In PCoA, clear separation was seen between clusters of the tracheostomized group and that of the non-tracheostomized group. In both groups, Bacteroidetes, Proteobacteria, Fusobacteria, Firmicutes, Actinobacteria were identified as dominant in phylum level. In particular, relative richness of Proteobacteria was found to be 31% more in the tracheotomized group (36.6%) than the non-tracheostomized group (5.6%)(P<0.05). In genus level, Neisseria (24%), Prevotella (17%), Streptococcus (13%), Fusobacteria (11%), Porphyromonas (7%) were identified as dominant in the tracheostomized group. In the non-tracheostomized group, Prevotella (38%), Veillonella (20%), Neisseria (9%) were genera that found to be dominant. Conclusions: It is meaningful in that the tracheostomized group has been identified a higher rate of microbiotas known as pathogenic in respiratory diseases compared to the non-tracheostomized group, confirming the possibility that the risk of opportunity infection may be higher.

KEYWORDS

Stroke, Tracheostomy, Microbiota

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[journal] Moffatt MF / 2017 / The lung microbiome in health and disease / Clinical Medicine 17(6) : 525~529

[journal] Hilty M / 2010 / Disordered microbial communities in asthmatic airways / PloS One 5(1) : e8578~

[journal] Man WH / 2017 / The microbiota of the respiratory tract: gatekeeper to respiratory health / Nature Reviews Microbiology 15(5) : 259~270

[journal] Bösel J / 2014 / Tracheostomy in stroke patients / Curr Treat Options Neurol 16(1) : 274~

[journal] McGrath B / 2016 / Above cuff vocalisation: A novel technique for communication in the ventilator-dependent tracheostomy patient / J Intensive Care Soc 17(1) : 19~26

[journal] Pérez-Losada M / 2018 / Tracheal microbiota in patients with a tracheostomy before, during and after an acute respiratory infection / Pediatr Infect Dis J 37(11) : e269~e271

[journal] Pérez-Losada M / 2017 / The temporal dynamics of the tracheal microbiome in tracheostomised patients with and without lower respiratory infections / PLoS One 12(8) : e0182520~

[journal] Shenoy MD / 2018 / Diagnostic accuracy of bronchoalveolar lavage in lung lesions / APALM 5(1) : A57~A59

[journal] Schnabel RM / 2015 / Clinical course and complications following diagnostic bronchoalveolar lavage in critically ill mechanically ventilated patients / BMC Pulm Med 15(1) : 107~

[book] Midulla F / 2010 / Paediatric bronchoscopy. Vol. 38 / Karger : 30~41

[journal] Huang YJ / 2011 / The emerging relationship between the airway microbiota and chronic respiratory disease:clinical implications / Expert Rev Respir Med 5(6) : 809~821

[journal] Biesbroek G / 2014 / Early respiratory microbiota composition determines bacterial succession patterns and respiratory health in children. American journal of respiratory and critical care medicine / Am J Respir Crit Care Med 190(11) : 1283~1292

[journal] Cameron JL / 1973 / Aspiration in patients with tracheostomies / Plast Reconstr Surg 136(1) : 68~70

[journal] Epstein SK / 2005 / Late complications of tracheostomy / Respir Care 50(4) : 542~549

[journal] Rinninella E / 2019 / What is the healthy gut microbiota composition? a changing ecosystem across age, environment, diet, and diseases / Microorganisms 7(1) : 14~

[journal] Huang YJ / 2011 / Airway microbiota and bronchial hyperresponsiveness in patients with suboptimally controlled asthma / J Allergy Clin Immunol 127(2) : 372~381

[journal] Zemanick ET / 2017 / Airway microbiota across age and disease spectrum in cystic fibrosis / Eur Respir J 50(5) : 1700832~

[journal] Mika M / 2018 / Microbial and host immune factors as drivers of COPD / ERJ Open Res 4(3)

[book] Hudcova J / 2013 / Hospital-acquired pneumonia / Future Medicine : 49~66

[journal] Hunter JD / 2012 / Ventilator associated pneumonia / BMJ 344 : e3325~

[journal] Pulvirenti G / 2019 / Lower airway microbiota / Front Pediatr 7 : 393~

[journal] Bittar F / 2010 / Detection and accurate identification of new or emerging bacteria in cystic fibrosis patients / Clin Microbiol Infect 16(7) : 809~820

[journal] Morris A / 2013 / Comparison of the respiratory microbiome in healthy nonsmokers and smokers / Am J Respir Crit Care Med 187(10) : 1067~1075

[journal] Larsen JM / 2012 / Divergent pro-inflammatory profile of human dendritic cells in response to commensal and pathogenic bacteria associated with the airway microbiota / PLoS One 7(2) : e31976~

[journal] Mathieu E / 2018 / Paradigms of lung microbiota functions in health and disease, particularly, in asthma / Front Physiol 9 : 1168~

This paper was written with support from the National Research Foundation of Korea.

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Journal of Korean Medicine for Obesity Research 2023 KCI Impact Factor : 0.43

Airway Microbiota in Stroke Patients with Tracheostomy: A Pilot Study

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* References for papers published after 2023 are currently being built.

Journal of Korean Medicine for Obesity Research 2023 KCI Impact Factor : 0.43

Airway Microbiota in Stroke Patients with Tracheostomy: A Pilot Study

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