@article{ART002598669},
author={Jang,Byeong-Churl},
title={Inhibitory Effect of Dihydroartemisinin, An Active Ingredient of Artemisia annua, on Lipid Accumulation in Differentiating 3T3-L1 Preadipocytes},
journal={Journal of Korean Medicine for Obesity Research},
issn={1976-9334},
year={2020},
volume={20},
number={1},
pages={1-9},
doi={10.15429/jkomor.2020.20.1.1}
TY - JOUR
AU - Jang,Byeong-Churl
TI - Inhibitory Effect of Dihydroartemisinin, An Active Ingredient of Artemisia annua, on Lipid Accumulation in Differentiating 3T3-L1 Preadipocytes
JO - Journal of Korean Medicine for Obesity Research
PY - 2020
VL - 20
IS - 1
PB - The Society of Korean Medicine for Obesity Research
SP - 1
EP - 9
SN - 1976-9334
AB - Objectives: Artemisinin and its derivatives extracted from Artemisia annua, a Chinese herbal medicine, have variable biological effects due to structural differences. Up to date, the anti-obesity effect of dihydroartemisinin (DHA), a derivative of artemisinin, is unknown. The purpose of this study was to investigate the anti-adipogenic and lipolytic effects of DHA on 3T3-L1 preadipocytes.
Methods: Oil Red O staining and AdipoRed assay were used to measure lipid accumulation and triglyceride (TG) content in 3T3-L1 cells, respectively. Cell count analysis was used to determine the cytotoxicity of 3T3-L1 cells. Western blot and real-time reverse transcription polymerase chain reaction analyses were used to analyze the expression of protein and mRNA in 3T3-L1 cells, respectively.
Results: DHA at 5 μM markedly inhibited lipid accumulation and reduced TG content in differentiating 3T3-L1 cells with no cytotoxicity. Furthermore, DHA at 5 μM inhibited the expression of CCAAT/enhancer-binding protein-α (C/EBP-α), peroxisome proliferator-activated receptor-γ (PPAR-γ), fatty acid synthase (FAS), and perilipin A as well as the phosphorylation of signal transducer and activator of transcription-3 (STAT-3) in differentiating 3T3-L1 cells. Moreover, while DHA at 5 μM had no effect on the mRNA expression of adiponectin, it strongly suppressed that of leptin in differentiating 3T3-L1 cells. However, DHA at 5 μM had no lipolytic effect on differentiated 3T3-L1 cells, as assessed by no enhancement of glycerol release.
Conclusions: These results demonstrate that DHA at 5 μM has a strong anti-adipogenic effect on differentiating 3T3-L1 cells through the reduced expression and phosphorylation of C/EBP-α, PPAR-γ, FAS, perilipin A, and STAT-3.
KW - Dihydroartemisinin;C/EBP-α;PPAR-γ;STAT-3;Perilipin A;3T3-L1
DO - 10.15429/jkomor.2020.20.1.1
ER -
Jang,Byeong-Churl. (2020). Inhibitory Effect of Dihydroartemisinin, An Active Ingredient of Artemisia annua, on Lipid Accumulation in Differentiating 3T3-L1 Preadipocytes. Journal of Korean Medicine for Obesity Research, 20(1), 1-9.
Jang,Byeong-Churl. 2020, "Inhibitory Effect of Dihydroartemisinin, An Active Ingredient of Artemisia annua, on Lipid Accumulation in Differentiating 3T3-L1 Preadipocytes", Journal of Korean Medicine for Obesity Research, vol.20, no.1 pp.1-9. Available from: doi:10.15429/jkomor.2020.20.1.1
Jang,Byeong-Churl "Inhibitory Effect of Dihydroartemisinin, An Active Ingredient of Artemisia annua, on Lipid Accumulation in Differentiating 3T3-L1 Preadipocytes" Journal of Korean Medicine for Obesity Research 20.1 pp.1-9 (2020) : 1.
Jang,Byeong-Churl. Inhibitory Effect of Dihydroartemisinin, An Active Ingredient of Artemisia annua, on Lipid Accumulation in Differentiating 3T3-L1 Preadipocytes. 2020; 20(1), 1-9. Available from: doi:10.15429/jkomor.2020.20.1.1
Jang,Byeong-Churl. "Inhibitory Effect of Dihydroartemisinin, An Active Ingredient of Artemisia annua, on Lipid Accumulation in Differentiating 3T3-L1 Preadipocytes" Journal of Korean Medicine for Obesity Research 20, no.1 (2020) : 1-9.doi: 10.15429/jkomor.2020.20.1.1
Jang,Byeong-Churl. Inhibitory Effect of Dihydroartemisinin, An Active Ingredient of Artemisia annua, on Lipid Accumulation in Differentiating 3T3-L1 Preadipocytes. Journal of Korean Medicine for Obesity Research, 20(1), 1-9. doi: 10.15429/jkomor.2020.20.1.1
Jang,Byeong-Churl. Inhibitory Effect of Dihydroartemisinin, An Active Ingredient of Artemisia annua, on Lipid Accumulation in Differentiating 3T3-L1 Preadipocytes. Journal of Korean Medicine for Obesity Research. 2020; 20(1) 1-9. doi: 10.15429/jkomor.2020.20.1.1
Jang,Byeong-Churl. Inhibitory Effect of Dihydroartemisinin, An Active Ingredient of Artemisia annua, on Lipid Accumulation in Differentiating 3T3-L1 Preadipocytes. 2020; 20(1), 1-9. Available from: doi:10.15429/jkomor.2020.20.1.1
Jang,Byeong-Churl. "Inhibitory Effect of Dihydroartemisinin, An Active Ingredient of Artemisia annua, on Lipid Accumulation in Differentiating 3T3-L1 Preadipocytes" Journal of Korean Medicine for Obesity Research 20, no.1 (2020) : 1-9.doi: 10.15429/jkomor.2020.20.1.1
