Protective Effect of Protocatechuic Acid, Phenolic Compound of Momordica Charantia, against Oxidative Stress and Neuroinflammation in C6 Glial Cell (여주의 페놀성 화합물인 Protocatechuic Acid의 산화적 스트레스 개선 및 신경염증 보호 효과)

Kim Ji-hyun (김지현); Jung Ran Choi (최정란); Eun Ju  Cho (조은주); Hyun Young Kim (김현영)

doi:10.15429/jkomor.2020.20.1.10

Protective Effect of Protocatechuic Acid, Phenolic Compound of Momordica Charantia, against Oxidative Stress and Neuroinflammation in C6 Glial Cell

Journal of Korean Medicine for Obesity Research
Abbr : J Korean Med Obes Res
2020, 20(1), pp.10-19
DOI : 10.15429/jkomor.2020.20.1.10
Publisher : The Society of Korean Medicine for Obesity Research
Research Area : Medicine and Pharmacy > Korean Medicine
Received : May 5, 2020
Accepted : June 10, 2020
Published : June 30, 2020

Kim Ji-hyun ¹, Jung Ran Choi ¹, Eun Ju Cho ¹, Hyun Young Kim ²

¹부산대학교 식품영양학과
²경남과학기술대학교 식품과학부

Accredited

ABSTRACT

Objectives: Oxidative stress-mediated neuroinflammation has been supposed as a crucial factor that contributes to the pathogenesis of many neurodegenerative diseases. In this study, we aimed to investigate the protective activity against oxidative stress and neuroinflammation of protocatechuic acid (PA), active phenolic compound from Momordica Charantia. Methods: Protective activity of PA from oxidative stress was performed under in vitro conditions. Our study investigated the protective mechanism of PA from neuroinflammation in cellular system using C6 glial cell. To investigate the improvement the effects on oxidative stress and neuroinflammation, we induced oxidative stress by H2O2 (100 μM) stimulation and induced neuroinflammation by treatment with lipopolysaccharide (LPS) (1 μg/mL) and interferon-gamma (IFN-γ) (10 ng/mL) in C6 glial cells. Results: PA showed strong radical scavenging effect against 1,1-dipenyl-2-picrylhydrazyl, hydroxy radical (⋅OH) and nitric oxide (NO). Under oxidative stress treated by H2O2, the result showed the increased mRNA expressions of oxidative stress markers such as nuclear factor-kappaB (NF-κB), cyclooxygenase (COX-2) and inducible nitric oxide (iNOS). However, the treatment of PA led to reduced mRNA expressions of NF-κB, COX-2 and iNOS. Moreover, PA attenuated the production of interleukin-6 and scavenged NO generated by both endotoxin LPS and IFN-γ together. Furthermore, it also reduced LPS and IFN-γ-induced mRNA expressions of iNOS and COX-2. Conclusions: In conclusion, our results collectively suggest that PA, phenolic compound of Momordica Charantia, could be a safe anti-oxidant and a promising anti-neuroinflammatory molecule for neurodegenerative diseases.

KEYWORDS

Neuroglia, Momordica charantia, Inflammation, Oxidative stress, Protocatechuic acid

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* References for papers published after 2023 are currently being built.

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This paper was written with support from the National Research Foundation of Korea.

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Journal of Korean Medicine for Obesity Research 2023 KCI Impact Factor : 0.43

Protective Effect of Protocatechuic Acid, Phenolic Compound of Momordica Charantia, against Oxidative Stress and Neuroinflammation in C6 Glial Cell

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* References for papers published after 2023 are currently being built.

Journal of Korean Medicine for Obesity Research 2023 KCI Impact Factor : 0.43

Protective Effect of Protocatechuic Acid, Phenolic Compound of Momordica Charantia, against Oxidative Stress and Neuroinflammation in C6 Glial Cell

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