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@article{ART002667674},
author={Hyo-Shin Kwon and Jang,Byeong-Churl},
title={Tanshinone I, an Active Ingredient of Salvia miltiorrhiza, Inhibits Differentiation of 3T3-L1 Preadipocytes and Lipid Accumulation in Zebrafish},
journal={Journal of Korean Medicine for Obesity Research},
issn={1976-9334},
year={2020},
volume={20},
number={2},
pages={109-121},
doi={10.15429/jkomor.2020.20.2.109}

TY - JOUR
AU - Hyo-Shin Kwon
AU - Jang,Byeong-Churl
TI - Tanshinone I, an Active Ingredient of Salvia miltiorrhiza, Inhibits Differentiation of 3T3-L1 Preadipocytes and Lipid Accumulation in Zebrafish
JO - Journal of Korean Medicine for Obesity Research
PY - 2020
VL - 20
IS - 2
PB - The Society of Korean Medicine for Obesity Research
SP - 109
EP - 121
SN - 1976-9334
AB - Objectives: Tanshinone I is a bioactive constituent in Salvia miltiorrhiza. At present, the anti-obesity effect and mechanism of tanshinone I are not fully understood. Here we investigated the effect of tanshinone I on lipid accumulation in 3T3-L1 preadipocytes and zebrafish. Methods: Lipid accumulation and triglyceride (TG) content in 3T3-L1 cells were determined by Oil Red O staining and AdipoRed assay, respectively. The expression and phosphorylation levels of adipogenic/lipogenic proteins in 3T3-L1 cells were evaluated by Western blotting. The messenger RNA (mRNA) expression levels of adipogenic/lipogenic markers and leptin in 3T3-L1 cells were measured by reverse transcription polymerase chain reaction (RT-PCR). Lipid accumulation in zebrafish was assessed by LipidGreen2 staining. Results: Tanshinone I at 5 μM largely blocked lipid accumulation and reduced TG content in differentiating 3T3-L1 cells. Furthermore, tanshinone I decreased the expression of CCAAT/enhancer-binding protein-α (C/EBP-α), peroxisome proliferator-activated receptor-γ (PPAR-γ), fatty acid synthase (FAS), acetyl CoA carboxylase (ACC), and perilipin A but also the phosphorylation of signal transducer and activator of transcription-3 (STAT-3) in differentiating 3T3-L1 cells. In addition, tanshinone I increased the phosphorylation of adenosine 3’,5’-cyclic monophosphate (cAMP)-activated protein kinase (AMPK) while decreased the intracellular adenosine triphosphate (ATP) content with no change in the phosphorylation and expression of liver kinase-B1 in differentiating 3T3-L1 cells. Importantly, tanshinone I also reduced the extent of lipid deposit formation in developing zebrafish. Conclusions: These findings demonstrate that tanshinone I has strong anti-adipogenic effects on 3T3-L1 cells and reduces adiposity in zebrafish, and these anti-adipogenic effect in 3T3-L1 cells are mediated through control of C/EBP-α, PPAR-γ, STAT-3, FAS, ACC, perilipin A, and AMPK.
KW - Tanshinone;Adipogenesis;AMP-activated protein kinases;3T3-L1 cells;Zebrafish
DO - 10.15429/jkomor.2020.20.2.109
ER -

Hyo-Shin Kwon and Jang,Byeong-Churl. (2020). Tanshinone I, an Active Ingredient of Salvia miltiorrhiza, Inhibits Differentiation of 3T3-L1 Preadipocytes and Lipid Accumulation in Zebrafish. Journal of Korean Medicine for Obesity Research, 20(2), 109-121.

Hyo-Shin Kwon and Jang,Byeong-Churl. 2020, "Tanshinone I, an Active Ingredient of Salvia miltiorrhiza, Inhibits Differentiation of 3T3-L1 Preadipocytes and Lipid Accumulation in Zebrafish", Journal of Korean Medicine for Obesity Research, vol.20, no.2 pp.109-121. Available from: doi:10.15429/jkomor.2020.20.2.109

Hyo-Shin Kwon, Jang,Byeong-Churl "Tanshinone I, an Active Ingredient of Salvia miltiorrhiza, Inhibits Differentiation of 3T3-L1 Preadipocytes and Lipid Accumulation in Zebrafish" Journal of Korean Medicine for Obesity Research 20.2 pp.109-121 (2020) : 109.

Hyo-Shin Kwon, Jang,Byeong-Churl. Tanshinone I, an Active Ingredient of Salvia miltiorrhiza, Inhibits Differentiation of 3T3-L1 Preadipocytes and Lipid Accumulation in Zebrafish. 2020; 20(2), 109-121. Available from: doi:10.15429/jkomor.2020.20.2.109

Hyo-Shin Kwon and Jang,Byeong-Churl. "Tanshinone I, an Active Ingredient of Salvia miltiorrhiza, Inhibits Differentiation of 3T3-L1 Preadipocytes and Lipid Accumulation in Zebrafish" Journal of Korean Medicine for Obesity Research 20, no.2 (2020) : 109-121.doi: 10.15429/jkomor.2020.20.2.109

Hyo-Shin Kwon; Jang,Byeong-Churl. Tanshinone I, an Active Ingredient of Salvia miltiorrhiza, Inhibits Differentiation of 3T3-L1 Preadipocytes and Lipid Accumulation in Zebrafish. Journal of Korean Medicine for Obesity Research, 20(2), 109-121. doi: 10.15429/jkomor.2020.20.2.109

Hyo-Shin Kwon; Jang,Byeong-Churl. Tanshinone I, an Active Ingredient of Salvia miltiorrhiza, Inhibits Differentiation of 3T3-L1 Preadipocytes and Lipid Accumulation in Zebrafish. Journal of Korean Medicine for Obesity Research. 2020; 20(2) 109-121. doi: 10.15429/jkomor.2020.20.2.109

Hyo-Shin Kwon, Jang,Byeong-Churl. Tanshinone I, an Active Ingredient of Salvia miltiorrhiza, Inhibits Differentiation of 3T3-L1 Preadipocytes and Lipid Accumulation in Zebrafish. 2020; 20(2), 109-121. Available from: doi:10.15429/jkomor.2020.20.2.109

Hyo-Shin Kwon and Jang,Byeong-Churl. "Tanshinone I, an Active Ingredient of Salvia miltiorrhiza, Inhibits Differentiation of 3T3-L1 Preadipocytes and Lipid Accumulation in Zebrafish" Journal of Korean Medicine for Obesity Research 20, no.2 (2020) : 109-121.doi: 10.15429/jkomor.2020.20.2.109